A5077488038- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Mycorrhizal Fungi and Plant Interactions
- Enzyme-mediated dye degradation
- Microbial Metabolites in Food Biotechnology
National Renewable Energy Laboratory
2024
CoorsTek (United States)
2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2019-2022
National Agricultural Technology Institute
2018-2022
Institute of Astronomy and Space Physics
2018
Instituto de Biodiversidad y Biología Experimental y Aplicada
2018
Experimental Medicine and Biology Institute
2018
University of Buenos Aires
2018
Protein engineering and screening of processive fungal cellobiohydrolases (CBHs) remain challenging due to limited expression hosts, synergy-dependency, recalcitrant substrates. In particular, glycoside hydrolase family 7 (GH7) CBHs are critically important for the bioeconomy typically difficult engineer. Here, we target discovery highly active natural GH7 variants with improved activity. Using experimentally assayed activities genome mined CBHs, applied sequence structural alignments top...
Abstract One of the main distinguishing features bacteria belonging to Cellulomonas genus is their ability secrete multiple polysaccharide degrading enzymes. However, application in biomass deconstruction still constitutes a challenge. We addressed optimisation xylanolytic activities extracellular enzymatic extracts sp. B6 and fimi B-402 for subsequent lignocellulosic hydrolysis by culture several substrates. As demonstrated secretomic profiling, wheat bran waste paper resulted be suitable...
In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for deconstruction β-glucans. this work, we focused on a GH8 endoglucanase (Cel8Pa) GH1 β-glucosidase (Bg1Pa) Paenibacillus xylanivorans A59. Cel8Pa was active broad range substrates, such as β-glucan barley (24.5 IU/mg), lichenan (17.9 phosphoric acid swollen cellulose (PASC) (9.7 carboxi-methylcellulose (CMC) (7.3 chitosan (1.4 IU/mg) xylan (0.4 IU/mg). Bg1Pa...