A5051602267- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Organophosphorus compounds synthesis
- RNA Interference and Gene Delivery
- Synthesis and Characterization of Heterocyclic Compounds
- Chemical Synthesis and Analysis
- RNA modifications and cancer
- Click Chemistry and Applications
- Chemical Reaction Mechanisms
- Carbohydrate Chemistry and Synthesis
- HIV/AIDS drug development and treatment
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Alzheimer's disease research and treatments
- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- Pediatric health and respiratory diseases
- RNA Research and Splicing
- Polyamine Metabolism and Applications
- Adenosine and Purinergic Signaling
- Biochemical and Structural Characterization
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Immune Response and Inflammation
- Fluorine in Organic Chemistry
Karolinska Institutet
2015-2024
Integrated Cardio Metabolic Centre
2015-2017
University of Turku
2014
Södertörn University
2014
Centre for Family Medicine
2013
Karolinska University Hospital
2008-2011
Swedish University of Agricultural Sciences
2009
Riga Technical University
2003
Saha Institute of Nuclear Physics
2000
Stockholm University
1988-2000
Background: Knowing the levels of polyamines (putrescine, spermidine, and spermine) in different foods is interest due to association these bioactive nutrients health diseases. There a lack relevant information on their contents foods. Objective: To develop food polyamine database from published data by which intake contribution this can be estimated, determine Swedish dairy products. Design: Extensive literature search laboratory analysis selected Polyamine were collected using an extensive...
The amyloid-β peptide (Aβ) can generate cytotoxic oligomers, and their accumulation is thought to underlie the neuropathologic changes found in Alzheimer's disease. Known inhibitors of Aβ polymerization bind undefined structures work as nonspecific aggregators, that target conformations also occur larger assemblies may even increase oligomer-derived toxicity. Here we report on an alternative approach whereby ligands are designed stabilize 13–26 region α-helical conformation, inspired by...
Postpartum, infants have not yet established a fully functional adaptive immune system and are at risk of acquiring infections. Hence, newborns dependent on the innate with its antimicrobial peptides (AMPs) proteins expressed epithelial surfaces. Several factors in breast milk known to confer protection, but which decisive through manner they work is unknown. Here, we isolated an AMP-inducing factor from human identified it by electrospray mass spectrometry NMR be lactose. It induces gene...
The amyloid-β hypothesis of Alzheimer's Disease (AD) focuses on accumulation peptide (Aβ) as the main culprit for myriad physiological changes seen during development and progression AD including desynchronization neuronal action potentials, consequent aberrant brain rhythms relevant cognition, final emergence cognitive deficits. aim this study was to elucidate cellular synaptic mechanisms underlying Aβ-induced degradation gamma oscillations in AD, identify aggregation state(s) Aβ that...
G-quadruplex (G4) DNA structures are prevalent secondary implicated in fundamental cellular functions, such as replication and transcription. Furthermore, G4 directly correlated to human diseases cancer have been highlighted promising therapeutic targets for their ability regulate disease-causing genes, e.g., oncogenes. Small molecules that bind stabilize these thus valuable from a perspective helpful studying the biological functions of structures. However, there hundreds thousands motifs...
Background Cathelicidins and defensins are endogenous antimicrobial peptides (AMPs) that downregulated in the mucosal epithelia of large intestine shigellosis. Oral treatment Shigella infected rabbits with sodium butyrate (NaB) reduces clinical severity counteracts downregulation cathelicidin (CAP-18) intestinal epithelia. Aims To develop novel regimen for treating infectious diseases by inducing innate immunity, we selected 4-phenylbutyrate (PB), a registered drug metabolic disorder as...
The cha nges in chemical structure occu ring pol y (vinyl chloride) as a res ul t of heati ng ina vacu um he range 100° to 400° C wer e studied using infrared spectropho metry.The pri ncip l changes occ urri residue during p yrolysis vacuum were the format ion unsaturated s tructures nd change from n al iphatic spectrum one showing aroma tic absorption.The data are used suppor previou sly proposed mechani sm decomposition for poly .
RNA-based therapeutics are emerging as a powerful platform for the treatment of multiple diseases. Currently, two main categories nucleic acid therapeutics, antisense oligonucleotides and small interfering RNAs (siRNAs), achieve their therapeutic effect through either gene silencing, splicing modulation or microRNA binding, giving rise to versatile options target pathogenic expression patterns. Moreover, ongoing research seeks expand scope drugs include more complex templates, such messenger...
Journal Article Removal of f-butyldimethylsilyl protection in RNA-synthesis. Triethylamine trihydrofluoride (TEA, 3HF) is a more reliable alternative to tetrabutylammonium fluoride (TBAF) Get access Erik Westmanu, Westmanu Department Organic Chemistry, Arrhenius Laboratory, Stockholm UniversityS-10691 Stockholm,Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar Roger Stromberg * To whom correspondence should be addressed Nucleic Acids Research, Volume 22,...
Abstract Recombinant spider silk proteins (spidroins) have multiple potential applications in development of novel biomaterials, but their multimodal and aggregation-prone nature complicated production straightforward applications. Here, we report that recombinant miniature spidroins, importantly also the N-terminal domain (NT) on its own, rapidly form self-supporting transparent hydrogels at 37 °C. The gelation is caused by NT α-helix to β-sheet conversion formation amyloid-like fibrils,...
