A5100711946- Cardiovascular Function and Risk Factors
- Sepsis Diagnosis and Treatment
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Heart Failure Treatment and Management
- Venous Thromboembolism Diagnosis and Management
- Cardiac Imaging and Diagnostics
- Fibroblast Growth Factor Research
- Cardiac pacing and defibrillation studies
- Cardiac Fibrosis and Remodeling
- Kruppel-like factors research
- Intensive Care Unit Cognitive Disorders
- Connective Tissue Growth Factor Research
- Cardiac Arrhythmias and Treatments
- Acute Myocardial Infarction Research
- Healthcare and Venom Research
- Apelin-related biomedical research
- Pancreatic and Hepatic Oncology Research
- Advanced Clustering Algorithms Research
- Vacuum and Plasma Arcs
- ECG Monitoring and Analysis
- Scoliosis diagnosis and treatment
- Marine and coastal plant biology
Shanghai Medical College of Fudan University
2023-2024
Fudan University Shanghai Cancer Center
2023-2024
Hubei Academy of Agricultural Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Hubei University of Technology
2024
Chinese PLA General Hospital
2019-2023
Hebei Medical University
2023
Guangzhou University of Chinese Medicine
2022-2023
Second Hospital of Hebei Medical University
2023
National University of Singapore
2023
Clinically, activated EGFR mutation associated chemo-drugs resistance has severely threaten NSCLC patients. Nanoparticle based small interfering RNA (siRNA) therapy representing another promising alternative by silencing specific gene while still suffered from charge toxicity, strong immunogenicity and poor targetability. Herein, we reported a novel EGFR-mutant relying on edible cation-free kiwi-derived extracellular vesicles (KEVs), which showed sevenfold enhancement of safe dosage compared...
Sepsis-associated encephalopathy (SAE) is a critical and common problem in elderly patients with sepsis, which still short of efficient predictive tools. Therefore, this study aims to screen the risk factors establish useful nomogram for SAE sepsis intensive care unit (ICU).Elderly (age ≥ 65 years) were selected from Medical Information Mart Intensive Care (MIMIC)-IV database. Data demographics laboratory examinations collected on first day admission ICU. was defined by two criteria presence...
MARCH8, an E3 ubiquitin ligase, plays a crucial role in regulating various cellular processes such as protein degradation and signaling pathways is implicated the development spread of pancreatic cancer. Analysis cancer tissues compared to adjacent normal showed decrease miRNA-30d-5p levels increase OIP5-AS1 MARCH8 levels, confirmed by qRT-PCR Western blot analysis. The dual-luciferase reporter assay demonstrated binding relationship between miRNA-30d-5p, well PACN-1 cells, derived from...
FcαR (CD89) is the Fc receptor for immunoglobulin A (IgA) and plays important roles in IgA-mediated immune responses. It a heavily glycosylated protein with six potential N-linked glycosylation sites. Previous reports showed that abnormal of was involved some diseases, including human immunodeficiency virus infection, alcoholic liver cirrhosis IgA nephropathy. In this study, we examined effects N-glycosylation on interaction between IgA. We found depletion transfected Chinese hamster ovary...
Extracellular vesicles (EVs) are nano-sized membrane released by various cell types. Mammalian EVs have been studied in-depth, but the role of plant has rarely explored. For first time, from Drynariae Rhizoma roots were isolated and identified using transmission electron microscopy a flow nano analyzer. Proteomics bioinformatics applied to determine protein composition complete functional analysis EVs. Seventy-seven proteins root-derived EVs, with enzymes accounting for 47% proteins. All...
The role E3 ubiquitin ligase membrane-associated RING-CH 8 (MARCH8) has not been studied in pancreatic cancer.Pancreatic cancer cell lines and the normal cells were tested vitro studies male athymic nude mice vivo studies. Measuring viability by Cell Counting Kit-8 assay (CCK8), 5-ethynyl-2'- deoxyuridine (Edu) staining, colony formation assay. Wound healing was implemented for migration Transwell performed invasion to evaluate histological status hematoxylin eosin staining detect protein...
Patients suffering from acute myocardial infarction (AMI) are at risk of secondary outcomes including major adverse cardiovascular events (MACE) and heart failure (HF). Comprehensive molecular phenotyping cardiac imaging during the post-discharge time window may provide cues for stratification outcomes.In a prospective AMI cohort in New Zealand (N = 464), we measured plasma proteins lipids 30 days after hospital discharge inferred unified partial correlation network with echocardiographic...
In biological network analysis, the goal of target identification problem is to predict molecule inhibit (or activate) achieve optimum efficacy and safety for a disease treatment. A related prioritization which predicts subset molecules in given disease-related contains successful drug targets with highest probability. Sensitivity analysis prioritizes dynamic model using principled criteria, but fails penalize off-target effects, does not scale large networks. We describe Pani (Putative...
Abstract Background Atrial fibrosis has been recognized as a typical pathological change in atrial fibrillation. Although present evidence suggests that microRNA-499-5p (miR-499-5p) plays an important role the development of fibrosis, specific mechanism is not fully understood. Therefore, this study attempted to assess influence miR-499-5p on fibroblasts and explore potential molecular mechanism. Methods from sprague dawley rat were respectively transfected with mimic, negative control...
Immunological detection of viruses and their components using monoclonal antibodies (MAbs) is a powerful diagnostic method. Here we report detailed method for the establishment MAbs against severe acute respiratory syndrome coronavirus (SARS-CoV). To express purify nucleocapsid protein (N protein) SARS-CoV generate N protein, gene encoding was separated into two parts according to prediction epitopes cloned pET32a(+), respectively. Expression target proteins were induced by M...
Purpose: This study evaluated the association among plasma concentration of ticagrelor, ARC124910XX, aspirin, and salicylic acid with risk recent bleeding in patients acute coronary syndrome. To this end, we developed an accurate model to predict bleeding. Methods: A total 84 included cohort between May 2021 November 2021. The factors were identified by univariate multivariate analyses, statistically significant analysis nomogram. We used calibration curve receiver operating characteristic...
We investigated the clinical value of Doppler echocardiography for determining optimal setting atrioventricular interval in patients with dual chamber pacemakers (DDD). In 38 complete block and DDD pacemakers, was prolonged a stepwise fashion by 20 ms, from 90 to 250 cardiac stroke volume transmitral flow were measured pulsed echocardiography.The at which maximal 168.9 +/- 15.6 ms. The 178.4 23.4 ms when end A wave coincided closure mitral valve.There significant linear relationship between...
Recent functional genomics research has yielded a large in silico gene regulatory network model (622 nodes) for endomesoderm development of sea urchin, organism embryonic development. The size this makes it challenging to determine which genes are most responsible given biological effect. In paper, we explore feasibility and accuracy existing techniques identifying key that regulate Endo16, widely-accepted gastrulation marker. We apply target prioritization tools (sensitivity analysis PANI)...