A5102871014- Viral gastroenteritis research and epidemiology
- Health and Medical Education
- SARS-CoV-2 and COVID-19 Research
- Vaccine Coverage and Hesitancy
- Salmonella and Campylobacter epidemiology
- Ethics and bioethics in healthcare
- Child Nutrition and Water Access
- Escherichia coli research studies
- Helicobacter pylori-related gastroenterology studies
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Global Maternal and Child Health
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Animal Virus Infections Studies
- Medicine and Dermatology Studies History
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Hepatitis Viruses Studies and Epidemiology
- Healthcare Systems and Reforms
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Viral Infections and Vectors
Universidad Nacional Autónoma de México
2008-2025
International Centre for Diarrhoeal Disease Research
1970-2019
Universidad Marista
2015
International Vaccine Institute
2013-2014
GlaxoSmithKline (Belgium)
2013
Bangabandhu Sheikh Mujib Medical University
2009
Karolinska University Hospital
2009
Karolinska Institutet
2009
World Health Organization - Zimbabwe
2009
Instituto Nacional de Enfermedades Respiratorias
2003
Multilocus enzyme electrophoresis was employed to measure chromosomal genotypic diversity and evolutionary relationships among 761 isolates of the serovars Salmonella typhi, S. paratyphi A, B, C, sendai, which are human-adapted agents enteric fever, miami java, serotypically similar sendai respectively, but cause gastroenteritis in both humans animals. To determine phylogenetic positions clones these forms within context salmonellae subspecies I, comparative data for 22 other common were...
Variation in the chromosomal genomes of 1527 isolates eight common serotypes (O and H antigen profiles) Salmonella was assessed by analysis electrophoretically demonstrable allelic polymorphism at 23 metabolic enzyme loci. Seventy-one distinctive electrophoretic types, representing multilocus genotypes, were identified. A basically clonal population structure indicated presence strong linkage disequilibrium among loci, association each serotype with a relatively small number global...
SUMMARY Colonization of the intestine by putative pathogens was followed longitudinally in a cohort 56 infants born during one calendar year rural Mexican village with faecal cultures taken every fortnight and time child had diarrhoea. The frequency isolation episodes diarrhoea compared that matched controls from same cohort. Incidence first life 98%, diminishing to 93% second year. incidence curves for each were not significantly different ( P > 0·1). Isolation enteropathogenic...
Commonly used trivalent vaccines contain one influenza B virus lineage and may be ineffective against viruses of the other lineage. We evaluated efficacy a candidate inactivated quadrivalent vaccine (QIV) containing both lineages.In this multinational, phase 3, observer-blinded study, we randomly assigned children 3 to 8 years age, in 1:1 ratio, receive QIV or hepatitis A (control). The primary end point was confirmed by real-time polymerase chain reaction (rt-PCR). Secondary points were...
The Global Vaccine Action Plan 2011–2020 (GVAP) was developed to realize the ambitions of Decade Vaccines – that all individuals and communities enjoy lives free from vaccine-preventable diseases. It included a comprehensive monitoring evaluation/accountability framework assess progress towards global targets with recommendations for corrective actions. While many GVAP are very unlikely be met by end 2020, substantial has nevertheless been made, establishing strong foundation successor...
Treatment of shigellosis in rabbits with butyrate reduces clinical severity and counteracts the downregulation cathelicidin (CAP-18) large intestinal epithelia. Here, we aimed to evaluate whether can be used as an adjunct antibiotics treatment patients. A randomized, double-blind, placebo-controlled, parallel-group designed trial was conducted. Eighty adult patients were randomized either Intervention group (butyrate, n = 40) or Placebo (normal saline, 40). The given enema containing sodium...
Summary Eighty‐nine T 4‐like phages from our phage collection were tested against four collections of childhood diarrhoea‐associated E scherichia coli isolates representing different geographical origins ( M exico versus B angladesh), serotypes (69 O , 27 H serotypes), pathotypes ETEC EPEC EIEC EAEC VTEC S higella ), epidemiological settings (community and hospitalized diarrhoea) years isolation. With a cocktail consisting 3 to 14 phages, we achieved 54% 69% coverage predominantly exico, 30%...
