A5051265755- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Eosinophilic Esophagitis
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Pain Mechanisms and Treatments
- Congenital Diaphragmatic Hernia Studies
- Escherichia coli research studies
- Early Childhood Education and Development
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Lymphatic System and Diseases
- Pharmacological Effects of Natural Compounds
- Cytokine Signaling Pathways and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Barrier Structure and Function Studies
- Respiratory Support and Mechanisms
Lund University
2013-2025
University of California, San Francisco
2018-2024
Universidad Católica de Santa Fe
2019
Peripheral nerve injury-induced neuropathic pain is a chronic and debilitating condition characterized by mechanical hypersensitivity. We previously identified microglial activation via release of colony-stimulating factor 1 (CSF1) from injured sensory neurons as mechanism contributing to pain. Here, we show that intrathecal administration CSF1, even in the absence injury, sufficient induce behavior, but only male mice. Transcriptional profiling morphologic analyses after CSF1 showed robust...
The innate immune system shapes brain development and is implicated in neurodevelopmental diseases. It critical to define the relevant cells signals their impact on circuits. In this work, we found that group 2 lymphoid (ILC2s) cytokine interleukin-13 (IL-13) signaled directly inhibitory interneurons increase synapse density developing mouse brain. ILC2s expanded produced IL-13 meninges. Loss of or signaling decreased inhibitory, but not excitatory, cortical synapses. Conversely, were...
The innate immune system relies to a great deal on the interaction of pattern recognition receptors with pathogen- or damage-associated molecular molecules. Extracellular histones belong latter group and their release has been described contribute induction systemic inflammatory reactions. However, little is known about functions in early response an invading pathogen. Here we show that extracellular specifically target monocytes human blood this evokes mobilization chemotactic chemokines...
Abstract Background Despite progress in the treatment of asthma, there is an unmet need for additional therapeutic strategies, not least to avoid side-effects corticosteroids. The enzyme MutT homolog 1 (MTH1) hydrolyzes oxidized purines and prevents their insertion DNA. Small molecule inhibition MTH1 has shown promising effects both cancer inflammatory conditions. In this study, a small inhibitor (TH1579), was investigated models allergic inflammation. Methods vitro, on T cell proliferation...
Type I interferons (IFNs) are essential for antiviral immunity, appear to represent a key component of mRNA vaccine-adjuvanticity, and correlate with severity systemic autoimmune disease. Relevant all, type IFNs can enhance germinal center (GC) B cell responses but underlying signaling pathways incompletely understood. Here, we demonstrate that succinct IFN response promotes GC formation associated IgG subclass distribution primarily through in cDCs cells. cDCs, distinct from cDC1,...
Antibody responses induced at mucosal and nonmucosal sites demonstrate a significant level of autonomy. Here, we key role for interferon regulatory factor-4 (IRF4)-dependent CD103
Systemic immunization with soluble flagellin (sFliC) from Salmonella Typhimurium induces mucosal responses, offering potential as an adjuvant platform for vaccines. Moreover, this engagement of immunity is necessary optimal systemic immunity, demonstrating interaction between these two semi-autonomous immune systems. Although TLR5 and CD103+CD11b+ cDC2 contribute to process, the relationship unclear in early activation T cells development antigen-specific B cell responses. In work, we use...
Cholera toxin (CT) binds to GM1-ganglioside receptors present on all nucleated cells. Despite this, it is a very potent mucosal adjuvant that has dramatic impact immune cells, as well nerve and epithelial causing diarrhea. This fact hampered our understanding of whether the adjuvanticity CT direct or indirect, cells bind may not be involved in its function. The barrier maintained by tight junctions between but dendritic (DCs) can protrude luminal dendrites. Here we investigated which are...
Abstract Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic (cDCs) are critical APCs for initial priming naive CD4+ but, importantly, also provide accessory signals govern cell commitment. To define the role cDCs during concurrent development Tfh and Th1 we performed high-dose Ag immunization in conjunction with Th1-biased adjuvant...
Fibroblasts coordinate the response to tissue injury, directing organ regeneration versus scarring. In central nervous system (CNS), fibroblasts are uncommon cells enriched at borders, and their molecular, cellular, functional interactions after brain injury poorly understood. Here we define fibroblast sterile damage across time space. Early pro-fibrotic myofibroblasts infiltrated CNS lesions were functionally spatially organized by TGF β signaling, macrophages microglia, perilesional glia...
Abstract The innate immune system plays essential roles in brain synaptic development, and dysregulation is implicated neurodevelopmental diseases. Here we show that a subset of lymphocytes (group 2 lymphoid cells, ILC2s) required for cortical inhibitory synapse maturation adult social behavior. ILC2s expanded the developing meninges produced surge their canonical cytokine Interleukin-13 (IL-13) between postnatal days 5-15. Loss decreased numbers period where as ILC2 transplant was...
SUMMARY Group 2 innate lymphoid cells (ILC2s) cooperate with adaptive Th2 as key organizers of tissue type immune responses, while a spectrum and lymphocytes coordinate early 3/17 immunity. Both lymphocyte associated cytokines are linked to fibrosis, but how their dynamic spatial topographies may direct beneficial or pathologic organ remodelling is unclear. Here we used volumetric imaging in models liver finding accumulation periportal fibrotic tract IL-5 + lymphocytes, predominantly ILC2s,...
Abstract Mobilization of type 2 immune responses is critical to both physiologic tissue remodeling and allergic pathology such as asthma. However, whether there are physical niches with unique cellular constituents that mediate immunity unknown. We used quantitative 3D-imaging define group innate lymphoid cells (ILC2), instigators immunity. identified a dominant adventitial ‘cuff’ niche around vessels, airways, ducts, present in the lung multiple other tissues. ILC2s, well subsets...
Abstract Type I interferons (IFNs) play an essential role in antiviral immunity, correlate with severity of systemic autoimmune disease, and are likely to represent a key component mRNA vaccine-adjuvanticity. Relevant all, type IFNs can enhance germinal center (GC) B cell responses but underlying signaling pathways incompletely understood due pleiotropic effects multiple types. Here, we demonstrate that succinct IFN response promotes GC formation associated IgG subclass distribution...