A5070202825- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Nanoplatforms for cancer theranostics
- Signaling Pathways in Disease
- Cardiovascular Disease and Adiposity
- Neurobiology and Insect Physiology Research
- Atherosclerosis and Cardiovascular Diseases
- Wound Healing and Treatments
- Skin Protection and Aging
- Protease and Inhibitor Mechanisms
- Parasitic infections in humans and animals
- Gut microbiota and health
- Fatty Acid Research and Health
- Venomous Animal Envenomation and Studies
- Mesenchymal stem cell research
- Infant Nutrition and Health
- Cardiovascular Effects of Exercise
- Cardiac Ischemia and Reperfusion
- Mechanical Circulatory Support Devices
- Amoebic Infections and Treatments
- Insect and Pesticide Research
- Advancements in Transdermal Drug Delivery
South China University of Technology
2025
National University of Singapore
2019-2024
National University Heart Centre Singapore
2019-2023
Milk-derived extracellular vesicles (mEVs) have been proposed as a potential nanomedicine for intestinal disorders; however, their impact on barrier integrity in gut inflammation and associated metabolic diseases has not explored yet. Here, mEVs derived from bovine human breast milk exert similar protective effects epithelial tight junction functionality vitro, survive harsh gastrointestinal conditions ex vivo, reach the colon vivo. Oral administration of restores at multiple levels,...
Wound healing is a dynamic process that involves series of molecular and cellular events aimed at replacing devitalized missing components and/or tissue layers. Recently, extracellular vesicles (EVs), naturally cell-secreted lipid membrane-bound laden with biological cargos including proteins, lipids, nucleic acids, have drawn wide attention due to their ability promote wound regeneration. However, current exploitation EVs as therapeutic agents limited by low isolation yields tedious...
Atherosclerosis is the primary cause of cardiovascular events such as heart attacks and strokes. However, current medical practice lacks non-invasive, reliable approaches for both imaging atherosclerotic plaques delivering therapeutic agents directly therein. Here, a biocompatible biodegradable pH-responsive nanoscale coordination polymers (NCPs) based theranostic system reported managing atherosclerosis. NCPs are synthesized with benzoic-imine (BI) linker Gd
The coagulation protein tissue factor (TF) regulates inflammation and angiogenesis via its cytoplasmic domain in infection, cancer diabetes. While TF is highly abundant the heart implicated cardiac pathology, contribution of to post-infarct myocardial injury adverse left ventricular (LV) remodeling remains unknown. Methods: Myocardial infarction was induced wild-type mice or lacking (TF∆CT) by occlusion anterior descending coronary artery. Heart function monitored with echocardiography....
Recently, bioinspired cell-derived nanovesicles (CDNs) have gained much interest in the field of nanomedicine due to preservation biomolecular structure characteristics derived from their parent cells, which impart CDNs with unique properties terms binding and uptake by target cells intrinsic biological activities. Although production can be easily reproducibly achieved any kind cell culture, application for therapeutic purposes has been greatly hampered physical chemical instability during...
In drug delivery, the development of nanovesicles that combine both synthetic and cellular components provides added biocompatibility targeting specificity in comparison to conventional carriers such as liposomes. Produced through fusion U937 monocytes’ membranes lipids, our nano-cell vesicle technology systems (nCVTs) showed promising results targeted cancer treatment. However, no investigation has been conducted yet on immunogenic profile uptake mechanisms nCVTs. Hence, this study was...
Extracellular vesicles (EVs), which are miniaturised carriers loaded with functional proteins, lipids, and nucleic acid material, naturally secreted by cells show intrinsic pharmacological effects in several conditions. As such, they have the potential to be used for treatment of various human diseases. However, low isolation yield laborious purification process obstacles their translation clinical use. To overcome this problem, our lab developed cell-derived nanovesicles (CDNs), EV mimetics...
Involvement of the Toll-like receptor 4 (TLR4) in maladaptive cardiac remodeling and heart failure (HF) upon pressure overload has been studied extensively, but less is known about role TLR2. Interplay redundancy TLR4 with TLR2 have reported other organs were not investigated during dysfunction. We explored whether deficiency leads to adverse chronic additively contribute this. subjected 35 male C57BL/6J mice (wildtype (WT) or knockout (KO)) sham transverse aortic constriction (TAC) surgery....
Abstract Background Accounting for around 50% of human heart failure, failure with preserved ejection fraction (HFpEF) is driven by complex and heterogeneous pathological factors, including gut microbiome dysbiosis. We have previously reported that milk-derived extracellular vesicles (mEVs) modulate the microbiota in inflammatory bowel disease. However, whether mEVs can affect thereby alleviate HFpEF remains unexplored. Aims aim to uncover potential therapeutic effects on if so, underlying...
The subset of plasma extracellular vesicles (EVs) that coprecipitate with low-density lipoprotein (LDL-EVs) carry coagulation and fibrinolysis pathway proteins as cargo. We investigated the association between LDL-EV hemostatic/fibrinolysis protein ratios post-acute myocardial infarction (post-AMI) left ventricular (LV) remodeling which precedes heart failure. Protein concentrations von Willebrand factor (VWF), SerpinC1 plasminogen were determined in LDL-EVs extracted from samples obtained...
Abstract Introduction The coagulation protein tissue factor (TF) regulates inflammation and angiogenesis via its cytoplasmic domain in infection, cancer diabetes. While TF is highly abundant the heart implicated cardiac injuries dysfunction, contribution of pathology remains unclear. Purpose We aimed to investigate post-infarct myocardial injury adverse left ventricular (LV) remodeling. Methods results Myocardial infarction was induced by permanent occlusion anterior descending coronary...
Blastocystis is a genus of single-celled protist belonging to the stramenopile group. Prior studies have shown that isolates subtype 7 (ST7) induced higher levels intestinal epithelial cell damage and gut microbiota dysbiosis in comparison other subtypes vivo vitro settings. research has link between exposure extracellular vesicles (EVs) produced by pathogenic microorganisms. This study demonstrates protocol for isolation EVs from ST7 via ultracentrifugation. Nanoparticle tracking analysis...