ABSTRACT Development of new drugs is one the strategies for malaria control. The biosynthesis several isoprenoids in Plasmodium falciparum was recently described. Interestingly, some intermediates and final products biosynthesized by this pathway mammals differ from those P. . These facts prompted us to evaluate various terpenes, molecules with a similar chemical structure pathway, as potential antimalarial drugs. Different terpenes S -farnesylthiosalicylic acid were tested on cultures...

Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is mitogen-activated protein kinase (MAPK) pathway that responds to variety stimuli, it central general stress response. Here we studied effect heat-stress (HS) HOG. Using live-cell reporters genetics, show HS promotes Hog1 phosphorylation Hog1-dependent gene expression, exclusively via Sln1 phosphorelay branch, strength activation larger in yeast adapted osmolarity....

Although the existence of O-linked oligosaccharide residues in glycoproteins Plasmodium falciparum has been shown, N-linked is still a matter controversy and skepticism.This report demonstrates unequivocal presence P. falciparum, principally ring young trophozoite stages intraerythrocytic cycle. These lose their capacity to bind concanavalin A-Sepharose after treatment cultures with tunicamycin under conditions that do not affect protein synthesis. When were treated N-Glycanase®,...

Crosstalk between MAPK pathways activated by a single sensory input enhances yeast responses to multiple simultaneous stimuli.

N-glycosylation of proteins is required for the intra-erythrocytic schizogony Plasmodium falciparum. In eukaryotic cells, this process involves transfer oligosaccharides from a dolichyl pyrophosphate derivative to asparagine residues. We have identified dolichol, phosphate and species 11 12 isoprenoid residues by metabolic labelling with [(3)H]farnesyl pyrophosphate, [(3)H]geranylgeranyl [(14)C]acetate in different stages P. This first demonstration short-chain dolichols phylum Apicomplexa....

ABSTRACT Isoprenylation is an essential protein modification in eukaryotic cells. Herein, we report that Plasmodium falciparum , a number of proteins were labeled upon incubation intraerythrocytic forms with either [ 3 H]farnesyl pyrophosphate or H]geranylgeranyl pyrophosphate. By thin-layer chromatography, showed attached isoprenoids are partially modified to dolichol and other, uncharacterized, residues, confirming active isoprenoid metabolism this parasite. Incubation blood-stage P....

Xanthomonas axonopodis pv. citri (Xac) causes citrus canker, provoking defoliation and premature fruit drop with concomitant economical damage. In plant pathogenic bacteria, lipopolysaccharides are important virulence factors, they being increasingly recognized as major pathogen-associated molecular patterns for plants. general, three domains in a lipopolysaccharide: the hydrophobic lipid A, hydrophilic O-antigen polysaccharide, core oligosaccharide, connecting A O-antigen. this work, we...

Cyclic beta-1,2-glucans (CbetaG) are osmolyte homopolysaccharides with a cyclic beta-1,2-backbone of 17-25 glucose residues present in the periplasmic space several bacteria. Initiation, elongation, and cyclization, three distinctive reactions required for building structure, catalyzed by same protein, CbetaG synthase. The initiation activity catalyzes transference first from UDP-glucose to yet-unidentified amino acid residue protein. Elongation proceeds successive addition nonreducing end...

Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from Bradyrhizobium japonicum are important for infection nodulation of soybean (Glycine max), although their roles not completely understood. To better understand this, we constructed mutants in B. USDA 110 impaired galactose or galacturonic acid incorporation into the EPS without affecting LPS. The derivative LP 3010 had a deletion lspL-ugdH produced whereas 3013, with an insertion exoB, galactose. In addition, strain 3017, both...

Trypanosoma cruzi , the parasitic protozoan that causes Chagas disease, contains a major cysteine proteinase, cruzipain. This lysosomal enzyme bears an unusual C‐terminal extension number of post‐translational modifications, and most antibodies in natural experimental infections are directed against it. In this report we took advantage UV‐MALDI‐TOF mass spectrometry conjunction with peptide N‐glycosidase F deglycosylation high performance anion exchange chromatography analysis to address...

The role of Mesorhizobium loti surface polysaccharides on the nodulation process is not yet fully understood. In this article, we describe phenotype mutants affected in synthesis lipopolysaccharide (LPS) and β(1,2) cyclic glucan. M. lps β2 mutant produces LPS with reduced amount O-antigen, whereas β1 totally devoid O-antigen. Both genes are clustered chromosome. Based amino acid sequence homology, sugar composition, enzymatic activity, concluded that codes for an enzyme involved...

Rhomboid proteases occur in all domains of life; however, their physiological role is not completely understood, and nothing known the biology these enzymes Archaea. One two rhomboid homologs Haloferax volcanii (RhoII) fused to a zinc finger domain. Chromosomal deletion rhoII was successful, indicating that this gene essential for organism; mutant strain (MIG1) showed reduced motility increased sensitivity novobiocin. Membrane preparations MIG1 were enriched glycoproteins, identified as...

The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [ 3 H]-palmitic acid and purified by immunoprecipitation a monodonal antibody. action PI-PLC on immunoprecipitate released lipid that analyzed TLC. Lyso-1- O -hexadecylglycerol N-palmitoyl-sphinganine were obtained in 1:3 ratio. A comparison GPI anchors present different stages T.cruzi made.

Abstract An increasing interest in the development of products natural origin for crop disease and pest control has emerged last decade. Here we introduce a new family strawberry acyl glycosides (SAGs) formed by trisaccharide (GalNAc-GalNAc-Glc) monounsaturated fatty acid 6 to 12 carbon atoms linked glucose unit. Application SAGs Arabidopsis thaliana (hereafter Arabidopsis) plants triggered transient oxidative burst, callose deposition defense gene expression, accompanied increased...

Malaria remains a major health problem especially in tropical and subtropical regions of the world, therefore developing new antimalarial drugs constitutes an urgent challenge. Lipid metabolism has been attracting lot attention as application for malarial chemotherapeutic purposes recent years. However, little is known about glycosphingolipid biosynthesis Plasmodium falciparum . In this report we describe first time presence active glucosylceramide synthase intraerythrocytic stages parasite....