A5054716103- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Probiotics and Fermented Foods
- Diagnosis and treatment of tuberculosis
- COVID-19 epidemiological studies
- Pediatric health and respiratory diseases
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 detection and testing
- Immune Response and Inflammation
- Child Nutrition and Water Access
- Neuropeptides and Animal Physiology
- Heavy Metal Exposure and Toxicity
- Birth, Development, and Health
- Arsenic contamination and mitigation
- Herbal Medicine Research Studies
- Immune responses and vaccinations
- Ginseng Biological Effects and Applications
- Vibrio bacteria research studies
- Microbial Metabolism and Applications
- Preterm Birth and Chorioamnionitis
International Centre for Diarrhoeal Disease Research
2014-2025
Karolinska Institutet
2003-2020
National Institute of Cardiovascular Diseases
2020
London School of Hygiene & Tropical Medicine
2007
University of Arkansas for Medical Sciences
2007
Karolinska University Hospital
2003-2005
Shigella is a major cause of morbidity, mortality, and growth retardation for children in developing countries. Emergence antibiotic resistance among Shigellae demands the development effective medicines. Previous studies found that endogenous antimicrobial peptide LL-37 down-regulated rectal epithelium patients during shigellosis butyrate up-regulates expression colonic epithelial cells vitro decreases severity inflammation experimental shigellosis. In this study, Shigella-infected...
BackgroundAccurate quantitation of immune markers is crucial for ensuring reliable assessment vaccine efficacy against infectious diseases. This study was designed to confirm standardised performance SARS-CoV-2 assays used evaluate COVID-19 candidates at the initial seven laboratories (in North America, Europe, and Asia) Coalition Epidemic Preparedness Innovations (CEPI) Centralized Laboratory Network (CLN).MethodsThree ELISAs (pre-spike protein, receptor binding domain, nucleocapsid), a...
Abstract COVID-19 vaccines have been effective in preventing severe illness, hospitalization and death, however, the effectiveness diminishes with time. Here, we evaluated longevity of antibodies generated by COIVD-19 risk (re)infection Bangladeshi population. Adults receiving two doses AstraZeneca, Pfizer, Moderna or Sinopharm were enrolled at 2–4 weeks after second dosing followed-up 4-monthly interval for 1 year. Data on COVID-like symptoms, confirmed infection, co-morbidities, receipt...
Background Cathelicidins and defensins are endogenous antimicrobial peptides (AMPs) that downregulated in the mucosal epithelia of large intestine shigellosis. Oral treatment Shigella infected rabbits with sodium butyrate (NaB) reduces clinical severity counteracts downregulation cathelicidin (CAP-18) intestinal epithelia. Aims To develop novel regimen for treating infectious diseases by inducing innate immunity, we selected 4-phenylbutyrate (PB), a registered drug metabolic disorder as...
Treatment of shigellosis in rabbits with butyrate reduces clinical severity and counteracts the downregulation cathelicidin (CAP-18) large intestinal epithelia. Here, we aimed to evaluate whether can be used as an adjunct antibiotics treatment patients. A randomized, double-blind, placebo-controlled, parallel-group designed trial was conducted. Eighty adult patients were randomized either Intervention group (butyrate, n = 40) or Placebo (normal saline, 40). The given enema containing sodium...
Abstract Background We earlier showed that 4-phenylbutyrate (PB) can induce cathelicidin LL-37 expression synergistically with 1,25-dihydroxyvitamin D 3 in a lung epithelial cell line. aimed to evaluate therapeutic dose of PB alone or combination vitamin for induction immune cells and enhancement antimycobacterial activity monocyte-derived macrophages (MDM). Methods Healthy volunteers were enrolled an 8-days open trial three doses [250 mg (Group-I), 500 (Group-II) 1000 (Group-III)] twice...
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Background: Vaccination has played a crucial role in mitigating the spread of COVID-19 and reducing its severe outcomes. While over 90% Bangladesh’s population received at least one vaccine dose, comparative effectiveness homologous versus heterologous booster strategies, along with complex interplay factors within population, remains understudied. This study aimed to compare antibody responses between these approaches. Methods: cross-sectional enrolled 723 adults urban Dhaka who had doses...
