A5065531893- Urinary Tract Infections Management
- Antimicrobial Resistance in Staphylococcus
- Nuclear Receptors and Signaling
- Macrophage Migration Inhibitory Factor
- Escherichia coli research studies
- Streptococcal Infections and Treatments
- Cytokine Signaling Pathways and Interactions
- Urinary Bladder and Prostate Research
- Pediatric Urology and Nephrology Studies
- Gut microbiota and health
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Biochemical and Structural Characterization
- Amino Acid Enzymes and Metabolism
- T-cell and B-cell Immunology
- Bladder and Urothelial Cancer Treatments
- Polyamine Metabolism and Applications
- Infective Endocarditis Diagnosis and Management
- Biopolymer Synthesis and Applications
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Diphtheria, Corynebacterium, and Tetanus
University of California, San Francisco
2021-2025
Washington University in St. Louis
2008-2013
ABSTRACT Though the bacterial opportunist Enterococcus faecalis causes a myriad of hospital-acquired infections (HAIs), including catheter-associated urinary tract (CAUTIs), little is known about virulence mechanisms that it employs. However, endocarditis- and biofilm-associated pilus (Ebp), member sortase-assembled family, was shown to play role in mouse model E. ascending UTI. The Ebp comprises major EbpC shaft subunit EbpA EbpB minor subunits. We investigated biogenesis function pili an...
Uropathogenic Escherichia coli (UPEC), the causative agent of approximately 85% urinary tract infections (UTI), is a major health concern primarily affecting women. During infection, neutrophils infiltrate bladder, but mechanism recruitment not well understood. Here, we investigated role UPEC-induced cytokine production in neutrophil and UTI progression. We first examined kinetics expression during UPEC infection their contribution to recruitment. found that induces several pro-inflammatory...
Enterococci commonly cause hospital-acquired infections, such as infective endocarditis and catheter-associated urinary tract infections. In animal models of these a long hairlike extracellular protein fiber known the endocarditis- biofilm-associated (Ebp) pilus is an important virulence factor for Enterococcus faecalis. For Ebp other sortase-assembled pili, pilus-associated sortases are essential formation they create covalent isopeptide bonds between sortase recognition motif pilin-like...
The Nr4a nuclear hormone receptors are transcriptionally upregulated in response to antigen recognition by the T cell receptor (TCR) thymus and implicated clonal deletion, but mechanisms which they operate not clear. Moreover, their role central tolerance is obscured redundancy among family members reported functions Treg generation maintenance. Here we take advantage of competitive bone marrow chimeras OT-II/RIPmOVA model show that Nr4a1 Nr4a3 essential for upregulation Bcl2l11/BIM thymic...
The NR4A family of orphan nuclear receptors (Nr4a1-3) plays redundant roles to establish and maintain Treg identity; deletion multiple members in the thymus results deficiency a severe inflammatory disease. Consequently, it has been challenging unmask functions other immune cells. Here we use competitive bone marrow chimera strategy, coupled with conditional genetic tools, rescue homeostasis such functions. Unexpectedly, chimeras harboring Nr4a1-/- Nr4a3-/- (double-knockout, DKO) developed...
Staphylococcus saprophyticus, an obligate human pathogen, is the most common Gram-positive causative agent of urinary tract infection (UTI) in young, healthy women. Despite clinical importance S. little known about how it causes disease or host responds to infection. Here we established vivo model study both and bacterial factors contributing saprophyticus UTI. Using this model, show that preferentially infects C3H/HeN murine kidneys instead bladder, a trait observed for multiple isolates....
ABSTRACT While many virulence factors promoting Streptococcus pyogenes invasive disease have been described, specific streptococcal and host properties influencing asymptomatic mucosal carriage remain uncertain. To address the need for a refined model of prolonged S. colonization, we adapted preestrogenized murine vaginal colonization . In this model, derivatives strains HSC5, SF370, JRS4, NZ131, MEW123 established reproducible, mucosa over period typically 3 to 4 weeks' duration at...
ABSTRACT Staphylococcus saprophyticus is the only species of that typically uropathogenic and possesses a gene coding for d -serine-deaminase (DsdA). As -serine prevalent in urine toxic or bacteriostatic to many bacteria, it not surprising found genome uropathogens. It has been suggested ability respond metabolize important virulence. For Escherichia coli (UPEC), high intracellular concentration affects expression virulence factors. S. able grow presence concentrations; however, its...
Abstract Enterococcus faecalis is a gram‐positive bacterium that part of the indigenous microbiotica humans and animals as well an opportunistic pathogen. In this study, we have fractionated membrane proteome E. identified many its constituents by mass spectrometry. We present blue native‐/SDS‐PAGE reference maps contain 102 proteins. These proteins are important for cellular homeostasis, virulence, antibiotic intervention. Intriguingly, with no known function were also identified,...
Abstract Although deletion of self-reactive thymocytes and their diversion into regulatory T cell (Treg) lineage are critical for immune tolerance homeostasis, the molecular pathways that link antigen recognition to these fates incompletely understood. The Nr4a nuclear hormone receptors transcriptionally upregulated in response TCR signaling thymus implicated both diversion, but mechanisms by which they operate not clear. Redundancy among family members requirement Treg generation...
In the article by Ingersoll et al. (2008) an error appeared in figure for Fig. 3E. The Y-axis was incorrectly labelled. with correctly labelled axis is shown below.
ABSTRACT The NR4A family of orphan nuclear receptors ( Nr4a1-3 ) plays redundant roles upstream Foxp3 to establish and maintain Treg identity; deletion multiple members in the thymus results deficiency a severe inflammatory disease. Consequently, it has been challenging isolate functions this other immune cells. Here we take advantage competitive bone marrow chimera strategy, coupled with conditional genetic tools, rescue homeostasis unmask such functions. Unexpectedly, chimeras harboring...