A5048448106- Silk-based biomaterials and applications
- Biochemical and Structural Characterization
- Adhesion, Friction, and Surface Interactions
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Chemical Synthesis and Analysis
- Silkworms and Sericulture Research
- Electrospun Nanofibers in Biomedical Applications
- Surface Modification and Superhydrophobicity
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Studies on Chitinases and Chitosanases
- Enzyme Production and Characterization
- Art Therapy and Mental Health
- Neurological diseases and metabolism
- Protease and Inhibitor Mechanisms
- Invertebrate Immune Response Mechanisms
- Peptidase Inhibition and Analysis
- Digital Innovation in Industries
- Antimicrobial Peptides and Activities
- Advanced Surface Polishing Techniques
- Phytochemical compounds biological activities
- RNA modifications and cancer
- Fashion and Cultural Textiles
- Web and Library Services
Karolinska Institutet
2020-2023
University of Cambridge
2014
Abstract Spider silk is the toughest fiber found in nature, and bulk production of artificial spider that matches its mechanical properties remains elusive. Development miniature proteins (mini‐spidroins) has made large‐scale economically feasible, but fibers’ are inferior to native silk. The fiber's tensile strength conferred by poly‐alanine stretches zipped together tight side chain packing β‐sheet crystals. Spidroins secreted so they must be void long hydrophobic residues, since such...
Many protein condensates can convert to fibrillar aggregates, but the underlying mechanisms are unclear. Liquid–liquid phase separation (LLPS) of spider silk proteins, spidroins, suggests a regulatory switch between both states. Here, we combine microscopy and native mass spectrometry investigate influence sequence, ions, domains on spidroin LLPS. We find that salting out-effects drive LLPS via low-affinity stickers in repeat domains. Interestingly, conditions enable simultaneously cause...
Abstract Water and humidity severely affect the material properties of spider major ampullate silk, causing fiber to become plasticized, contract, swell undergo torsion. Several amino acid residue types have been proposed be involved in this process, but complex composition native complicates detailed investigations. Here, we observe supercontraction biomimetically produced artificial silk fibers composed defined proteins. We found experimental evidence that proline is not sole responsible...
Liquid–liquid phase separation (LLPS) of heterogeneous ribonucleoproteins (hnRNPs) drives the formation membraneless organelles, but structural information about their assembled states is still lacking. Here, we address this challenge through a combination protein engineering, native ion mobility mass spectrometry, and molecular dynamics simulations. We used an LLPS-compatible spider silk domain pH changes to control self-assembly hnRNPs FUS, TDP-43, hCPEB3, which are implicated in...
Hydrogels are useful drug release systems and tissue engineering scaffolds. However, synthetic hydrogels often require harsh gelation conditions can contain toxic by-products while naturally derived transmit pathogens in general have poor mechanical properties. Thus, there is a need for hydrogel that forms under ambient conditions, non-toxic, xeno-free, has good A recombinant spider silk protein-derived rapidly at 37 °C recently developed. The temperature times well-suited an injectable situ...
Abstract Recombinant spider silk proteins (spidroins) have multiple potential applications in development of novel biomaterials, but their multimodal and aggregation-prone nature complicated production straightforward applications. Here, we report that recombinant miniature spidroins, importantly also the N-terminal domain (NT) on its own, rapidly form self-supporting transparent hydrogels at 37 °C. The gelation is caused by NT α-helix to β-sheet conversion formation amyloid-like fibrils,...
Efficient production of artificial spider silk fibers with properties that match its natural counterpart has still not been achieved. Recently, a biomimetic process for spinning recombinant proteins (spidroins) was presented, in which important molecular mechanisms involved native were recapitulated. However, drawbacks these included inferior mechanical and problems low resistance to aqueous environments. In this work, we show ≥5 h incubation the fibers, collection bath 500 mM NaAc 200 NaCl,...
Recombinant spider silk has emerged as a biomaterial that can circumvent problems associated with synthetic and naturally derived polymers, while still fulfilling the potential of native material. The artificial protein NT2RepCT be produced spun into fibers without use harsh chemicals here we evaluate key properties dope at native-like concentrations. We show recapitulates not only overall secondary structure content but also emulates its viscoelastic rheological properties. propose these...
ABSTRACT Prion diseases are characterized by a conformational change in the normal host protein PrPC. While majority of mature PrPC is tethered to plasma membrane glycosylphosphatidylinositol anchor, topological variants this can arise during its biosynthesis. Here we have generated Drosophila transgenic for cytosolic ovine PrP order investigate toxic potential flies absence or presence exogenous prions. expressed was predominantly detergent insoluble and showed resistance low concentrations...
Silk fibers have unique mechanical properties, and many studies of silk aim at understanding how these properties are related to secondary structure content, which often is determined by infrared spectroscopy. We report significant method-induced irreversible structural changes both natural synthetic spider fibers, derived from the widely used attenuated total reflection Fourier-transform (ATR-FTIR) technique. By varying force bring into contact with internal elements ATR-FTIR accessories,...
Surgical intervention with the use of autografts is considered gold standard to treat peripheral nerve injuries. However, a biomaterial that supports and guides growth would be an attractive alternative overcome problems limited availability, morbidity at site harvest, mismatches related autografts. Native spider silk promising material for construction guidance conduit (NGC), as it enables regeneration cm-long injuries in sheep, but regulatory requirements medical devices demand synthetic...
Engineered Spider Silk Protein In article number 2200986, Anna Rising and co-workers present the impressive strength of spider silk fibers conferred by poly-alanines in stacked β-sheets (steric zippers pink). By protein engineering expression prokaryotic hosts, they have done what spiders cannot do: increase hydrophobicity intermolecular interactions steric (turquoise) thereby generating stronger artificial silk.
Abstract Phase separation of heterogeneous ribonucleoproteins (hRNPs) drives the formation membraneless organelles, but structural information about their assembled states is still lacking. Here, we address this challenge through a combination protein engineering, native ion mobility-mass spectrometry, and molecular dynamics simulations. We used phase separation-compatible spider silk domain pH changes to control self-assembly hRNPs FUS, TDP-43, hCPEB3, which are implicated in...