A5073150379- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Communication and Nanonetworks
- Nerve injury and regeneration
- Immune cells in cancer
- Advanced Fluorescence Microscopy Techniques
- EEG and Brain-Computer Interfaces
- IoT and Edge/Fog Computing
- RNA Interference and Gene Delivery
- Gastrointestinal motility and disorders
- IL-33, ST2, and ILC Pathways
- Dermatological diseases and infestations
- Smart Agriculture and AI
- Ion Channels and Receptors
- Intestinal Malrotation and Obstruction Disorders
- Neonatal and fetal brain pathology
- Barrier Structure and Function Studies
- Eosinophilic Disorders and Syndromes
- Medicine and Dermatology Studies History
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
Neurological Surgery
2022-2024
University of California, San Francisco
2019-2024
Broad Center
2020-2024
Pennsylvania State University
2019-2020
Spinal cord injury (SCI) often leads to impaired motor and sensory functions, partially because the injury-induced neuronal loss cannot be easily replenished through endogenous mechanisms. In vivo reprogramming has emerged as a novel technology regenerate neurons from glial cells by forced expression of neurogenic transcription factors. We have previously demonstrated successful astrocyte-to-neuron conversion in mouse brains with or Alzheimer's disease overexpressing single neural factor...
We report quantitative label-free imaging with phase and polarization (QLIPP) for simultaneous measurement of density, anisotropy, orientation structures in unlabeled live cells tissue slices. combine QLIPP deep neural networks to predict fluorescence images diverse cell structures. reveal anatomical regions axon tract prenatal human brain sections that are not visible using brightfield imaging. a variant U-Net architecture, multi-channel 2.5D U-Net, computationally efficient prediction...
Progenitors of the developing human neocortex reside in ventricular and outer subventricular zones (VZ OSVZ, respectively). However, whether cells derived from these niches have similar developmental fates is unknown. By performing fate mapping primary tissue, we demonstrate that astrocytes populate anatomically distinct layers. Cortical plate emerge VZ progenitors proliferate locally, while putative white matter are morphologically heterogeneous both OSVZ progenitors. Furthermore, via...
The innate immune system shapes brain development and is implicated in neurodevelopmental diseases. It critical to define the relevant cells signals their impact on circuits. In this work, we found that group 2 lymphoid (ILC2s) cytokine interleukin-13 (IL-13) signaled directly inhibitory interneurons increase synapse density developing mouse brain. ILC2s expanded produced IL-13 meninges. Loss of or signaling decreased inhibitory, but not excitatory, cortical synapses. Conversely, were...
The Internet of Things (IoT) provides a simple framework to control online devices easily. IoT is now commonplace tool used by technology companies but rarely in biology experiments. can benefit cloud research through alarm notifications, automation, and the real-time monitoring We developed an architecture biological implemented it lab Lab for electrophysiology, microscopy, microfluidics were created from ground up be part unified architecture. system allows each device monitored controlled...
Abstract Microglia are the resident macrophages of brain that emerge in early development and play vital role disease states, as well normal development. Many fundamental questions about microglia diversity function during human remain unanswered, we currently lack cellular-resolution datasets focusing on developing primary tissue, or experimental strategies for interrogating their function. Here, report an integrative analysis throughout development, which reveals molecular signatures...
Quantitative imaging of biological architecture with fluorescent labels is not as scalable genomic or proteomic measurements. Here, we combine quantitative label-free and deep neural networks for analysis complex structures. We reconstruct three-dimensional density, anisotropy, orientation in live cells tissue slices from polarization- depth-resolved images. report a computationally efficient variant U-Net that predicts 3D structure its morphology physical properties. evaluate the...
Abstract The enteric nervous system (ENS) plays a central role in gut physiology and mediating the crosstalk between gastrointestinal (GI) tract other organs. human ENS has remained elusive, highlighting need for an vitro modeling mapping blueprint. Here we map out developmental functional features of ENS, by establishing robust scalable 2D cultures 3D ganglioids from pluripotent stem cells (hPSCs). These models recapitulate remarkable neuronal glial diversity found primary tissue enable...
Neural activity represents a functional readout of neurons that is increasingly important to monitor in wide range experiments. Extracellular recordings have emerged as powerful technique for measuring neural because these methods do not lead the destruction or degradation cells being measured. Current approaches electrophysiology low throughput experiments due manual supervision and expensive equipment. This bottleneck limits broader inferences can be achieved with numerous long-term...
Abstract GI toxicity is a common dose-limiting adverse effect of platin chemotherapy treatment. Up to 50% cancer survivors continue experience symptoms chronic constipation or diarrhea induced by their for many years after This drug largely attributed damage enteric neurons that innervate the tract and control motility. The mechanisms responsible platin-induced neurotoxicity potential preventative strategies have remained unknown. Here, we use human pluripotent stem cell derived establish...
Abstract Spinal cord injury (SCI) often leads to impaired motor and sensory functions, partially because the injury-induced neuronal loss cannot be easily replenished through endogenous mechanisms. In vivo reprogramming has emerged as a novel technology regenerate neurons from glial cells by forced expression of neurogenic transcription factors. We have previously demonstrated successful astrocyte-to-neuron conversion in mouse brains with or Alzheimer’s disease overexpressing single neural...
Abstract The Internet of Things (IoT) provides a simple framework to easily control online devices. IoT is now commonplace tool used by technology companies, but it rarely in biology experiments. can benefit research through alarm notifications, automation, and the real-time monitoring We developed implemented an architecture biological devices our own electrophysiology, microscopy, microfluidic so that may be controlled unified architecture. system allows each device monitored web tool....
New cell culture techniques have led to complex tissue models in biological experiments. For example, 3-D cerebral organoids provide a more realistic model of the human cortical tissue. However, these experiments are restricted by high costs and limited labor. A massively scalable cost efficient platform for would benefit genomics, neuroscience, translational medicine enabling advanced throughput screens. Cloud computing Internet Things (IoT) new tools managing multiple parallel that...
Abstract Objective Neural activity represents a functional readout of neurons that is increasingly important to monitor in wide range experiments. Extracellular recordings have emerged as powerful technique for measuring neural because these methods do not lead the destruction or degradation cells being measured. Current approaches electrophysiology low throughput experiments due manual supervision and expensive equipment. This bottleneck limits broader inferences can be achieved with...
Elisabeth Higgins, Anthony du Vivier Blackwell Science, £39.50, pp 277 ISBN 0 86542 835 2 Skin disease accounts for roughly 10-15% of all consultations in general practice, yet only a minute proportion the undergraduate curriculum is devoted to dermatology. Indeed, some students receive no dermatology training at and later on, as practitioners, may appreciable postgraduate subject. One hears too often practitioners their patients thumbing through textbooks together trying spot something...