A5023184639- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Cardiovascular Disease and Adiposity
- Neural Networks and Applications
- Cardiac Imaging and Diagnostics
- Extracellular vesicles in disease
- Fuzzy Logic and Control Systems
- Signaling Pathways in Disease
- Cellular Mechanics and Interactions
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiovascular Function and Risk Factors
- Mass Spectrometry Techniques and Applications
- Trace Elements in Health
- Eicosanoids and Hypertension Pharmacology
- Viral Infectious Diseases and Gene Expression in Insects
- Heavy Metal Exposure and Toxicity
- Blood Coagulation and Thrombosis Mechanisms
- Cardiac Fibrosis and Remodeling
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Control Systems and Identification
- Sphingolipid Metabolism and Signaling
- Celiac Disease Research and Management
- Biosensors and Analytical Detection
- Advanced Sensor and Control Systems
National University of Singapore
2015-2025
National Heart Centre Singapore
2024
Duke-NUS Medical School
2024
National University Health System
2018-2023
National University Heart Centre Singapore
2015-2022
Monash Health
2021
National University Hospital
2016-2020
Macquarie University
2012
We identified a plasma signature of 11 C14 to C26 ceramides and 1 C16 dihydroceramide predictive major adverse cardiovascular events in patients with acute myocardial infarction (AMI). Among undergoing coronary artery bypass surgery, those recent AMI, compared without showed significant increase 5 the signature's 12 but not simultaneously-biopsied aortic tissue. In contrast, rat AMI model, sham control, concentrations all up-regulation 3 ceramide-producing enzymes, suggesting ischemic...
Mass spectrometry (MS)-based targeted lipidomics enables the robust quantification of selected lipids under various biological conditions but comprehensive software tools to support such analyses are lacking. Here we present LipidCreator, a that fully supports assay development. LipidCreator offers framework compute MS/MS fragment masses for over 60 lipid classes. provides all functionalities needed define fragments, manage stable isotope labeling, optimize collision energy and generate in...
Heart failure (HF) is the most common long-term complication of acute myocardial infarction (MI). Understanding plasma proteins associated with post-MI HF and their gene expression may identify new candidates for biomarker drug target discovery.We used aptamer-based affinity-capture proteomics to measure 1305 at 1 month in a New Zealand cohort (CDCS [Coronary Disease Cohort Study]) including 181 patients who were subsequently hospitalized comparison 250 remained event free over median...
Quantitative MS of human plasma lipids is a promising technology for translation into clinical applications. Current MS-based lipidomic methods rely on either direct infusion (DI) or chromatographic lipid separation (including reversed phase and hydrophilic interaction LC). However, the use markers in laboratory medicine limited by lack reference values, largely because considerable differences concentrations measured different laboratories worldwide. These inconsistencies can be explained...
Abstract Aims To identify differences in CT-derived perivascular (PVAT) and epicardial adipose tissue (EAT) characteristics that may indicate inflammatory status between post-treatment acute myocardial infarction (AMI) stable coronary artery disease (CAD) patients. Methods Results A cohort of 205 post-AMI patients (age 59.8±9.2, 92.2% male) was propensity-matched with CAD 60.5±10.0, 90.2% male). Coronary CT angiography non-contrast scans were performed to assess PVAT mean attenuation across...
In non-cancerous cells, transforming growth factor-β (TGFβ) regulates cellular responses primarily through Smad signaling. However, during cancer progression (including colorectal) TGFβ promotes tumoral via Smad-independent mechanisms and is involved in crosstalk with various pathways like the mitogen-activated protein kinases (MAPK) Wnt. Crosstalk between these following activation by subsequent downstream signaling activity can be referred to as a signature. This review highlights progress...
Acute myocardial infarction (AMI) is a leading cause of mortality and morbidity worldwide. Using validated efficient ICP-MS/MS-based workflow, total 30 metallomic features were profiled in study comprising 101 AMI patients 66 age-matched healthy controls. The include 12 essential elements (Ca, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, Zn), 8 non-essential/toxic (Al, As, Ba, Cd, Cr, Ni, Rb, Sr, U, V), 10 clinically relevant element-pair product/ratios (Ca/Mg, Ca×P, Cu/Se, Cu/Zn, Fe/Cu, P/Mg, Na/K,...
We recently discovered that Mfsd2b, which is the S1P exporter found in blood cells. Here, we report Mfsd2b critical for release of all species both resting and activated platelets. show platelets store cytoplasm. After activation, this pool delivered to plasma membrane, where predominantly localized export. Employing knockout mice reveal contribute a minor amount S1P. Nevertheless, deletion whole body or impairs platelet morphology functions. In particular, significantly reduced thrombus...
Background Because of a nonresponse to aspirin (aspirin resistance), patients with acute coronary syndrome (ACS) are at increased risk developing recurrent event. The in vitro platelet function tests have potential limitations, making them unsuitable for the detection resistance. We investigated whether miR-19b-1-5p could be utilized as biomarker resistance and future major adverse cardio-cerebrovascular (MACCE) events ACS. Methods Results In this cohort study, ACS were enrolled from...
