A5037076747- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- Muscle metabolism and nutrition
- ATP Synthase and ATPases Research
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Tendon Structure and Treatment
- Cardiovascular Disease and Adiposity
- Cardiac Fibrosis and Remodeling
- Adipose Tissue and Metabolism
- Histone Deacetylase Inhibitors Research
- Machine Learning in Bioinformatics
- Bee Products Chemical Analysis
- Cardiomyopathy and Myosin Studies
- Genetics and Neurodevelopmental Disorders
- Cardiac Ischemia and Reperfusion
- Exercise and Physiological Responses
- Computational Drug Discovery Methods
- Protease and Inhibitor Mechanisms
- Liver Disease Diagnosis and Treatment
Tsinghua University
2025
Center for Life Sciences
2025
University of California, Los Angeles
2011-2024
New York University
2015-2024
Chongqing Medical University
2011-2021
Second Affiliated Hospital of Chongqing Medical University
2011-2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Dalian Medical University
2011-2021
Institute of Hematology & Blood Diseases Hospital
2021
National Clinical Research
2021
Mitochondrial dysfunction is associated with many human diseases. damage exacerbated by inadequate protein quality control and often further contributes to pathogenesis. The maintenance of mitochondrial functions requires a delicate balance continuous synthesis degradation, i.e. turnover. To understand dynamics in vivo, we designed metabolic heavy water ((2)H(2)O) labeling strategy customized examine individual turnover the mitochondria systematic fashion. Mice were fed (2)H(2)O at minimal...
Protein temporal dynamics play a critical role in time-dimensional pathophysiological processes, including the gradual cardiac remodeling that occurs early-stage heart failure. Methods for quantitative assessments of protein kinetics are lacking, and despite knowledge gained from single-protein studies, integrative views coordinated behavior multiple proteins scarce. Here, we developed workflow integrates deuterium oxide (2H2O) labeling, high-resolution mass spectrometry (MS), custom...
Abstract Protein stability is a major regulatory principle of protein function and cellular homeostasis. Despite limited understanding on mechanisms, disruption turnover widely implicated in diverse pathologies from heart failure to neurodegenerations. Information global dynamics therefore has the potential expand depth scope disease phenotyping therapeutic strategies. Using an integrated platform metabolic labeling, high-resolution mass spectrometry computational analysis, we report here...
Amino acid deprivation promotes the inhibition of kinase complex mTORC1 (mammalian target rapamycin 1) and activation GCN2 (general control nonrepressed 2). Signaling pathways downstream both kinases have been thought to independently induce autophagy. We showed that these two amino acid-sensing systems are linked. pharmacological led phosphorylation eukaryotic initiation factor 2α (eIF2α) in a mechanism dependent on catalytic subunit protein phosphatase 6 (PP6C). Autophagy induced by...
Nitric oxide (NO) inhibits transport in various nephron segments, and the thick ascending limb of loop Henle (TALH) expresses NO synthase (NOS). However, effects on TALH have not been extensively studied. We hypothesized that endogenously produced directly decreases NaCl by TALH. first determined effect exogenously added net chloride flux ( J Cl ). The donor spermine NONOate (SPM; 10 μM) decreased from 101.2 ± 9.6 to 65.0 7.7 pmol ⋅ mm −1 min , a reduction 35.5 6.4%, whereas controls did...
Proteasome complexes play essential roles in maintaining cellular protein homeostasis and serve fundamental cardiac function under normal pathological conditions. A functional detriment proteasomal activities has been recognized as a major contributor to the progression of cardiovascular diseases. Therefore, approaches restore proteolytic within setting diseased myocardium would be great clinical significance. In this study, we discovered that activity could regulated by acetylation. Histone...
Purpose High‐throughput quantification of human protein turnover via in vivo administration deuterium oxide ( 2 H O) is a powerful new approach to examine potential disease mechanisms. Its immediate clinical translation contingent upon characterizations the safety and hemodynamic effects O subjects. Experimental design We recruited ten healthy subjects with broad demographic variety evaluate safety, feasibility, efficacy, reproducibility intake for studying dynamics. designed protocol where...
