A5026503653- Neonatal and fetal brain pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Erythropoietin and Anemia Treatment
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Nuclear Receptors and Signaling
- Mitochondrial Function and Pathology
- Acute Ischemic Stroke Management
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Cancer, Hypoxia, and Metabolism
- Fetal and Pediatric Neurological Disorders
- Barrier Structure and Function Studies
- Immune Cell Function and Interaction
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Adenosine and Purinergic Signaling
University of California, San Francisco
2013-2024
As the resident macrophages of brain and spinal cord, microglia are crucial for phagocytosis infectious agents, apoptotic cells synapses. During injury or infection, bone-marrow derived invade neural tissue, making it difficult to distinguish between invading microglia. In addition circulation-derived monocytes, other non-microglial central nervous system (CNS) macrophage subtypes include border-associated meningeal, perivascular choroid plexus macrophages. Using immunofluorescent labeling,...
Background and Purpose— Stroke is a common cause of neonatal brain injury. The subventricular zone lifelong source newly generated cells in rodents, erythropoietin (EPO) treatment has shown benefit different animal models purpose this study to investigate the specific role exogenous EPO on progenitor cell populations response stroke. Methods— Intraventricular injections green fluorescent protein (GFP)–expressing lentivirus label precursor were made postnatal day 1 (P1) Long-Evans rats, which...
Perinatal stroke is a common cause of life-long neurobehavioral compromise. Mesenchymal stromal cells (MSCs) and EPO (erythropoietin) have each demonstrated short-term benefit with delayed administration after stroke, combination therapy may provide the most benefit. The purpose this study to determine long-term histological functional efficacy enhanced, intranasal stem cell (MSC preexposed EPO) compared standard MSC or multidose systemic EPO.
Abstract Background Neonatal stroke affects 1 in 2800 live births and is a major cause of neurological injury. The Sonic hedgehog (Shh) signaling pathway critical for central nervous system (CNS) development has neuroprotective reparative effects different CNS injury models. Previous studies have demonstrated beneficial small molecule Shh-Smoothened agonist (SAG) against neonatal cerebellar it improves Down syndrome-related brain structural deficits mice. Here we investigated SAG...
A number of animal models have been used to study hypoxic-ischemic injury, traumatic global hypoxia, or permanent ischemia in both the immature and mature brain. Stroke occurs commonly perinatal period humans, transient ischemia-reperfusion is most common form stroke neonates. The reperfusion phase a critical component injury progression, which over days weeks, endogenous response injury. This postnatal day 10 (p10) rat model middle cerebral artery occlusion (tMCAO) creates unilateral,...
Fibroblasts coordinate the response to tissue injury, directing organ regeneration versus scarring. In central nervous system (CNS), fibroblasts are uncommon cells enriched at borders, and their molecular, cellular, functional interactions after brain injury poorly understood. Here we define fibroblast sterile damage across time space. Early pro-fibrotic myofibroblasts infiltrated CNS lesions were functionally spatially organized by TGF β signaling, macrophages microglia, perilesional glia...
A number of animal models have been used to study hypoxic-ischemic injury, traumatic global hypoxia, or permanent ischemia in both the immature and mature brain. Stroke occurs commonly perinatal period humans, transient ischemia-reperfusion is most common form stroke neonates. The reperfusion phase a critical component injury progression, which over days weeks, endogenous response injury. This postnatal day 10 (p10) rat model middle cerebral artery occlusion (tMCAO) creates unilateral,...
Stroke is a major cause of death and disability in the United States. Current acute stroke therapy consists clot-dissolving drugs, catheter-based interventions physical rehabilitation. To date, there are no therapies that directly enhance neuronal survival after stroke. Previous work from our lab demonstrated Interleukin-15 (IL-15) peptide could rescue cardiomyocytes subjected to hypoxia. We sought extend these findings cortical neurons since IL-15 has been implicated have an important role...
Norepinephrine (NE) is a critical neuromodulator that mediates wide range of behavior and neurophysiology, including attention, arousal, plasticity, memory consolidation. A major source NE the brainstem nucleus locus coeruleus (LC), which sends widespread projections throughout central nervous system (CNS). Efforts to dissect this complex noradrenergic circuitry have driven development many tools detect endogenous or modulate release via LC activation inhibition. While these enabled research...
ABSTRACT Objective Neonatal stroke affects 1 in 2800 live births and is a major cause of neurological injury. The Sonic Hedgehog (Shh) signaling pathway critical for central nervous system (CNS) development has neuroprotective reparative effects different CNS injury models. Previous studies have demonstrated beneficial small molecule Shh-Smoothened-agonist (SAG) against neonatal cerebellar it improves Down syndrome-related brain structural deficits mice. Here, we investigated SAG...