A5021699923- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Estrogen and related hormone effects
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Dutch Social and Cultural Studies
- Physiological and biochemical adaptations
- Protein Kinase Regulation and GTPase Signaling
- Aquaculture disease management and microbiota
- Metabolism, Diabetes, and Cancer
- Bone Metabolism and Diseases
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- Stress Responses and Cortisol
- Ubiquitin and proteasome pathways
- Biomimetic flight and propulsion mechanisms
- Medical Imaging Techniques and Applications
- Innovative Teaching Methods
- Signaling Pathways in Disease
University of California, Davis
2015-2023
San Diego State University
2012-2017
Salk Institute for Biological Studies
2009-2015
University of Copenhagen
2014
Wageningen University & Research
2004-2008
Institute of Zoology of the Slovak Academy of Sciences
2005
Complex local crosstalk amongst endocrine cells within the islet ensures tight coordination of their output. This is illustrated by recent demonstration that negative feedback control delta pancreatic islets determines homeostatic set-point for plasma glucose during mouse postnatal development. However, close association facilitates paracrine also complicates distinction between effects mediated directly on beta from indirect via intermediates, such as somatostatin cells.To resolve this...
Insulin producing beta cell and glucagon alpha cells are colocalized in pancreatic islets an arrangement that facilitates the coordinated release of two principal hormones regulate glucose homeostasis prevent both hypoglycemia diabetes. However, this intricate organization has also complicated determination cellular source(s) expression genes detected islet. This reflects a significant gap our understanding mouse islet physiology, which reduces effectiveness by mice model human disease.To...
The peptide hormone Urocortin 3 (Ucn 3) is abundantly and exclusively expressed in mouse pancreatic beta cells where it regulates insulin secretion. Here we demonstrate that Ucn first appears at embryonic day (E) 17.5 and, from approximately postnatal (p) 7 onwards throughout adult life, becomes a unifying exclusive feature of cells. These observations identify as potential cell maturation marker. To determine whether similarly restricted to humans, conducted comprehensive...
Abstract One of the ultimate goals regenerative medicine is generation patient-specific organs from pluripotent stem cells (PSCs). Sheep are potential hosts for growing human through technique blastocyst complementation. We report here creation pancreatogenesis-disabled sheep by oocyte microinjection CRISPR/Cas9 targeting PDX1 , a critical gene pancreas development. compared efficiency target mutations after microinjecting system in metaphase II (MII) oocytes and zygote stage embryos. MII...
Corticotropin-releasing factor (CRF), originally characterized as the principal neuroregulator of hypothalamus-pituitary-adrenal axis, has broad central and peripheral distribution actions. We demonstrate presence CRF receptor type 1 (CRFR1) on primary beta cells show that activation pancreatic CRFR1 promotes insulin secretion, thus contributing to restoration normoglycemic equilibrium. Stimulation initiates a cAMP response secretion in vitro vivo leads phosphorylation element binding...
Three novel CXC chemokines were identified in common carp ( Cyprinus carpio L.) through homology cloning. Phylogenetic analyses show that one of the three is an unambiguous orthologue CXCL14 , whereas both others are orthologues CXCL12 and named CXCL12a CXCL12b . Percentages amino acid identity between each these their human mouse markedly higher than those reported previously for other chemokines, suggestive involvement vital processes, which have allowed relatively few structural changes....
Mechanical load is an important factor in the differentiation of cells and tissues. To investigate effects increased mechanical on development muscle bone, zebrafish were subjected to endurance swim training for 6 h/day 10 wk starting at 14 days after fertilization. During first 3 training, trained fish showed transiently growth compared with untrained (control) fish. Increased expression proliferating cell nuclear antigen suggests that this realized part through proliferation. Red white...
Abstract Background High throughput sequencing has enabled the interrogation of transcriptomic landscape glucagon-secreting alpha cells, insulin-secreting beta and somatostatin-secreting delta cells. These approaches have furthered our understanding expression patterns that define healthy or diseased islet cell types helped explicate some intricacies between major crosstalk glucose regulation. All three endocrine derive from a common pancreatic progenitor, yet cells partially opposing...
Proliferation of pancreatic β-cells has long been known to reach its peak in the neonatal stages and decline during adulthood. However, β-cell proliferation studied under assumption that all constitute a single, homogenous population. It is unknown whether subpopulation retains capacity proliferate at higher rate thus contributes disproportionately maintenance mature mass adults. We therefore assessed proliferative turnover potential virgin β-cells, novel population immature found islet...
Abstract Urocortin 3 (Ucn 3), member of the corticotropin-releasing factor (CRF) family peptide hormones, is released from β-cells to potentiate insulin secretion. Ucn activates CRF type-2 receptor (CRFR2) but does not activate type-1 (CRFR1), which was recently demonstrated on β-cells. While direct actions secretion suggest presence cognate receptors within islet microenvironment, this has been established. Here we demonstrate that CRFR2α expressed by MIN6 insulinoma cells and primary mouse...
The fast muscle fibres in the anterior trunk of teleost fish are primarily responsible for large amplitude undulatory swimming motions. Previous theoretical studies suggested that near-helical arrangement these results a (fairly) uniform distribution fibre strain and work output during swimming. However, underlying simplifications precluded unequivocal support this hypothesis. We studied muscle-fibre reorientation concomitant myotomal variance body segment near anus larval juvenile...
During the development of diabetes β-cells are exposed to elevated concentrations proinflammatory cytokines, TNFα and IL1β, which in vitro induce β-cell death. The class B G-protein-coupled receptors (GPCRs): corticotropin-releasing factor receptor 1 (CRFR1) CRFR2 expressed pancreatic islets. As downstream signaling by other GPCRs can protect against cytokine-induced apoptosis, we evaluated protective potential CRFR activation a pro-inflammatory setting. CRFR1/CRFR2 ligands activated AKT...
There is great interest in generating functionally mature beta cells from stem cells, as loss of functional cell mass contributes to the pathophysiology diabetes. Identifying markers maturity therefore very helpful for distinguishing that have been successfully differentiated into fully did not. Urocortin 3 (UCN3) a peptide hormone whose expression associated with acquisition cells. The onset its occurs after other are already expressed and marks beginning dedifferentiation. Its pattern...
The present paper discusses the effects of decreased muscle activity (DMA) on embryonic development in zebrafish. Wild-type zebrafish embryos become mobile around 18 h post-fertilisation, long before axial musculature is fully differentiated. As a model for DMA, nic(b107) mutant was used. In embryos, fibres are mechanically intact and able to contract, but neuronal signalling defective not activated, rendering immobile. Despite immobility, distinguished slow fast developed at correct...
Grb2 and ShcA are two phosphotyrosine‐binding proteins that link receptor protein‐tyrosine kinases to activation of the Ras‐Erk pathway. While some receptors bind directly, others ShcA, which provides a binding site for Grb2. In order compare signal transduction through Grb2‐binding with ShcA‐binding site, we replaced in NGF site. Our results show Grb2‐ sites have similar abilities activate PI 3‐kinase‐Akt pathways. contrast, they displayed dramatic differences their ability DNA synthesis.