A5033320013- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Polysaccharides and Plant Cell Walls
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Enzyme Catalysis and Immobilization
- Cancer Research and Treatments
- bioluminescence and chemiluminescence research
- Bacterial Genetics and Biotechnology
- Advanced Cellulose Research Studies
- Amino Acid Enzymes and Metabolism
- Microbial Metabolites in Food Biotechnology
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Microbial Natural Products and Biosynthesis
- Proteoglycans and glycosaminoglycans research
- Cancer-related gene regulation
- Toxoplasma gondii Research Studies
- Plant Reproductive Biology
- Biochemical and Structural Characterization
National Renewable Energy Laboratory
2014-2024
CoorsTek (United States)
2024
Ministry of Health of the Russian Federation
2019-2023
Medical Radiological Research Center
2023
P.A. Hertzen Moscow Oncology Research Institute
2020-2021
Bioengineering Center
2014
National Center for Genetic Engineering and Biotechnology
2014
John Wiley & Sons (United States)
2014
Agrobioinstitute
2012
Oak Ridge National Laboratory
2012
Most fungi and bacteria degrade plant cell walls by secreting free, complementary enzymes that hydrolyze cellulose; however, some use large enzymatic assemblies called cellulosomes, which recruit to protein scaffolds. The thermophilic bacterium Caldicellulosiruptor bescii uses an intermediate strategy, many free cellulases contain multiple catalytic domains. One of these, CelA, comprises a glycoside hydrolase family 9 48 domain, as well three type III cellulose-binding modules. In the...
SET domain methyltransferases deposit methyl marks on specific histone tail lysine residues and play a major role in epigenetic regulation of gene transcription. We solved the structures catalytic domains GLP, G9a, Suv39H2 PRDM2, four eight known human H3K9 their apo conformation or complex with donating cofactor, peptide substrates. analyzed structural determinants for methylation state specificity, designed G9a mutant able to tri-methylate H3K9. show that I-SET acts as rigid docking...
Abstract Glycoside Hydrolase Family 7 cellobiohydrolases (GH7 CBHs) catalyze cellulose depolymerization in cellulolytic eukaryotes, making them key discovery and engineering targets. However, there remains a lack of robust structure–activity relationships for these industrially important cellulases. Here, we compare CBHs from Trichoderma reesei ( Tr Cel7A) Penicillium funiculosum Pf Cel7A), which exhibit multi-modular architecture consisting catalytic domain (CD), carbohydrate-binding...
Metal-halide perovskite semiconductors have attracted attention for opto-spintronic applications where the manipulation of charge and spin degrees freedom potential to lower power consumption achieve faster switching times electronic devices. Lower-dimensional perovskites are particular interest since degree symmetry metal-halide connected octahedra large spin-orbit coupling can potentially lift degeneracy. To their full application potential, long spin-polarized lifetimes an understanding...
Helicobacter pylori flagellin is heavily glycosylated with the novel sialic acid-like nonulosonate, pseudaminic acid (Pse). The glycosylation process essential for assembly of functional flagellar filaments and consequent bacterial motility. Because motility a key virulence factor this other important pathogens, Pse biosynthetic pathway offers potential therapeutic targets. From recent NMR analyses, we determined that conversion UDP-alpha-D-Glc-NAc to central intermediate in pathway,...
The Escherichia coli isocitrate lyase regulator (IclR) regulates the expression of glyoxylate bypass operon (aceBAK). Founding member a large family common fold transcriptional regulators, IclR comprises DNA binding domain that interacts with operator sequence and C-terminal (C-IclR) binds hitherto unknown small molecule. We screened chemical library more than 150 metabolic scaffolds using high-throughput protein stability assay to identify molecules bind then tested active compounds in...
The mechanistic underpinnings of the complex process plant polysaccharide biosynthesis are poorly understood, largely because resistance glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes regiospecific transfer terminal 1,2-fucosyl residues xyloglucan side chains - key step in fucosylated sidechains galactoxyloglucan. We unravel basis for fucosylation by AtFUT1 with...
Auxiliary activity (AA) enzymes are produced by numerous bacterial and fungal species to assist in the degradation of biomass. These abundant but have yet be fully characterized. Here, we report X-ray structure Thermobifida fusca AA10A (TfAA10A), investigate mutational characterization key surface residues near its active site, explore importance various domains AA10B (TfAA10B). The TfAA10A is similar other LPMOs (lytic polysaccharide monooxygenases), including signs photo-reduction a...
Xylans are a major component of plant cell walls. O-Acetyl moieties the dominant backbone substituents glucuronoxylan in dicots and play role polymer-polymer interactions that crucial for wall architecture normal development. Here, we describe biochemical, structural, mechanistic characterization Arabidopsis (Arabidopsis thaliana) xylan O-acetyltransferase 1 (XOAT1), member plant-specific Trichome Birefringence Like (TBL) family. Detailed XOAT1-catalyzed reactions by real-time NMR confirms...
