A5074639486- Cardiovascular Function and Risk Factors
- Heart Failure Treatment and Management
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Renin-Angiotensin System Studies
- Circular RNAs in diseases
- Hormonal Regulation and Hypertension
- Cardiac Imaging and Diagnostics
- Acute Myocardial Infarction Research
- Cardiac electrophysiology and arrhythmias
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Genetic Associations and Epidemiology
- Apelin-related biomedical research
- Cardiac Fibrosis and Remodeling
- Acute Kidney Injury Research
- Cardiovascular Health and Risk Factors
- GDF15 and Related Biomarkers
- RNA Research and Splicing
- Cardiac Arrest and Resuscitation
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Blood Pressure and Hypertension Studies
- Coronary Interventions and Diagnostics
University of Otago
2015-2024
New Zealand Brain Research Institute
2013-2024
Christ University
2024
American Nephrology Nurses Association
2012-2017
Cleveland Clinic
2016
Salk Institute for Biological Studies
2010-2016
American Heart Association
2014-2015
Brigham and Women's Hospital
2015
Broad Institute
2015
Harvard University
2015
Abstract Aims Inflammation plays an important role in cardiovascular disease (CVD) development. The NOD-like receptor protein-3 (NLRP3) inflammasome contributes to the development of atherosclerosis animal models. Components NLRP3 pathway such as interleukin-1β can therapeutically be targeted. Associations genetically determined inflammasome-mediated systemic inflammation with CVD and mortality humans are unknown. Methods results We explored association genetic variants prevalent 538 167...
Heart failure (HF) is the most common long-term complication of acute myocardial infarction (MI). Understanding plasma proteins associated with post-MI HF and their gene expression may identify new candidates for biomarker drug target discovery.We used aptamer-based affinity-capture proteomics to measure 1305 at 1 month in a New Zealand cohort (CDCS [Coronary Disease Cohort Study]) including 181 patients who were subsequently hospitalized comparison 250 remained event free over median...
AimsPlasma aldosterone levels have been shown to be associated with adverse clinical outcomes after ST-elevation myocardial infarction (STEMI). We investigated whether in patients presenting STEMI or non-STEMI, are predictive of mortality during prolonged follow-up.
After myocardial infarction (MI), the heart may undergo progressive ventricular remodeling, resulting in a deterioration of cardiac function. TGF-β is key cytokine that both initiates and terminates tissue repair, its sustained production underlies development fibrosis, particularly after MI. We investigated effects novel orally active specific inhibitor receptor 1 (SD-208) an experimental model Mice underwent ligation left coronary artery to induce MI were subsequently treated for 30 d with...
Background and Aims The methylene-tetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L) gene is involved in mitochondrial tetrahydrofolate metabolism. Polymorphisms MTHFD1L, including rs6922269, have been implicated risk for coronary artery disease (CAD). We investigated the association between rs6922269 known metabolic factors survival two independent cohorts of heart patients. Methods Results DNA plasma from 1940 patients with acute syndromes were collected a median 32 days...
Chromosome position 9p21 encodes three-tumor suppressors p16INK4a, p14ARF, and p15INK4b, the long non-coding RNA ANRIL (antisense noncoding in INK4 locus). The rs11515 single nucleotide polymorphism p16INK4a/p14ARF 3′ untranslated region is associated with glioblastoma, melanoma, other cancers. This study investigated frequency effect of genotypes breast cancer. Genomic DNA samples from 400 women (200 200 without a diagnosis cancer) were genotyped for major © minor (G) alleles. was also 108...
Angiotensinogen M235T and T174M polymorphisms have individually been associated with elevated levels of plasma angiotensinogen, hypertension, left ventricular hypertrophy. In this study, heart failure patients (n=451) were genotyped for the angiotensinogen to investigate association survival (recorded over 4 years follow-up) prognostic hormone markers. Patients carrying 235TT genotype (n=86) 3 younger at admission (P=0.011), and, in those diagnosis was made approximately 10 earlier than...
