Ralph Burkhardt
A5022779311- Genetic Associations and Epidemiology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Metabolomics and Mass Spectrometry Studies
- Lipoproteins and Cardiovascular Health
- Atrial Fibrillation Management and Outcomes
- Cholesterol and Lipid Metabolism
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and disorders
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Disease and Adiposity
- Cardiovascular Function and Risk Factors
- Liver Disease Diagnosis and Treatment
- Renal Diseases and Glomerulopathies
- Long-Term Effects of COVID-19
- Hematopoietic Stem Cell Transplantation
- Sphingolipid Metabolism and Signaling
- Genetic Mapping and Diversity in Plants and Animals
- Chronic Kidney Disease and Diabetes
- Lipid metabolism and biosynthesis
- Hemoglobinopathies and Related Disorders
- Diabetes, Cardiovascular Risks, and Lipoproteins
University Hospital Regensburg
2018-2025
University Hospital Leipzig
2011-2024
Friedrich Schiller University Jena
2024
Jena University Hospital
2024
German Medical Association
2024
University of Regensburg
2024
Leipzig University
2014-2023
Klinikum rechts der Isar
2023
Technical University of Munich
2023
Life University
2015-2017
The LIFE-Adult-Study is a population-based cohort study, which has recently completed the baseline examination of 10,000 randomly selected participants from Leipzig, major city with 550,000 inhabitants in east Germany. It first study this kind and size an urban population eastern part conducted by Leipzig Research Centre for Civilization Diseases (LIFE). Our objective to investigate prevalences, early onset markers, genetic predispositions, role lifestyle factors civilization diseases,...
Increased levels of the urinary albumin-to-creatinine ratio (UACR) are associated with higher risk kidney disease progression and cardiovascular events, but underlying mechanisms incompletely understood. Here, we conduct trans-ethnic (n = 564,257) European-ancestry specific meta-analyses genome-wide association studies UACR, including ancestry- diabetes-specific analyses, identify 68 UACR-associated loci. Genetic correlation analyses score associations in an independent electronic medical...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic challenges national health systems and the global economy. Monitoring of infection rates seroprevalence can guide public measures to combat pandemic. This depends on reliable tests active former infections. Here, we set out develop validate a specific sensitive enzyme linked immunosorbent assay (ELISA) for detection anti-SARS-CoV-2 antibody levels.In our ELISA, used SARS-CoV-2 receptor-binding domain (RBD) stabilized...
Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve survival time patients, who are often diagnosed too late at an incurable stage. Several studies report dysregulation lipid metabolism in tumor cells, suggesting that changes blood lipidome accompany growth. Here we show comprehensive mass spectrometric determination a wide range serum lipids reveals statistically significant differences between pancreatic patients and healthy controls, as...
Recombinant granulocyte/macrophage-colony-stimulating factor (rGM-CSF), prepared from Chinese hamster ovary (CHO) cells and Escherichia coli, was administered to 35 patients with the borderline polar lepromatous forms of leprosy by intradermal subcutaneous routes at doses 7.5-45.0 micrograms/d for 10 d. With each these routes, increases in number circulating eosinophils were noted. After injection, local skin sites demonstrated zones roughening micronodularity that appeared within 24-48 h...
Recent genome-wide association studies have identified a genetic locus at human chromosome 8q24 as having minor alleles associated with lower levels of plasma triglyceride (TG) and LDL cholesterol (LDL-C), higher HDL-C, well decreased risk for myocardial infarction. This contains only one annotated gene, tribbles homolog 1 (TRIB1), which has not previously been implicated in lipoprotein metabolism. Here we demonstrate role Trib1 regulator metabolism mice. Hepatic-specific overexpression...
Objective Alcohol-related pancreatitis is associated with a disproportionately large number of hospitalisations among GI disorders. Despite its clinical importance, genetic susceptibility to alcoholic chronic (CP) poorly characterised. To identify risk genes for CP and evaluate their relevance in non-alcoholic CP, we performed genome-wide association study functional characterisation new locus. Design 1959 European patients population-based controls from the KORA, LIFE INCIPE studies...