ABSTRACT Helicobacter pylori virulence determinants have not previously been studied in detail Latin Americans with H. infections. We characterized the vacA (vacuolating cytotoxin gene A) and cagA (cytotoxin-associated types of more than 400 single-colony isolates from 20 patients Mexico City. For 17 strains two or different genotypes were isolated gastric biopsy specimens, indicating infection . The most frequent genotype was s1b/m1. diversity marked that described previously, seven had...
From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 identified as jejuni 5 coli. The presence of flaA (100%), cadF racR dnaJ pldA ciaB (95%), virB11 (0%), ceuE (82.5%), cdtA (97.5%), cdtB cdtC wlaN (7.5%) genes was detected in C. PCR. All but one produced cytolethal distending toxin a HeLa cell assay.
Background Rapid and reliable diagnostic assays for enteric (typhoid paratyphoid) fever are urgently needed. We report the characterization of novel approach utilizing lymphocyte secretions, diagnosing patients with by TPTest procedure. Methodology detects Salmonella-specific IgA responses in culture supernatant. utilized suspected fever, other illnesses, healthy controls. also evaluated simplified modifications adaptation laboratories limited facilities equipment. Principal Findings was...
Background Rotavirus vaccines are now globally recommended by the World Health Organization (WHO), but in early 2009 WHO’s Strategic Advisory Group of Experts on Immunization reviewed available data and concluded that there was no evidence for efficacy or effectiveness a two-dose schedule human rotavirus vaccine (HRV; Rotarix) given at 6 10 wk age. Additionally, programmatic vaccination, including possible indirect effects, has not been assessed low-resource populations Asia. Methods...
There are more vaccines in development, against a greater number of pathogens, than ever before. A challenge with this exceptional level activity and investment is how to select resource the most promising approaches have significant impact on public health. The WHO Product Development for Vaccines Advisory Committee (PDVAC) was established 2014 provide strategic advice recommendations clinical development that could health low middle income countries. On 7–9th September 2015, PDVAC convened...
In December 2019 a novel coronavirus (SARS-CoV-2) that is causing the current COVID-19 pandemic was identified in Wuhan, China. Many questions have been raised about its origin and adaptation to humans. present work we performed genetic analysis of Spike glycoprotein (S) SARS-CoV-2 other related coronaviruses (CoVs) isolated from different hosts order trace evolutionary history this protein Based on sequence S gene, suggest result recombination events between bat pangolin CoVs. The hybrid...
In December 2019, the first cases of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were identified in city Wuhan, China. Since then, it has spread worldwide with new mutations being reported. The aim present study was to monitor changes genetic diversity and track non-synonymous substitutions (dN) that could be implicated fitness SARS-CoV-2 its different regions between 2019 November 2020. We analyzed 2213 complete genomes from six geographical worldwide, which...
In many countries, M1 strains of the human pathogenic bacterium group A Streptococcus are most common serotype recovered from patients with invasive disease episodes. Strains this express an extracellular protein that inhibits complement [streptococcal inhibitor (Sic)] and is therefore believed to be a virulence factor. Comparative sequence analysis 915-bp sic gene in 165 organisms diverse localities infection types identified 62 alleles. Inasmuch as multilocus enzyme electrophoresis...
Salmonella enterica serotype Paratyphi A is a human-restricted cause of paratyphoid fever, accounting for up to fifth all cases enteric fever in Asia.In this work, we applied an RNA analysis method, Selective Capture Transcribed Sequences (SCOTS), and cDNA hybridization-microarray technology identify S. transcripts expressed by bacteria the blood three patients Bangladesh. In total, detected 1,798 mRNAs infected humans (43.9% ORFeome). Of these, identified 868 at least two patients, 315...
Background There has been no population-based study on human papillomavirus (HPV) prevalence or its genotypes in Bangladesh; a country eligible for GAVI funding HPV vaccine. Methods We used baseline survey data of prospective cohort that was conducted one urban and rural area Bangladesh. A total 997 905 married women, aged 13 to 64 years, were enrolled the during July-December, 2011. Information collected socio-demographic characteristics potential risk factors infection followed by...
The Manual for Surveillance of Events Supposedly Attributable to Vaccination or Immunization in the Region Americas represented one first steps toward building regional system surveillance events supposedly attributable vaccination immunization (ESAVIs) and adverse special interest (AESIs). This manual establishes that, after notification investigation an event, a national committee experts should classify event accordance with World Health Organization (WHO) causality classification. Pan...