Reliability of real-time PCR (RT-qPCR) data is dependent on the use appropriate reference gene(s) for normalization. To date, no validated genes have been reported normalizing gene expression in human myocardium. This study aimed to identify studies failed and non-failed myocardium.Bioinformatic analysis published heart arrays (195 hearts, 16 donor hearts) was used 10 stable abundant further testing. The stability these investigated 28 myocardium samples by RT-qPCR using geNorm...
Background— Chromosome 9p21.3 (chr9p21.3) recently was identified by several genome-wide association studies as the genomic region most strongly associated with risk of coronary artery disease. Within chr9p21.3 locus, single-nucleotide polymorphism rs1333049 has been demonstrated to be susceptibility developing However, effect on clinical outcomes in patients established disease yet determined. Methods and Results— Coronary Disease Cohort Study (CDCS) (n=1054) Post-Myocardial Infarction...
Genome-wide association studies have identified a coronary artery disease (CAD) risk locus in non-coding region at 9p21.3, the nearest genes being CDKN2A and CDKN2B. To understand pathways by which this might influence CAD susceptibility, we investigated associations between 9p21.3 genotype global gene expression heart tissue from donors with no diagnosed (n = 108, predominant cause of death, cerebral vascular accident) carotid plaque 106), aorta 104) mammary 88) tissues valve endarterectomy...
Genome-wide association studies have identified gene variants associated with coronary artery disease risk; however, whether they affect progression is largely unknown. This study investigated associations between polymorphisms at 1p13.3 (rs599839), 1q41 (rs17465637), and 3q22.3 (rs9818870) cardiovascular outcomes in healthy volunteers patients established heart disease.Canterbury Healthy Volunteer (HV) (n=1649), Coronary Disease Cohort Study (CDCS) (n=1797), Post-Myocardial Infarction (PMI)...
Oxidative stress plays a critical role in the pathogenesis of cardiovascular disease and diabetes. Studies vascular cells experimental animals have demonstrated that angiotensin type-1 receptor (AT1R) contributes to formation reactive oxygen species by activating nicotinamide-adenine dinucleotide phosphate oxidases, but relevance this pathway human heart has not been established. Here we demonstrate polymorphism AT1R gene (A1166C), linked increased activity, is associated with elevated...
Hydrogen sulfide (H2S) is recognized as an endogenous gaseous signaling molecule generated by cystathionine γ-lyase (CSE) in cardiovascular tissues. H2S up-regulation has been shown to reduce ischemic injury, and donors are cardioprotective rodent models when administered concurrent with myocardial ischemia. We evaluated the potential utility of therapy ameliorating cardiac remodeling administration delayed until 2 h post-infarction mice or without gene deletion (CSE−/−). The slow-release...
Plasma cardiac markers may assist in prediction of incident cardiovascular disease.The incremental value Troponins (T and I) NT-proBNP added to risk factors the PREDICT score for disease (CVD) primary care, was assessed 4102 asymptomatic participants a randomised controlled trial Vitamin D (ViDA). Findings were corroborated 2528 separate community-based observational registry CVD-free volunteers (HVOLS).Hazard ratios first events adjusted factors, comparing fifth quintiles marker plasma...
Abstract Background Individuals born very low birthweight (VLBW) are at increased risk of impaired cardiovascular and respiratory function in adulthood. To identify markers to predict future for VLBW individuals, we analyzed DNA methylation birth 28 years the New Zealand (NZ) cohort (all infants < 1500 g NZ 1986) compared with age-matched, normal controls. Associations between neonatal cardiac structure (echocardiography), vascular outcomes age were documented. Results Genomic from...
Mass spectrometry is a powerful technique for investigating renal pathologies and identifying biomarkers, efficient protein extraction from kidney tissue essential bottom-up proteomic analyses. Detergent-based strategies aid cell lysis solubilization but are poorly compatible with downstream digestion liquid chromatography-coupled mass spectrometry, requiring additional purification buffer-exchange steps. This study compares two well-established detergent-based methods (in-solution sodium...