Purpose: A key factor in regulating bone absorption is the proportion of RANKL/OPG. Although many reports showing diverse transcription factors or epigenetic modification could be responsible for RANKL&OPG ratio, there still little exploration on promoter methylation status both genes osteoporotic tissues. Our aim to investigate changes CpG island these genes' promoters patients with primary osteoporosis. Methods: The diagnosis osteoporosis was based results dual energy X-ray absorptiometry...
Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover repair damaged tissue. However, proteome dynamics remain largely undefined, as do differences in rates individual proteins the collagen and glycoprotein phases extracellular matrix (ECM). Here, we investigate tissues by exploiting spatially distinct fascicular (collagen-rich) interfascicular (glycoprotein-rich) ECM tendon. Using isotope labelling, mass spectrometry bioinformatics,...
The thick ascending limb of the loop Henle (THAL) plays an essential role in regulation sodium and water homeostasis by kidney. l-Arginine, substrate for nitric oxide synthase (NOS), decreases NaCl absorption THALs. We hypothesized that eNOS produces NO regulates THAL transport selective expression knockout(-/-) mice would restore effects l-arginine on absorption. eNOS-/- were anesthetized, left kidney was exposed, renal interstitium injected with recombinant adenoviral vectors expressed...
Abstract We describe a method for the simultaneous extraction of proteins and nucleic acids from Medicago truncatula tissues. Using modified TRIzol reagent method, we developed simple an effective way to simultaneously extract single sample. verified that this does not affect quality or quantitation isolated DNA RNA. Furthermore, used 2‐DE compare M. leaf, stem, root samples processed using new with two commonly methods: phenol extraction/methanol–ammonium acetate precipitation...
Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, physiological and phenotypic changes induced salt treatments (10-50 mM NaCl Na2SO4 mixtures) were analysed Zhongmu-3 (Medicago sativa) R108 truncatula) seedlings. Our observations indicated that was more salt-tolerant than R108. To characterize protein expression profiles these two Medicago species in response to...
Rationale: Mitochondrial protein homeostasis is an essential component of the functions and oxidative stress responses heart. Objective: To determine specificity efficiency proteome turnover cardiac mitochondria by endogenous exogenous proteolytic mechanisms. Methods Results: Proteolytic degradation murine was assessed 2-dimensional differential gel electrophoresis liquid chromatography–tandem mass spectrometry. proteases demonstrated a substrate preference for basic variants, which...
The direct transduction of a peptide cleavage event into DNA detection has always produced output with some amino acid residues, which influence the amplification efficiency in view their steric hindrance effect. Here an ingenious two-stage template was designed to achieve highly efficient by utilizing exponential reaction (EXPAR) as model. usage not only accomplished traditionally inefficient EXPAR triggered residues but also simultaneously newly identical trigger without any induce extra...
Many studies have reported that BRCA1 polymorphisms are associated with cancer risk, but the results remain controversial. The purpose of this meta-analysis is to evaluate relationship between (rs799917, rs1799950, rs1799966, or rs16941) and risk. Relevant were identified via a systematic search PubMed, Embase, Web Science databases up July 31, 2017. Odds ratios (ORs) 95% confidence intervals (CIs) calculated examine strength associations. Thirty-five published in 19 publications involving...
Cysteine oxidative modification of cellular proteins is crucial for many aspects cardiac hypertrophy development. However, integrated dissection multiple types cysteine post-translational modifications (O-PTM) proteomes in currently missing. Here we developed a novel discovery platform that encompasses customized biotin switch-based quantitative proteomics pipeline and an advanced analytic workflow to comprehensively profile the landscape O-PTM ISO-induced mouse model. Specifically,...
Altered proteasome functions are associated with multiple cardiomyopathies. While the targets polyubiquitinated proteins for destruction, it itself is modifiable by ubiquitination. We aim to identify exact ubiquitination sites on cardiac proteasomes and examine whether they also subject acetylations.Assembled 20S complexes were purified from five human hearts ischemic cardiomyopathy, then analyzed high-resolution MS acetylation sites. developed a library search strategy that may be used...
A growing body of biomedical literature suggests a bidirectional regulatory relationship between cardiac calcium (Ca