A5038821821- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Polydiacetylene-based materials and applications
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Advanced X-ray Imaging Techniques
- Supramolecular Self-Assembly in Materials
- Glycosylation and Glycoproteins Research
- X-ray Spectroscopy and Fluorescence Analysis
- Allergic Rhinitis and Sensitization
- Chemical Synthesis and Analysis
- Advanced Electron Microscopy Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Food Allergy and Anaphylaxis Research
Diamond Light Source
2015-2024
Didcot Community Hospital
2017-2019
Lawrence Berkeley National Laboratory
2007-2014
Oklahoma Medical Research Foundation
2013
Howard Hughes Medical Institute
1999-2004
Institute of Molecular Biology and Biophysics
2000
Yale University
1998-1999
Iowa State University
1964
University of Arizona
1964
Abstract Unstructured proteins, RNA or DNA components provide functionally important flexibility that is key to many macromolecular assemblies throughout cell biology. As objective, quantitative experimental measures of and disorder in solution are limited, small angle scattering (SAS), particular X‐ray (SAXS), provides a critical technology assess as well shape assembly. Here, we consider the Porod‐Debye law powerful tool for detecting biopolymer SAS experiments. We show region...
The phytohormone abscisic acid (ABA) acts in seed dormancy, plant development, drought tolerance, and adaptive responses to environmental stresses. Structural mechanisms mediating ABA receptor recognition signaling remain unknown but are essential for understanding manipulating abiotic stress resistance. Here, we report structures of pyrabactin resistance 1 (PYR1), a prototypical PYR/PYR1-like (PYL)/regulatory component (RCAR) protein that functions early signaling. crystallographic...
Specific tertiary structural motifs determine the complete architecture of RNA molecules (see picture for examples). Within last few years a number high-resolution crystal structures complex RNAs have led to new insights into mechanisms by which these folds are attained. In this review and how they influence folding pathway biological discussed, as well developments in modeling structure based upon findings.
SMARCAL1 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A-like1) maintains genome integrity during DNA replication. Here we investigated its mechanism action. We found that travels with elongating replication forks, and absence leads to MUS81-dependent double-strand break formation. Binding specific nucleic acid substrates activates activity in a reaction requires HARP2 (Hep-A-related protein 2) domain. Homology modeling indicates the HARP domain is...
The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by US Department Energy and Institutes Health, is optimized for both small-angle X-ray scattering (SAXS) macromolecular crystallography (MX), making it unique among world's mostly SAXS or MX dedicated beamlines. Since was commissioned, assessments limitations advantages a combined have suggested new strategies integration optimal data collection methods led to additional hardware...
DNA double strand break (DSB) repair by non-homologous end joining (NHEJ) is initiated DSB detection Ku70/80 (Ku) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) recruitment, which promotes pathway progression through poorly defined mechanisms. Here, Ku DNA-PKcs solution structures alone in complex with DNA, x-ray scattering, reveal major structural reorganizations that choreograph NHEJ initiation. The Ku80 C-terminal region forms a flexible arm extends from the DNA-binding...
B21 is a small-angle X-ray scattering (SAXS) beamline with bending magnet source in the 3 GeV storage ring at Diamond Light Source Ltd synchrotron UK. The utilizes double multi-layer monochromator and toroidal focusing optic to deliver 2 × 10 12 photons per second 34 40 µm (FWHM) focal spot in-vacuum Eiger 4M (Dectris) detector. A high-performance liquid chromatography system liquid-handling robot make it possible load solution samples into temperature-controlled sample cell high level of...
Abstract The CRISPR system provides adaptive immunity against mobile genetic elements in prokaryotes. On binding invading RNA species, Type III systems generate cyclic oligoadenylate (cOA) signalling molecules, potentiating a powerful immune response by activating downstream effector proteins, leading to viral clearance, cell dormancy or death. Here we describe the structure and mechanism of cOA-activated defence DNA endonuclease, ancillary nuclease 1 (Can1). Can1 has unique monomeric with...
By coupling the protection and organization of single-stranded DNA (ssDNA) with recruitment alignment processing factors, replication protein A (RPA) lies at heart dynamic multi-protein machinery. Nevertheless, how RPA coordinates biochemical functions its eight domains remains unknown. We examined structural biochemistry RPA's DNA-binding activity, combining small-angle X-ray neutron scattering all-atom molecular dynamics simulations to investigate architecture core. The data reveal...
Defining the shape, conformation, or assembly state of an RNA in solution often requires multiple investigative tools ranging from nucleotide analog interference mapping to X-ray crystallography. A key addition this toolbox is small-angle scattering (SAXS). SAXS provides direct structural information regarding size, and flexibility particle has proven powerful for analyses structures with minimal requirements sample concentration volumes. In principle, can provide reliable data on small...