The biological and functional significance of selected Critical Assessment Techniques for Protein Structure Prediction 14 (CASP14) targets are described by the authors structures. highlight most relevant features target proteins discuss how well these were reproduced in respective submitted predictions. overall ability to predict three-dimensional structures has improved remarkably CASP14, many difficult modeled with impressive accuracy. For first time history CASP, experimentalists not only...
Protein engineering and screening of processive fungal cellobiohydrolases (CBHs) remain challenging due to limited expression hosts, synergy-dependency, recalcitrant substrates. In particular, glycoside hydrolase family 7 (GH7) CBHs are critically important for the bioeconomy typically difficult engineer. Here, we target discovery highly active natural GH7 variants with improved activity. Using experimentally assayed activities genome mined CBHs, applied sequence structural alignments top...
ABSTRACT Intracellular glucose in Escherichia coli cells imported by phosphoenolpyruvate-dependent phosphotransferase system-independent uptake is phosphorylated glucokinase using ATP to yield glucose-6-phosphate. Glucokinases (EC 2.7.1.2) are functionally distinct from hexokinases 2.7.1.1) with respect their narrow specificity for as a substrate. While structural information available ADP-dependent glucokinases Archaea , no exists the large sequence family of eubacterial ATP-dependent...
Members of the aminoacylase-1 (Acy1)/M20 family aminoacylases and exopeptidases exist as either monomers or homodimers. They contain a zinc-binding domain second mediating dimerization in latter case. The roles that both domains play catalysis have been investigated for human Acy1 (hAcy1) by x-ray crystallography site-directed mutagenesis. Structure comparison dinuclear zinc center mutant hAcy1 reported here with dizinc centers related enzymes points to difference ligation Acy1/M20 family....
The Pseudomonas aeruginosa PsrA autorepressor has dual roles as a repressor of the fadBA5beta-oxidation operon and an activator stationary-phase sigma factor rpoS exsCEBA type III secretion system (TTSS). Previously, we demonstrated that repression fadBA5 by is relieved long-chain fatty acids (LCFAs). However, signal affecting activation exsC via unknown. In this study, microarray gene fusion data suggested LCFA (e.g. oleate) affected expression exsC. DNA binding studies confirmed binds to...
Currently, the cost of cellulase enzymes remains a key economic impediment to commercialization biofuels. Enzymes from glycoside hydrolase family 48 (GH48) are critical component numerous natural lignocellulose-degrading systems. Although computational mining large genomic data sets is promising new approach for identifying novel cellulolytic activities, current methods unable distinguish between cellulases and with different substrate specificities that belong same protein family. We show...
Lignocellulosic biomass is a potential source of sustainable transportation fuels, but efficient enzymatic saccharification cellulose key challenge in its utilization. Cellulases from the glycoside hydrolase (GH) family 48 constitute an important component bacterial degrading systems and structures three enzymes this have been previously published. We report new crystal structure TfCel48A, reducing end directed exocellulase Thermobifida fusca, which shows that enzyme shares structural...
The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits proliferation and enhances differentiation human embryonal carcinoma cells. CREG binds to the cation-independent mannose 6-phosphate (M6P)/insulin-like growth factor II (IGF2) receptor (IGF2R) (M6P/IGF2R), this has been shown be required for CREG-induced suppression. To better understand function in differentiation, we solved 3D crystal structure protein 1.9-Å resolution. forms tight homodimeric...
A variety of catalytic and noncatalytic protein domains are deployed by select microorganisms to deconstruct lignocellulose. These extracellular proteins used attach to, modify, hydrolyze the complex polysaccharides present in plant cell walls. Cellulolytic enzymes, often containing carbohydrate-binding modules, key this process; however, these enzymes not solely responsible for attachment. Few mechanisms attachment have been discovered among bacteria that do form large polypeptide...
The green alga Chlamydomonas reinhardtii contains six plastidic [2Fe2S]-cluster ferredoxins (FDXs), with FDX1 as the predominant isoform under photoautotrophic growth. FDX2 is highly similar to and has been shown interact specific enzymes (such nitrite reductase), well share interactors FDX1, such hydrogenases (HYDA), ferredoxin:NAD(P) reductase I (FNR1), pyruvate:ferredoxin oxidoreductase (PFR1), albeit performing at low catalytic rates. Here we report crystal structure solved 1.18 Å...
The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF) was solved at 1.7 A resolution by the single wavelength anomalous dispersion (SAD) method. protein belongs to HutC subfamily large GntR transcriptional regulator family. Members this family share similar N-terminal DNA-binding domains, but are divided into four subfamilies according their heterogenic which involved in effector binding and oligomerization. C-PhnF provides for first time scaffold subfamily, covers about...