Abstract A new concept for treatment of infections is induction our own antimicrobial peptides and the presented novel class inducer, aroylated phenylenediamines (APDs), gives up to 20 30-fold human peptide LL-37, in vitro . In addition, oral administration an APD a rabbit model Shigellosis resulted recovery from infection few days implying that APD’s are promising candidates infections.
Shigella sonnei live vaccine candidate, WRSS1, which was previously evaluated in US, Israeli and Thai volunteers, administered orally to Bangladeshi adults children assess its safety, clinical tolerability immunogenicity. In a randomized, placebo-controlled, dose-escalation, age-descending study, 39 (18–39 years) 64 (5–9 were enrolled. Each adult cohort (n = 13) received one dose of 3x104, or three doses 3 × 105 106 colony forming unit (CFU) WRSS1 10) placebo 3). child 16) 3x103, 3x105, CFU...
The adaptive immune response is a crucial component of the protective immunity against SARS-CoV-2, generated after infection or vaccination.We studied antibody titers, neutralizing antibodies and cellular responses to four different COVID-19 vaccines, namely Pfizer-BioNTech, Moderna Spikevax, AstraZeneca Sinopharm vaccines in Bangladeshi population (n = 1780).mRNA (14,655 ± 11.3) Pfizer (13,772 11.5) elicited significantly higher anti-Spike (S) titers compared Adenovector vaccine (2443 12.8)...
We hypothesized that increased susceptibility to Shigella infection, severity of disease and high mortality in children compared with adults were consequences insufficient adaptive immune responses. Antigen‐specific responses studied paediatric patients ( n = 38, 2–10 years) shigellosis those adult 30, 18–45 years). Peak frequencies antigen (invasion plasmid coded B, Ipa‐B; lipopolysaccharide, LPS)‐specific immunoglobulin (IgM)‐antibody secreting cells (ASC) seen within 3–5 days after the...
Background Seroprevalence studies have been carried out in many developed and developing countries to evaluate ongoing past infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Data on this marginalized populations urban slums are limited, which may offer crucial information update prevention mitigation policies strategies. We aimed determine the seroprevalence of SARS-CoV-2 factors associated seropositivity slum non-slum communities two large cities Bangladesh....
ABSTRACT Cells of the innate immune system and their mediators were studied at single-cell level in rectums pediatric adult patients with Shigella infection to better understand why children are higher risk for severe infection. Adult had increased infiltration mucosal mast cells (MMC) acute stage (3 5 days after onset diarrhea) eosinophils early convalescence (14 16 onset). Increased expression stem cell factor prostaglandin H synthase-1 (PGHS-1) was associated tryptase- K i...
Antibiotics exert several effects on host cells including regulation of immune components. Antimicrobial peptides (AMPs), e.g., cathelicidins and defensins display multiple functions in innate immunity. In colonic mucosa, are induced by butyrate, a bacterial fermentation product. Here, we investigated the effect antibiotics butyrate-induced expression beta-defensins colon epithelial cells. Real-time PCR analysis revealed that ciprofloxacin clindamycin reduce transcription human cathelicidin...
ABSTRACT We have shown previously that oral treatment with sodium butyrate or phenylbutyrate in an experimental model of shigellosis improves clinical outcomes and induces the expression antimicrobial peptide CAP-18 large intestinal epithelia. In a subsequent study, we found entinostat, aroylated phenylenediamine compound, has similar therapeutic potential against shigellosis. this aimed to evaluate entinostat as candidate for host-directed therapy cholera model. Vibrio cholerae -infected...
The study of cellular immunity to SARS-CoV-2 is crucial for evaluating the course COVID-19 disease and improving vaccine development. We aimed assess phenotypic landscape circulating lymphocytes mononuclear cells in adults children who were seropositive past 6 months. Blood samples (n = 350) collected a cross-sectional Dhaka, Bangladesh (Oct 2020-Feb 2021). Plasma antibody responses determined by an electrochemiluminescence immunoassay while lymphocyte monocyte assessed using flow cytometry...
We demonstrated in a randomized placebo-controlled trial that WRSS1, live oral Shigella sonnei vaccine candidate, is safe Bangladeshi adults and children, elicits antigen-specific antibodies. Here, we describe functional antibody innate immune responses to WRSS1.Adults (18-39 years) children (5-9 received 3 doses of × 105 or 106 colony forming units (CFU) WRSS1 placebo, 4 weeks apart; additionally 104 CFU. Blood stool were collected at baseline 7 days after each dose. Functional antibodies...