A5103149795- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- Medicinal Plant Pharmacodynamics Research
- Circular RNAs in diseases
- Bone health and osteoporosis research
- MicroRNA in disease regulation
- Bone health and treatments
- RNA Research and Splicing
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- RNA modifications and cancer
- Lipid metabolism and disorders
- Epigenetics and DNA Methylation
- Bone fractures and treatments
- Wnt/β-catenin signaling in development and cancer
Second Affiliated Hospital of Guangzhou Medical University
2016-2023
Guangzhou Medical University
2016-2023
Recent studies have shown that long non-coding RNAs (lncRNAs) critical roles in tumorigenesis, including osteosarcoma. The lncRNA taurine-upregulated gene 1 (TUG1) was reported to be involved the progression of Here, we investigated role TUG1 osteosarcoma cells and underlying mechanism. expression measured cell lines human normal osteoblast by quantitative real-time PCR (qRT-PCR). effects on were studied RNA interference vitro vivo. mechanism competing endogenous (ceRNA) determined using...
Purpose: A key factor in regulating bone absorption is the proportion of RANKL/OPG. Although many reports showing diverse transcription factors or epigenetic modification could be responsible for RANKL&OPG ratio, there still little exploration on promoter methylation status both genes osteoporotic tissues. Our aim to investigate changes CpG island these genes' promoters patients with primary osteoporosis. Methods: The diagnosis osteoporosis was based results dual energy X-ray absorptiometry...
Osteoporosis (OP) is a metabolic disease characterized by decreased bone mass and increased risk of fragility fractures, which significantly reduces the quality life. Stem cell-based therapies, especially using marrow mesenchymal stem cells (BMSCs), are promising strategy for treating OP. Nevertheless, survival differentiation rates transplanted BMSCs low, limits their therapeutic efficiency. Icariin (ICA) traditional Chinese medicine formulation that prescribed tonifying kidneys. It also...
Icaritin, a metabolite of icariin, is potent promoter bone marrow‑derived mesenchymal stem cells (BMSCs) osteogenesis, but the underlying mechanisms remain unclear. To examine effects icaritin on osteogenic differentiation, BMSCs were exposed to induction medium with or without pretreatment in present study. It was identified that (0.01‑1 µM) exhibited no cytotoxicity proliferative abilities BMSCs. Icaritin at 1 µM increased alkaline phosphatase activity, mineral deposition and...
Purpose . SOST gene is one of the key factors in regulating bone absorption. Although there are reports showing diverse transcription factors, epigenetic modification could be responsible for expression. There still little exploration on promoter methylation status osteoporotic tissues. The aim this study to investigate involvement CpG regulation expression patients with primary osteoporosis. Methods diagnosis osteoporosis was established basis dual energy X-ray absorptiometry measure BMD....
Osteosarcoma (OS) is a primary malignant tumor with complex etiology. Therefore, research into the pathogenesis of osteosarcoma considered priority. Circular RNAs play important roles in cell metabolism and immune response are closely associated cancer treatment. However, association circular limited. In present study, CircSAMD4A was validated by RT‑qPCR agarose gel electrophoresis. miR‑342‑3p expression detected RT‑qPCR. The relative protein levels were measured western blot analysis. MTT...
miR-218 is associated with osteogenesis. Bioinformatics analysis showed a targeting relationship between and GSK-3 β 3′-UTR. Our study assessed whether regulates expression affects osteoblast differentiation of bone marrow mesenchymal stem cells (BMSCs). Osteogenic induction medium was used to induce BMSCs differentiate into osteoblasts. miR-218, , -catenin RUNX2 level detected during D10 D20 differentiation. were divided antagomir-NC antagomir-218, induced for 20 days followed by level,...
Abstract Objective Detect and analyse the correlation between factors of related pathways (Wnt/β-catenin, BMP-2/Runx2/Osterix, OPG/RANKL LGR4/RANKL) postmenopausal osteoporotic fracture (PMOPF). Methods The patients with tibial were divided into control group (36 cases) PMOPF cases). Using RNAiso Plus method to extract total RNA bone tissue, RT-qPCR was used detect expression each factor. Detected levels serum by ELISA in group. A-F according blood collection time interval (at different...
Abstract Objective: To investigate the variations in expressions of LRP5, Runx2, Osterix, and RANKL factors bone tissues associated with postmenopausal osteoporotic fractures (PMOPF). Method: Postmenopausal patients femur were initially divided into control (31 cases) PMOPF groups (83 cases). All group operated within 1 day after injury. The based on time fracture respective (patients A, B, C fracture). Samples collected from femurs at sites during operation. expression level each factor was...