A5010547713- Metalloenzymes and iron-sulfur proteins
- Enzyme Structure and Function
- Metal-Catalyzed Oxygenation Mechanisms
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Electrocatalysts for Energy Conversion
- Enzyme Catalysis and Immobilization
- Microbial Fuel Cells and Bioremediation
- Hydrogen Storage and Materials
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Hemoglobin structure and function
- Amino Acid Enzymes and Metabolism
- Anaerobic Digestion and Biogas Production
- Electron Spin Resonance Studies
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Porphyrin Metabolism and Disorders
- Biochemical and Molecular Research
- Chromium effects and bioremediation
- Radioactive element chemistry and processing
University of Georgia
2016-2025
University of Sheffield
2023
University of North Carolina at Chapel Hill
2022
University of Atlanta
2021
University of Tennessee at Knoxville
2020
Cell Biotech (South Korea)
2020
Oak Ridge National Laboratory
1999-2018
Synthetic Biologics (United States)
2018
Kookmin University
2018
University of California, Davis
1996-2017
The crystal structure of the tungsten-containing aldehyde ferredoxin oxidoreductase (AOR) from Pyrococcus furiosus , a hyperthermophilic archaeon (formerly archaebacterium) that grows optimally at 100°C, has been determined 2.3 angstrom resolution by means multiple isomorphous replacement and form averaging. AOR consists two identical subunits, each containing an Fe 4 S cluster molybdopterin-based tungsten cofactor is analogous to molybdenum found in large class oxotransferases. Whereas...
The hyperthermophilic and anaerobic bacterium Thermotoga maritima ferments a wide variety of carbohydrates, producing acetate, CO(2), H(2). Glucose is degraded through classical Embden-Meyerhof pathway, both NADH reduced ferredoxin are generated. oxidation these electron carriers must be coupled to H(2) production, but the mechanism by which this occurs unknown. trimeric [FeFe]-type hydrogenase that was previously purified from T. does not use either or as sole donor. This problem has now...
Most fungi and bacteria degrade plant cell walls by secreting free, complementary enzymes that hydrolyze cellulose; however, some use large enzymatic assemblies called cellulosomes, which recruit to protein scaffolds. The thermophilic bacterium Caldicellulosiruptor bescii uses an intermediate strategy, many free cellulases contain multiple catalytic domains. One of these, CelA, comprises a glycoside hydrolase family 9 48 domain, as well three type III cellulose-binding modules. In the...
Significance Hydrogen (H 2 ) has great potential to be used power passenger vehicles. One solution these problems is distribute and store renewable carbohydrate instead, converting it hydrogen as required. In this work more than 10 purified enzymes were combined into artificial enzymatic pathways a high yield from both glucose xylose was achieved. Also, gaseous can separated aqueous substrates easily, greatly decreasing product separation costs, avoid reconcentrating sugar solutions. This...
Superoxide reductase from the hyperthermophilic anaerobe Pyrococcus furiosus uses electrons reduced nicotinamide adenine dinucleotide phosphate, by way of rubredoxin and an oxidoreductase, to reduce superoxide hydrogen peroxide, which is then water peroxidases. Unlike dismutase, enzyme that protects aerobes toxic effects oxygen, SOR does not catalyze production oxygen therefore confers a selective advantage on anaerobes. associated proteins are catalytically active 80°C below optimum growth...
The archaebacterium, Pyrococcus furiosus, grows optimally at 100 degrees C by a fermentative type metabolism in which H2 and CO2 are the only detectable products. organism also reduces elemental sulfur (S0) to H2S. Cells grown absence of S0 contain single hydrogenase, located cytoplasm, has been purified 350-fold apparent homogeneity. yield evolution activity from reduced methyl viologen 80 was 40%. hydrogenase Mr value 185,000 +/- 15,000 is composed three subunits 46,000 (alpha), 27,000...
Oxidative phosphorylation involves the coupling of ATP synthesis to proton-motive force that is generated typically by a series membrane-bound electron transfer complexes, which ultimately reduce an exogenous terminal acceptor. This not case with Pyrococcus furiosus , archaeon grows optimally near 100°C. It has anaerobic respiratory system consists single enzyme, hydrogenase. Moreover, it does require added acceptor as enzyme reduces protons, simplest acceptors, hydrogen gas using electrons...
Tungsten (atomic number 74) and the chemically analogous very similar metal molybdenum 42) are minor yet equally abundant elements on this planet. The essential role of in biology has been known for decades molybdoenzymes ubiquitous. Yet, it is only recently that a biological tungsten established prokaryotes, although not as eukaryotes. best characterized organisms with regard to their metabolism certain species hyperthermophilic archaea (Pyrococcus furiosus Thermococcus litoralis),...
The anaerobic archaebacterium, Pyrococcus furiosus, grows optimally at 100 degrees C by a fermentative-type metabolism in which H2, CO2, and organic acids are end products. growth of this organism is stimulated tungsten, and, from it, novel, red-colored, tungsten-iron-sulfur protein, abbreviated RTP, has been purified (Mukund, S., Adams, M. W. (1990) J. Biol. Chem. 265, 11508-11516). RTP (Mr approximately 85,000) contained 1W, 7Fe, 5 acid-labile sulfide atoms/molecule exhibited unique EPR...
BackgroundThe future hydrogen economy offers a compelling energy vision, but there are four main obstacles: production, storage, and distribution, as well fuel cells. Hydrogen production from inexpensive abundant renewable biomass can produce cheaper hydrogen, decrease reliance on fossil fuels, achieve zero net greenhouse gas emissions, current chemical biological means suffer low yields and/or severe reaction conditions.Methodology/Principal FindingsHere we demonstrate synthetic enzymatic...
Pyrococcus furiosus ferredoxin is the only known example of a containing single [4Fe-4S] cluster that has non-cysteinyl ligation one iron atom, as evidenced by replacement ligating cysteine residue an aspartic acid in amino sequence. The properties iron-sulfur both aerobically and anaerobically isolated have been characterized EPR, magnetic circular dichroism, resonance Raman spectroscopies. ferrodoxin contains [4Fe-4S]+,2+ with anomalous oxidized reduced states which are attributed to...