A5027408781- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Hematopoietic Stem Cell Transplantation
- Polyomavirus and related diseases
- Eosinophilic Esophagitis
- Asthma and respiratory diseases
- Galectins and Cancer Biology
- Trauma and Emergency Care Studies
- Dermatology and Skin Diseases
- Xenotransplantation and immune response
- Allergic Rhinitis and Sensitization
- Innovations in Medical Education
- IL-33, ST2, and ILC Pathways
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
University of California, San Francisco
2020-2023
Center for Rheumatology
2023
The University of Queensland
2017-2020
Translational Research Institute
2017-2018
RELX Group (United States)
2017
University of the Philippines Diliman
2017
RELX Group (United Kingdom)
2017
University of Manitoba
2016
Teesside University
2015
B cell clones compete for entry into and dominance within germinal centers (GCs), where the highest-affinity receptors (BCRs) are selected. However, diverse low-affinity cells can enter reside in GCs extended periods. To reconcile these observations, we hypothesize that a negative feedback loop may operate to preferentially restrain high-affinity from monopolizing early GC niche. Here, report role nuclear receptor NUR77/Nr4a1 this process. We show NUR77 expression scales with antigen...
Human viruses possess very complex supramolecular structures. Both icosahedral and enveloped typically display an array of viral-encoded protein antigens at varied spatial densities on the viral particle surface. The nucleic acid genome, other hand, is encapsulated inside particle. Although both surface antigen interior acids could independently produce immunological responses, how B cells integrate these two types signals respond to a typical virus initiate activation not well understood...
Detecting naïve antigen-specific B cells can be challenging. Use of multiple, complementary tetramers with different fluorochromes enhances sensitivity and specificity allowing to readily distinguished within a polyclonal repertoire. Activated, affinity-matured cells, however, detected effectively using single tetramer.
Autoimmunity is characterized by loss of tolerance to tissue-specific as well systemic antigens, resulting in complex autoantibody landscapes. Here, we introduce and extensively validate the performance characteristics a murine proteome-wide library for phage display immunoprecipitation sequencing (PhIP-seq) profiling mouse autoantibodies. This was validated using 7 genetically distinct lines across spectrum autoreactivity. Mice deficient antibody production (Rag2-/- μMT) were used model...
Memory Th2 cell responses underlie the development and perpetuation of allergic diseases. Because these states result from immune dysregulation, established represent a significant challenge for conventional immunotherapies. New approaches that overcome detrimental effects dysregulation are required. We tested whether memory were silenced using therapeutic approach where allergen expression in DCs is transferred to sensitized recipients BM cells as vector gene transfer. Development...
Application of genetically modified hematopoietic stem cells is increasingly mooted as a clinically relevant approach to protein replacement therapy, immune tolerance induction or conditions where both outcomes may be helpful. Hematopoietic and progenitor cell (HSPC)-mediated gene therapy often requires highly toxic pretransfer recipient conditioning provide 'niche' so that transferred HSPCs can engraft effectively prevent rejection neoantigen-expressing engineered HSPCs. For widespread...
A series of layered peripheral checkpoints maintain self-reactive B cells in an unresponsive state. Autoantibody production occurs when these are breached; however, and how this is largely unknown. In particular, restrained during bystander inflammation otherwise healthy individuals poorly understood. weakness has been the unavailability methods capable dissecting physiologically relevant cell responses without use engineered BCR. Resolving will provide insights that decipher process goes...
Autoimmunity is characterized by loss of tolerance to tissue-specific as well systemic antigens, resulting in complex autoantibody landscapes. Here, we introduce and extensively validate the performance characteristics a murine proteome-wide library for phage display immunoprecipitation sequencing (PhIP-seq), profile mouse autoantibodies. This system were validated using seven genetic models across spectrum autoreactivity. Mice deficient antibody production (
Abstract Although it has long been appreciated that multivalent antigens – and particularly viral epitope display produce extremely rapid, robust, T-independent humoral immune responses, the biochemical basis for such potency incompletely understood. Here we take advantage of a set neutral liposomes size are engineered to affinity mutants model antigen (Ag) hen egg lysozyme at precisely varied density. We show particulate Ag by induces highly potent B cell responses dose-and...
ABSTRACT Ag stimulation (signal 1) triggers B cell activation and proliferation, primes cells to recruit, engage, respond T help 2). However, failure receive signal 2 within a defined window of time results in an abortive round followed by anergy or apoptosis. Although the molecular basis has been extensively dissected, mechanisms that restrain Ag-stimulated cells, enforce dependence upon co-stimulation, are incompletely understood. Nr4a1-3 encode small family orphan nuclear receptors...