Objective: While the risk of acute coronary events has been associated with biological variability circulating cholesterol, association other atherogenic lipids remains less understood. We evaluated longitudinal 284 and investigated their asymptomatic atherosclerosis. Approach Results: Circulating were extracted from fasting blood samples 83 community-sampled symptom-free participants (age 41–75 years), collected longitudinally over 6 months. Three types plaque volume (calcified, lipid-rich,...
Acute myocardial infarction (AMI) is a leading cause of mortality and morbidity worldwide. This work aims to investigate the translational potential multi-omics study (comprising metabolomics, lipidomics, glycomics, metallomics) in revealing biomechanistic insights into AMI. Following N-glycomics metallomics studies performed by our group previously, untargeted metabolomic lipidomic profiles were generated analysed this via use simultaneous metabolite/lipid extraction liquid...
Background The comparison of coronary computed tomography angiography plaques and perivascular adipose tissue (PVAT) between patients with acute myocardial infarction (AMI) posttreatment stable artery disease is poorly understood. Our objective was to evaluate the differences in angiography–quantified plaque PVAT characteristics post‐AMI identify signs residual inflammation. Methods Results We analyzed 205 (age, 59.77±9.24 years; 92.20% men) AMI ≤1 month matched them 60.52±10.04 90.24% based...
Patients suffering from acute myocardial infarction (AMI) are at risk of secondary outcomes including major adverse cardiovascular events (MACE) and heart failure (HF). Comprehensive molecular phenotyping cardiac imaging during the post-discharge time window may provide cues for stratification outcomes.In a prospective AMI cohort in New Zealand (N = 464), we measured plasma proteins lipids 30 days after hospital discharge inferred unified partial correlation network with echocardiographic...
Abstract Acute myocardial infarction (AMI) is a leading cause of mortality and morbidity worldwide. Diagnostic challenges remain in this highly time-sensitive condition. Using capillary electrophoresis-laser-induced fluorescence, we analyzed the blood plasma N-glycan profile cohort study comprising 103 patients with AMI 69 controls. Subsequently, data generated was subjected to classification modeling identify potential biomarkers. An area under Receiving Operating Characteristic curve...
Bioinformatics and machine learning tools have made it possible to integrate data across different -omics platforms for novel multiomic insights into diseases. To synergistically process in an integrative manner, analyte extractions each type need be done on the same set of clinical samples. Therefore, we introduce a simultaneous dual extraction method generating both metabolomic (polar metabolites only) glycomic (protein-derived N-glycans profiles from one sample with good efficiency...
Introduction Coagulation is involved in fibroproliferative responses following acute myocardial infarction (AMI). Left ventricular (LV) remodeling AMI closely associated with progression to heart failure. This study aims assess the association between plasma tissue factor activity and LV post-AMI patients. Methods We studied 228 patients 57 healthy subjects. Patients were categorized into two age- sex-matched groups: adverse or reverse remodeling, defined by an increase decrease,...
The relationship between trimethylamine-N-oxide (TMAO), betaine, and choline with acute myocardial infarction (AMI) end point remains unclear. We analyzed plasma TMAO, concentrations in AMI cases non-AMI community-dwelling controls by LC-MS/MS to understand how the balance these metabolites helps reduce risk. Results showed that odds ratio (OR) for highest versus lowest quartiles of betaine was 0.30 (95% CI, 0.10–0.82) after adjustment risk factors, unadjusted OR quartile 3 1 TMAO 2.47...
Abstract Acute myocardial infarction (AMI) is a leading cause of mortality and morbidity worldwide. Using validated efficient ICP-MS/MS-based workflow, total 30 metallomic features were profiled in study comprising 101 AMI patients 66 age-matched healthy controls. The include 12 essential elements (Ca, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, Zn), 8 non-essential/toxic (Al, As, Ba, Cd, Cr, Ni, Rb, Sr, U, V), 10 clinically relevant element-pair product/ratios (Ca/Mg, Ca×P, Cu/Se, Cu/Zn, Fe/Cu,...
The subset of plasma extracellular vesicles (EVs) that coprecipitate with low-density lipoprotein (LDL-EVs) carry coagulation and fibrinolysis pathway proteins as cargo. We investigated the association between LDL-EV hemostatic/fibrinolysis protein ratios post-acute myocardial infarction (post-AMI) left ventricular (LV) remodeling which precedes heart failure. Protein concentrations von Willebrand factor (VWF), SerpinC1 plasminogen were determined in LDL-EVs extracted from samples obtained...
Using the properties of universal approximation multilayer perceptron neural networks, a class discrete nonlinear dynamical systems are modeled by with two hidden layers. The authors' backpropagation algorithm is then used to train model identify desired degree accuracy. Based on identified model, one step ahead predictive control scheme proposed in which future inputs obtained through some optimization process. Making use online learning further developed into an adaptive robust...