A5101625037- Hepatitis B Virus Studies
- Hepatitis C virus research
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Liver Disease Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Interference and Gene Delivery
- S100 Proteins and Annexins
- HIV Research and Treatment
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- interferon and immune responses
- Disaster Response and Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- Sepsis Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Systemic Lupus Erythematosus Research
- Viral gastroenteritis research and epidemiology
- Rheumatoid Arthritis Research and Therapies
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Adenosine and Purinergic Signaling
- Hepatitis Viruses Studies and Epidemiology
Second Affiliated Hospital of Chongqing Medical University
2013-2025
Chongqing Medical University
2013-2025
Dalian Medical University
2013-2025
Guangxi Medical University
2022
First People's Hospital of Chongqing
2021
Chongqing University
2007
Neutrophil extracellular traps (NETs) are considered significant contributors to cancer progression, especially metastasis. However, it is still unclear whether NETs involved in hepatitis B virus (HBV)-related hepatocarcinogenesis and have potential clinical significance during evaluation management for hepatocellular carcinoma (HCC). In this study, we aimed investigate the functional mechanism of HBV-related their significance. A total 175 HCC patients with without HBV infection 58 healthy...
The mechanism underlying HCC metastasis remains unclear, many oncogenes are known to regulate this process. However, the role of alternative splicing (AS) in pro-metastatic is poorly understood.By performing RNA sequencing on nine pairs primary tissues with extrahepatic (EHMH) and metastasis-free (MFH) tissues, we depicted AS landscape found a higher frequency events EHMH compared MFH. Moreover, 28 differentially expressed regulators were identified Among these, DEAD-box helicase 17 (DDX17)...
Enterobacter cloacae is increasingly prevalent and resistant to multiple antibiotics, making it a significant pathogen in healthcare settings with high mortality rates. However, its pathogenic mechanisms are not fully understood. In this study, we explored the role of nagZ regulating virulence E. potential as therapeutic target. Our research showed that strains express higher levels than colonizing strains, particularly simulated infection environments. Deleting significantly reduced various...
Abstract Cancer stem cells exhibit flexible metabolic profiles. However, the underlying mechanisms for differential pathways affecting stemness maintenance in gastric cancer are poorly understood. Here, we reveal role of serine hydroxymethyltransferase‐2 (SHMT2)/serine‐mediated crosstalk between one‐carbon metabolism and lipid cancer. Clinically, SHMT2 was significantly highly expressed Gastric (GCs) cells, associated with clinical malignant features poor prognosis patients. Mechanistically,...
Abstract Background Cyclic GMP-AMP synthase (cGAS) is a crucial DNA sensor and plays an important role in host antiviral innate immune responses. During hepatitis B virus (HBV) infection, the cGAS signaling pathway can suppress HBV replication. As regulatory protein of HBV, X (HBx) may serve as antagonistic character to cGAS/STING pathway. In this study, we aim investigate functional HBx Methods The effects on IFN-β promoter activity were measured by Dual-luciferase reporter assays....
Some reports revealed that rapamycin could reactivate HBV infection. However, the mechanism has not been clearly explained.In this report, we studied by which enhances replication and expression inducing cellular autophagy.HepG2.2.15 cells were treated with to induce autophagy. Autophagosomes observed fluorescence microscopy transmission electron microscopy. Autophagy marker protein LC3-Ⅱ/LC3-Ⅰwas detected Western blotting. DNA mRNA determined real time PCR Southern HBsAg was evaluated...
Abstract Hepatitis B virus (HBV) vaccination was considered the most powerful tool for prevention of HBV transmission from mother to infant. In 1992, a universal program infants launched in China. The aim this study investigate surface antigen (HBsAg) variations immunoprophylaxis‐failed infants, vaccine free infant patients and adult chronic hepatitis (CHB) (without nucleoside analog treatment). Immunoprophylaxis‐failed were recruited two centers (Guangdong Chongqing, representing Southern...
Substantial advance supports that CMTM2 serve as an important performer in physiological and pathological processes. However, very little is clear about the relationship between HBV-related disorders. Here, for first time, we explore whether or not serum involved diseases. We found values were significantly lower patients compared to healthy control (p <0.001), using ELISA assay. Furthermore, levels negatively correlated with HBV DNA CHB but of ALT AST. Serum concentrations ALT, AST load...
Immunological detection of viruses and their components using monoclonal antibodies (MAbs) is a powerful diagnostic method. Here we report detailed method for the establishment MAbs against severe acute respiratory syndrome coronavirus (SARS-CoV). To express purify nucleocapsid protein (N protein) SARS-CoV generate N protein, gene encoding was separated into two parts according to prediction epitopes cloned pET32a(+), respectively. Expression target proteins were induced by M...
To demonstrate whether procalcitonin (PCT) combined with calcitonin (CT) could provide additional diagnostic value to other clinically available rheumatoid arthritis- (RA-) related biomarkers in the early diagnosis of RA. The blood samples aseptically collected by venipuncture were centrifuged within 1 hour and frozen at -80°C. PCT CT levels measured using electrochemiluminescence immunoassay (ECLIA) 260 subjects (48 patients RA, 34 established 37 systemic lupus erythematosus, 30...
PreS1 is a hypothetical candidate domain of L protein for hepatitis B virus (HBV) to adhere and invade host hepatic cells. This report deals with the expression purification recombinant adw2 subtype preS1 peptide surface antigen in Escherichia coli. The DNA fragments full-length or N/C terminal sequence synthesized by PCR were inserted into prokaryotic vector pGST-MOLUC, respectively. Reconstitute plasmids (named pGST-preS1, pGST-preS1N, pGST-preS1C) confirmed sequencing analysis transferred...
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Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough cells effectively.
Objective: To investigate the effect of zinc finger E-box-binding homeobox 2 (ZEB2) on hepatitis B virus (HBV) replication and expression. Methods: HepG2, HepG2.2.15, HepAD38 cells were cultured separately, Western blot was used to measure expression ZEB2. HepG2.2.15 transfected with ZEB2 plasmids or shRNA targeting HBV core proteins, quantitative real-time PCR 3.5 kb RNA DNA, Southern replicative intermediate, ELISA HBsAg HBeAg, in order clarify The dual-luciferase reporter system analyze...