A5043058061- Microbial Community Ecology and Physiology
- bioluminescence and chemiluminescence research
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Methane Hydrates and Related Phenomena
- Gene Regulatory Network Analysis
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Protist diversity and phylogeny
- biodegradable polymer synthesis and properties
- Microbial Metabolic Engineering and Bioproduction
- Catalysis for Biomass Conversion
- Microfluidic and Bio-sensing Technologies
- Chromium effects and bioremediation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Advanced Cellulose Research Studies
- Plant biochemistry and biosynthesis
- Enzyme Production and Characterization
- Polyoxometalates: Synthesis and Applications
- thermodynamics and calorimetric analyses
National Renewable Energy Laboratory
2016-2025
CoorsTek (United States)
2024
Rice University
2020-2023
Northwestern University
2019
Abstract Ribo-T is a ribosome with covalently tethered subunits where core 16S and 23S ribosomal RNAs form single chimeric molecule. makes possible functionally orthogonal ribosome–mRNA system in cells. Unfortunately, use of has been limited because low activity its original version. Here, to overcome this limitation, we an evolutionary approach select new tether designs that are capable supporting faster cell growth increased protein expression. Further, evolve Ribo-T/mRNA pairs function...
Experimental evolution is a critical tool in many disciplines, including metabolic engineering and synthetic biology. However, current methods rely on the chance occurrence of key step that can dramatically accelerate natural systems, namely increased gene dosage. Our studies sought to induce targeted amplification chromosomal segments facilitate rapid evolution. Since dosage confers novel phenotypes genetic redundancy, we developed method, Evolution by Amplification Synthetic Biology...
Protein engineering and screening of processive fungal cellobiohydrolases (CBHs) remain challenging due to limited expression hosts, synergy-dependency, recalcitrant substrates. In particular, glycoside hydrolase family 7 (GH7) CBHs are critically important for the bioeconomy typically difficult engineer. Here, we target discovery highly active natural GH7 variants with improved activity. Using experimentally assayed activities genome mined CBHs, applied sequence structural alignments top...
Cupriavidus necator H16 is a facultative chemolithotroph capable of using CO2 as carbon source, making it promising organism for carbon-negative biomanufacturing petroleum-based product alternatives. In contrast to model microbes, genetic engineering technologies are limited in C. necator, constraining its utility basic and applied research. Here, we developed genome technology efficiently mobilize, integrate, express synthetic elements (SGEs) necator. We tested the chromosomal expression...
Pyrolysis offers a straightforward approach for the deconstruction of plant cell wall polymers into bio-oil. Recently, there has been substantial interest in bio-oil fractionation and subsequent use biological approaches to selectively upgrade some resulting fractions. A fraction particular upgrading consists polysaccharide-derived substrates including sugars sugar dehydration products such as levoglucosan cellobiosan, which are two most abundant pyrolysis cellulose. Levoglucosan can be...
Gene expression can be monitored in hard-to-image environmental materials using gas-reporting biosensors, but these outputs have only been applied autoclaved matrices that are hydrated with rich medium. To better understand the compatibility of indicator gas reporting samples, we evaluated how matrix hydration affects signal an engineered microbe added to a sieved soil. A presented forest soil (Alfisol) when environmentally relevant osmotic pressure. When was concentrated prior analysis,...
Monitoring microbial reactions in highly opaque or autofluorescent environments like soils, seawater, and wastewater remains challenging. To develop a simple approach for observing post-translational within microbes situated environmental matrices, we designed methyl halide transferase (MHT) fragment complementation assay that reports by synthesizing an indicator gas. We show backbone fission regions of high sequence variability the Rossmann domain yields split MHT (sMHT) AND gates whose...
Abstract It is challenging to detect microbial reactions in highly opaque or autofluorescent environments like soils, seawater, and wastewater. To develop a simple approach for monitoring post-translational within microbes situated environmental matrices, we designed methyl halide transferase (MHT) fragment complementation assay that reports by synthesizing an indicator gas. We show backbone fission regions of high sequence variability the Rossmann-fold domain yields split MHT (sMHT) AND...
ABSTRACT Gene expression can be monitored in hard-to-image environmental materials using gas-reporting biosensors, but these outputs have only been applied autoclaved matrices that are hydrated with rich medium. To better understand the compatibility of indicator gas reporting samples, we evaluated how matrix hydration affects signal an engineered microbe added to a sieved soil. A presented forest soil (Alfisol) when environmentally-relevant osmotic pressure. When was concentrated prior...
ABSTRACT Microbes can be found in abundance many kilometers underground. While microbial metabolic capabilities have been examined across different geochemical settings, it remains unclear how changes subsurface niches affect needs to sense and respond their environment. To address this question, we two component systems (TCS) vary metagenomes the Deep Mine Microbial Observatory (DeMMO). TCSs were at all six sites, service water control, surface site, with an average of 0.88 sensor histidine...
Microbes can be found in abundance many kilometers underground. While microbial metabolic capabilities have been examined across different geochemical settings, it remains unclear how changes subsurface niches affect needs to sense and respond their environment. To address this question, we extracellular sensor systems vary with environmental conditions metagenomes at Deep Mine Microbial Observatory (DeMMO) sites. Because two-component (TCSs) directly convert information into intracellular...