A5036497036- Glycosylation and Glycoproteins Research
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Polysaccharides and Plant Cell Walls
- Monoclonal and Polyclonal Antibodies Research
- Biofuel production and bioconversion
- Lipid metabolism and disorders
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Sirtuins and Resveratrol in Medicine
- Fatty Acid Research and Health
- Muscle Physiology and Disorders
- Retinoids in leukemia and cellular processes
- Enzyme Production and Characterization
- Pharmacological Effects of Medicinal Plants
- Antioxidant Activity and Oxidative Stress
- Garlic and Onion Studies
- Infant Nutrition and Health
- Radiopharmaceutical Chemistry and Applications
- Plant nutrient uptake and metabolism
- Polysaccharides Composition and Applications
- Morinda citrifolia extract uses
- Lipid metabolism and biosynthesis
University of Georgia
2014-2023
Dairy Management
2010
Jeonbuk National University
1995-2007
Abstract Resveratrol, a phytoallexin, has recently been reported to slow aging by acting as sirtuin activator. Resveratrol also wide range of pharmacological effects on adipocytes. In this study, we investigated the resveratrol adipogenesis and apoptosis using 3T3‐L1 cells. mature adipocytes, 100 200 µM decreased cell viability dose‐dependently 23 ± 2.7%, 75.3 2.8% ( p < 0.0001), respectively, after 48 h treatment, increased 76 8.7% 0.0001). at 25 50 lipid accumulation in maturing...
The natural compounds genistein (G), quercetin (Q), and resveratrol (R) have been reported to each exhibit anti-adipogenic activities in adipocytes antiproliferative pro-apoptotic several cell types. We studied the combined effects of G, Q, R on adipogenesis apoptosis primary human (HAs) 3T3-L1 murine adipocyte (MAs). Combined treatment with 6.25 μM 12.5 during 14-day differentiation period caused an enhanced inhibition lipid accumulation maturing HAs that was greater than responses...
Structural and functional characterization of the extracellular domain human CaSR with bound Mg 2+ a tryptophan derivative.
Abstract Human Milk Oligosaccharides (HMOs) are abundant carbohydrates fundamental to infant health and development. Although these oligosaccharides were discovered more than half a century ago, their biosynthesis in the mammary gland remains largely uncharacterized. Here, we use systems biology framework that integrates glycan RNA expression data construct an HMO biosynthetic network predict glycosyltransferases involved. To accomplish this, models describing most likely pathways for...
Invasive microbes causing diseases such as sudden oak death negatively affect ecosystems and economies around the world. The deployment of resistant genotypes for combating introduced typically relies on breeding programs that can take decades to complete. To demonstrate how this process be accelerated, we employed a genome-wide association mapping ca . 1,000 resequenced Populus trichocarpa trees individually challenged with Sphaerulina musiva , an invasive fungal pathogen. Among significant...
Pectin is a vital component of the plant cell wall and provides molecular glue that maintains cell-cell adhesion, among other functions. As most complex polysaccharide, pectin composed several covalently linked domains, such as homogalacturonan (HG) rhamnogalacturonan I (RG I). has widespread uses in food industry emerging biomedical applications, but its synthesis remains poorly understood. For instance, enzymes catalyze RG elongation remain unknown. Recently, coexpression- sequence-based...
Insights into the evolution of plant cell walls have important implications for comprehending these diverse and abundant biological structures. In order to understand evolving structure-function relationships wall, it is imperative trace origin its different components. The present study focused on 1,4-β-xylan, tracing evolutionary by genome transcriptome mining followed phylogenetic analysis, utilizing a large selection plants algae. It substantiates findings heterologous expression...
Abstract During circulation in humans and natural selection to escape antibody recognition for decades, A/H3N2 influenza viruses emerged with altered receptor specificities. These lost the ability agglutinate erythrocytes critical antigenic characterization give low yields acquire adaptive mutations when cultured eggs cells, contributing recent vaccine challenges. Examination of specificities reveals that compensated decreased binding prototypic human by recognizing α2,6-sialosides on...
The mechanistic underpinnings of the complex process plant polysaccharide biosynthesis are poorly understood, largely because resistance glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes regiospecific transfer terminal 1,2-fucosyl residues xyloglucan side chains - key step in fucosylated sidechains galactoxyloglucan. We unravel basis for fucosylation by AtFUT1 with...
Immunoglobulin G (IgG) is a major effector molecule of the human immune response, and aberrations in IgG glycosylation are linked to various diseases. However, molecular mechanisms underlying protein still poorly understood. We present data-driven approach infer reactions pathway using large-scale mass-spectrometry measurements. Gaussian graphical models used construct association networks from four cohorts. find that glycan pairs with high partial correlations represent enzymatic known...
Xylans are a major component of plant cell walls. O-Acetyl moieties the dominant backbone substituents glucuronoxylan in dicots and play role polymer-polymer interactions that crucial for wall architecture normal development. Here, we describe biochemical, structural, mechanistic characterization Arabidopsis (Arabidopsis thaliana) xylan O-acetyltransferase 1 (XOAT1), member plant-specific Trichome Birefringence Like (TBL) family. Detailed XOAT1-catalyzed reactions by real-time NMR confirms...
Homogalacturonan (HG) is a pectic glycan in the plant cell wall that contributes to growth and development structure function, interacts with other glycans proteoglycans wall. HG synthesized by galacturonosyltransferase (GAUT) gene family. Two members of this family, GAUT1 GAUT7, form heteromeric enzyme complex Arabidopsis thaliana Here, we established heterologous GAUT expression system HEK293 cells show co-expression recombinant GAUT7 results production soluble GAUT1:GAUT7 catalyzes...
Abstract Objective: To determine the effects of esculetin, a plant phenolic compound with apoptotic activity in cancer cells, on 3T3‐L1 adipocyte apoptosis and adipogenesis. Research Methods Procedures: pre‐confluent preadipocytes lipid‐filled adipocytes were incubated esculetin (0 to 800 μM) for up 48 hours. Viability was determined using Cell Titer 96 Aqueous One Solution cell proliferation assay; quantified by measurement single‐stranded DNA. Post‐confluent 6 days during maturation....
Abstract Objective : Determine the biochemical pathways involved in induction of apoptosis by ajoene, an organosulfur compound from garlic. Research Methods and Procedures Mature 3T3‐L1 adipocytes were incubated with ajoene at concentrations up to 200 μM. Viability quantified using MTS‐based cell viability assay enzyme‐linked immunosorbent for single‐stranded DNA (ssDNA), respectively. Intracellular reactive oxygen species (ROS) production was measured based on fluorescent dye,...
Objective: To determine the effects of guggulsterone (GS), active substance in guggulipid, on apoptosis, adipogenesis, and lipolysis using 3T3‐L1 cells. Methods Procedures: For apoptosis experiments, mature adipocytes were treated with GS isomers. Viability, caspase 3/7 activation quantified MTS, enzyme‐linked immunosorbent assay (ELISA), caspase‐Glo activity assay, respectively. The expression cytochrome c was demonstrated by western blot. Lipolysis measuring release glycerol. adipogenesis...
Beta-adrenergic activation stimulates uncoupling protein 1 (UCP1), enhancing metabolic rate. In vitro, most work has studied brown adipocytes, however, few have investigated more established adipocyte lines such as the murine 3T3-L1 line. To assess effect of beta-adrenergic activation, mature 3T3-L1s were treated for 6 or 48 hours with without isoproterenol (10 and 100 μM) following standard differentiation supplemented thyroid hormone (T3; nM). The highest dose increased lipid content...