Native pulmonary-surfactant-associated lipopolypeptide SP-C, its chemically depalmitoylated form and several synthetic analogues lacking the palmitoylcysteine residues were analysed for secondary structure in phospholipid micelles biophysical activity 1,2-dipalmitoyl-sn-glycero-3- phosphocholine/phosphatidylglycerol/palmitic acid (68:22:9, by wt.). Compared with native molecule, entire poly-valyl part a known alpha-helical conformation, SP-C was found to be still mainly alpha-helical, but an...
Two model compounds, 1 and 2, have been studied to test the stability of t-butyldimethylsilyl (t-BDMSi) group towards conditions used during chemical synthesis RNA fragments by H-phosphonate approach. When was treated with anhydrous acid for 16 h both diester t-BDMSi remained intact. Removal from 2 1.0 M tetrabutylammonium fluoride (TBAF- 3H2O) in THF complete within 4 neither concomitant cleavage nor migration phospho-diester linkage could be detected even after 24 h. The dimer not...
DNA-cleaving restriction enzymes are well-known tools in biomedical and biotechnological research. There are, however, no corresponding known for RNA cleavage. has been an ongoing development of artificial ribonucleases, including some attempts at sequence selectivity. However, so far these systems have displayed modest rates cleavage, most cases, the cleaver used excess or stoichiometric amounts. In current work, we present PNA-based (PNAzymes) that carry a Cu(II)-2,9-dimethylphenanthroline...
Abstract Bacterial resistance against classical antibiotics is a growing problem and the development of new limited. Thus, novel alternatives to are warranted. Antimicrobial peptides (AMPs) effector molecules innate immunity that can be induced by several compounds, including vitamin D phenyl-butyrate (PBA). Utilizing luciferase based assay, we recently discovered histone deacetylase inhibitor Entinostat potent inducer CAMP gene encoding human cathelicidin LL-37. Here investigate mechanism...
Two different forms of the monomeric NADP(+)-linked prostaglandin dehydrogenase/carbonyl reductase were purified from human placenta and shown to differ by modification a lysine residue. The modified unmodified proteins reproducibly recovered in ratio approximately 1:3, both chemically stable. form was more acidic (pI 7.4 versus pI 7.7) but indistinguishable specificity activity. Amino acid analysis, sequence mass spectrometry, chemical synthesis identified residue as...
A Bronsted graph with a convex break at pK(a)(Lg)= 12.58 provides compelling evidence for an intermediate in the alkaline cyclisation of uridine 3'-phosphate esters. The transient pentacoordinated oxyphosphorane dianion collapses to reactant and cyclic 2',3'-monophosphate faster than it can pseudo-rotate isomerise 2'-isomer.
Oligoribonucleotide analogues having amide internucleoside linkages (AM1: 3'-CH2CONH-5' and AM2: 3'-CH2NHCO-5') at selected positions have been synthesized the thermal stability of duplexes formed by these with complementary RNA fragments has evaluated UV melting experiments. Two series oligomers either 2'-OH or 2'-OMe vicinal to were studied. Monomeric synthons (3' 5'-C amines carboxylic acids) as follows: For synthesis AM1 analogue, known sequence radical allylation followed cleavage...
Oxidation of phosphonate mono- and di-esters with various oxidizing agents including diaryl disulphides, hexachloroacetone, iodine, has been investigated under reaction conditions. The most efficient oxidation procedure consists treatment esters iodine in pyridine–water. When the were presilylated by trimethylsilyl chloride, subsequent aqueous was faster. 31P N.m.r. spectroscopic studies have enabled us to propose likely pathway for majority oxidations.
Polyamine intake from milk is considered essential for post-natal maturation of the immune system and small intestine. The present study aimed to determine polyamine content in human after preterm delivery association with mothers' dietary intake. In comparison, levels were compared those term breast some corresponding formulas.Transitional was collected 40 mothers delivering 24-36 weeks gestation, 12 full term. Food assessed babies using a 3-day diary. Polyamines analysed by...
Abstract A new concept for treatment of infections is induction our own antimicrobial peptides and the presented novel class inducer, aroylated phenylenediamines (APDs), gives up to 20 30-fold human peptide LL-37, in vitro . In addition, oral administration an APD a rabbit model Shigellosis resulted recovery from infection few days implying that APD’s are promising candidates infections.
Two novel bicyclo[6.1.0]nonyne (BCN) linker derivatives, which can be directly incorporated into oligonucleotide sequences during standard automated solid-phase synthesis, are reported. Stabilities of BCN-carbinol and two BCN-oligonucleotides evaluated under acidic conditions. In addition, derivatized BCN linkers (non-acidic acid treated) for strain-promoted alkyne-azide cycloaddition (SPAAC).
A series of uridine 3′-alkyl phosphates and 3′-aryl were synthesised their cleavage was studied in the presence Zn2+ aquo ions. βlg value determined for promoted both types compounds. Comparison results obtained to those reported previously same substrates absence metal ion catalysts suggests that alkyl leaving group departs as an alcohol catalysts. Furthermore, seem enhance departure. The aryl group, contrast, oxyanion.