Genetics of gene expression (eQTLs or QTLs) has proved an indispensable tool for understanding biological pathways and pathomechanisms trait-associated SNPs. However, power most genome-wide eQTL studies is still limited. We performed a large study in peripheral blood mononuclear cells 2112 individuals increasing the to detect trans-effects genome-wide. Going beyond univariate SNP-transcript associations, we analyse relations eQTLs pathways, polygenetic effects regulation, trans-clusters...
Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) gut microbiota relevant for microbe⁻host communication. Here, we present a method quantification SCFAs in fecal samples liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The includes acetate, propionate, butyrate, isobutyrate with run time 4 min. reproducible (coefficients variation (CV) below 10%)...
Abstract Aims Inflammation plays an important role in cardiovascular disease (CVD) development. The NOD-like receptor protein-3 (NLRP3) inflammasome contributes to the development of atherosclerosis animal models. Components NLRP3 pathway such as interleukin-1β can therapeutically be targeted. Associations genetically determined inflammasome-mediated systemic inflammation with CVD and mortality humans are unknown. Methods results We explored association genetic variants prevalent 538 167...
Lipidomics data require consideration of ions with near-identical masses, which comprises among others the Type-II isotopic overlap. This overlap occurs in series lipid species differing only by number double bonds (DBs) mainly because natural abundance 13C-atoms. High-resolution mass spectrometry, such as Fourier-transform spectrometry (FTMS), is capable resolving depending on power. In this work, we evaluated FTMS quantification accuracy affected Spike experiments pairs various classes...
Journal Article Cohort Profile: The LIFE-Adult-Study Get access Christoph Engel, Engel Institute for Medical Informatics, Statistics and Epidemiology, Leipzig University, Leipzig, GermanyLeipzig Research Centre Civilization Diseases, Germany Corresponding author. Haertelstrasse 16–18, 04107 Germany. E-mail: christoph.engel@imise.uni-leipzig.de https://orcid.org/0000-0002-7247-282X Search other works by this author on: Oxford Academic PubMed Google Scholar Kerstin Wirkner, Wirkner Samira...
A key element of successful lipidomics analysis is a sufficient extraction lipid molecules typically by two-phase systems such as chloroform-based Bligh and Dyer (B&D). However, numerous metabolomics studies today apply easy to use one-phase extractions. In this work, quantitative flow injection high-resolution mass spectrometry was applied benchmark the recovery popular methods for human plasma samples. The following organic solvents were investigated: methanol (MeOH), ethanol (EtOH),...
Understanding normal aging of kidney function is pivotal to help distinguish individuals at particular risk for chronic disease. Glomerular filtration rate (GFR) typically estimated via serum creatinine (eGFRcrea) or cystatin C (eGFRcys). Since population-based age-group-specific reference values eGFR and eGFR-decline are scarce, we aimed provide such from data a wide age range. In four German cohorts (KORA-3, KORA-4, AugUR, DIACORE), participants underwent medical exams, interview, blood...
It has been argued that the limited genetic diversity and reduced allelic heterogeneity observed in isolated founder populations facilitates discovery of loci contributing to both Mendelian complex disease. A strong effect, severe isolation, substantial inbreeding have dramatically natives from island Kosrae, Federated States Micronesia, who exhibit a high prevalence obesity other metabolic disorders. We hypothesized drift possibly natural selection on Kosrae might increased frequency...
Summary The genetic basis of sleep is still poorly understood. Despite the moderate to high heritability sleep‐related phenotypes, known variants explain only a small proportion phenotypical variance. However, most previous studies were based solely upon self‐report measures. present study aimed conduct first genome‐wide association (GWA) actigraphic phenotypes. analyses included 956 middle‐ older‐aged subjects (40–79 years) from LIFE Adult Study. SenseWear Pro 3 Armband was used collect 11...
Abstract Treatment of advanced melanoma with combined PD-1/CTLA-4 blockade commonly causes serious immune-mediated complications. Here, we identify a subset patients predisposed to immune checkpoint blockade-related hepatitis who are distinguished by chronic expansion effector memory CD4 + T cells (T EM cells). Pre-therapy cell occurs primarily during autumn or winter in metastatic disease and high cytomegalovirus (CMV)-specific serum antibody titres. These clinical features implicate...