A5031148121- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Microwave and Dielectric Measurement Techniques
- Epigenetics and DNA Methylation
- Renal and related cancers
- Biosensors and Analytical Detection
- Protein Interaction Studies and Fluorescence Analysis
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related Molecular Pathways
McGill University
2017-2021
Institute of Molecular and Cell Biology
2018-2019
Agency for Science, Technology and Research
2018-2019
Fluorescent dye labeling of DNA oligonucleotides and nanostructures is one the most used techniques to track their fate cellular localization inside cells. Here, we report that intracellular fluorescence, even FRET signals, cannot be correlated with uptake intact structures. Live cell imaging revealed high colocalization cyanine-labeled oligos phosphorylated small-molecule cyanine dyes, degradation products from these compounds. Nuclease strands outside results in a misleading fluorescent...
The development of nucleic acid therapeutics has been hampered by issues associated with their stability and in vivo delivery. To address these challenges, we describe a new strategy to engineer DNA structures strong binding affinity human serum albumin (HSA). HSA is the most abundant protein blood long circulation half-life (19 days). It shown hinder phagocytosis, retained tumors, aids cellular penetration. Indeed, already successfully used for delivery small-molecule drugs nanoparticles....
Cell cycle regulation, especially faithful DNA replication and mitosis, are crucial to maintain genome stability. Cyclin-dependent kinase (CDK)/cyclin complexes drive most processes in cellular proliferation. In response damage, cell surveillance mechanisms enable normal cells arrest undergo repair processes. Perturbations genomic stability can lead tumor development suggest that regulators could be effective targets anticancer therapy. However, many clinical trials ended failure due...
RNA-based therapeutics are emerging as a powerful platform for the treatment of multiple diseases. Currently, two main categories nucleic acid therapeutics, antisense oligonucleotides and small interfering RNAs (siRNAs), achieve their therapeutic effect through either gene silencing, splicing modulation or microRNA binding, giving rise to versatile options target pathogenic expression patterns. Moreover, ongoing research seeks expand scope drugs include more complex templates, such messenger...
RNA has inherent therapeutic and structural properties that make it an important component of biologically-functional nanoparticles. Using DNA-amphiphiles as synthetic templates, we report the synthesis two classes RNA-amphiphiles self-assemble into spherical nanoparticles in aqueous solution show gene silencing activity.