A5082002650- Polysaccharides and Plant Cell Walls
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Biofuel production and bioconversion
- Multiple Myeloma Research and Treatments
- Microbial Metabolites in Food Biotechnology
- Mass Spectrometry Techniques and Applications
- Enzyme Production and Characterization
- Fluoride Effects and Removal
- Peptidase Inhibition and Analysis
- Cellular transport and secretion
- Bone and Dental Protein Studies
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Proteoglycans and glycosaminoglycans research
- Food composition and properties
- Analytical Chemistry and Chromatography
- Birth, Development, and Health
- Lipid Membrane Structure and Behavior
- Polysaccharides Composition and Applications
- Metabolomics and Mass Spectrometry Studies
- Protein Degradation and Inhibitors
- T-cell and B-cell Immunology
- CAR-T cell therapy research
University of California, San Francisco
2019-2024
Broad Center
2024
University of Georgia
2012-2017
Carbohydrate Competence Center
2010
Abstract Xyloglucan is a polysaccharide that has important roles in the formation and function of walls surround growing land plant cells. Many these plants synthesize xyloglucan contains galactose two different side chains (L F), which exist distinct molecular environments. However, little known about contribution to function. Here, we show Arabidopsis (Arabidopsis thaliana) mutants devoid F chain galactosyltransferase MURUS3 (MUR3) form lacks much less galactosylated xylose than its...
The myeloma surface proteome (surfaceome) determines tumor interaction with the microenvironment and serves as an emerging arena for therapeutic development. Here, we use glycoprotein capture proteomics to define surfaceome at baseline, in drug resistance, response acute treatment. We provide a scoring system antigens identify CCR10 promising target this disease expressed widely on malignant plasma cells. engineer proof-of-principle chimeric antigen receptor (CAR) T-cells targeting using its...
The mechanistic underpinnings of the complex process plant polysaccharide biosynthesis are poorly understood, largely because resistance glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes regiospecific transfer terminal 1,2-fucosyl residues xyloglucan side chains - key step in fucosylated sidechains galactoxyloglucan. We unravel basis for fucosylation by AtFUT1 with...
To facilitate our understanding of proteome dynamics during signaling events, robust workflows affording fast time resolution without confounding factors are essential. We present Surface-exposed protein Labeling using PeroxidaSe, H
Abstract Background Fluoride exposure during pregnancy has been associated with various effects on offspring, including changes in behavior and IQ. To provide clues to possible mechanisms by which fluoride may affect human fetal development, we completed proteomic analyses of cord blood serum collected from second-trimester pregnant women residing northern California, USA. Objective identify proteins maternal concentration women. Methods The proteomes 19 archived samples living USA, having...
ABSTRACT The myeloma cell surface proteome (“surfaceome”) not only determines tumor interaction with the microenvironment but serves as an emerging arena for therapeutic development. Here, we use glycoprotein capture proteomics to first define markers most-enriched on when compared B-cell malignancy models, revealing unexpected biological signatures unique malignant plasma cells. We next integrate our proteomic dataset existing transcriptome databases, nominating CCR10 and TXNDC11 possible...
Abstract To facilitate our understanding of the often rapid and nuanced dynamics extracellularly exposed proteomes during signaling events, it is important to devise robust workflows affording fast time resolution without biases confounding factors. Here, we present S urface-exposed protein La beling using P eroxida e, H 2 O , T yramide-derivative (SLAPSHOT), label proteins in a rapid, sensitive, specific manner, while preserving cellular integrity. This experimentally simple flexible method...
<title>Abstract</title> <bold>Background: </bold> Fluoride exposure during pregnancy has been associated with various effects on offspring, including changes in behavior and IQ. To provide clues to possible mechanisms by which fluoride affects human fetal development, we completed proteomic analyses of cord blood serum collected from second-trimester pregnant women residing Northern California either high or low exposure, as identified maternal concentrations. Objective: identify proteins...
The Multi-Omics for Mothers and Infants consortium aims to improve birth outcomes. Preterm is a major obstetrical complication globally causes significant infant childhood morbidity mortality.
Abstract Proteasome inhibitor (PI) resistance remains a central challenge in multiple myeloma. To identify pathways mediating resistance, we first map proteasome-associated genetic co-dependencies. We cytosolic heat shock protein 70 (HSP70) chaperones as potential targets, consistent with proposed mechanisms of myeloma tumor cells overcoming PI-induced stress. These results lead us to explore allosteric HSP70 inhibitors (JG compounds) therapeutics. show these compounds exhibit increased...
The plant cell wall is a complex extracytoplasmic organelle whose structure and dynamics are poorly understood at the molecular scale. Traditional biochemical methods have documented plasticity in properties during development response to stress, but limited characterizing bulk features. Alternatively, structural discriminating power of monoclonal antibodies can be utilized simultaneously probe abundances locations structures muro sub‐organellar resolution, examine components vitro . We...