A5014885625- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Sirtuins and Resveratrol in Medicine
- Hops Chemistry and Applications
- Pharmacological Effects of Medicinal Plants
- Fatty Acid Research and Health
- Bone Metabolism and Diseases
- Phytochemicals and Antioxidant Activities
- PI3K/AKT/mTOR signaling in cancer
- Regulation of Appetite and Obesity
- Exercise and Physiological Responses
- Toxin Mechanisms and Immunotoxins
- Immune Response and Inflammation
- Genomics, phytochemicals, and oxidative stress
- Infant Nutrition and Health
- SARS-CoV-2 and COVID-19 Research
- Phytoestrogen effects and research
- Vitamin D Research Studies
- Diet and metabolism studies
- Natural Antidiabetic Agents Studies
- Antioxidant Activity and Oxidative Stress
- Pharmacological Effects of Natural Compounds
- Bee Products Chemical Analysis
- Liver Disease Diagnosis and Treatment
- Fungal Plant Pathogen Control
Philadelphia College of Osteopathic Medicine
2016-2025
University of Georgia
2005-2012
Dairy Management
2010
Abstract Resveratrol, a phytoallexin, has recently been reported to slow aging by acting as sirtuin activator. Resveratrol also wide range of pharmacological effects on adipocytes. In this study, we investigated the resveratrol adipogenesis and apoptosis using 3T3‐L1 cells. mature adipocytes, 100 200 µM decreased cell viability dose‐dependently 23 ± 2.7%, 75.3 2.8% ( p < 0.0001), respectively, after 48 h treatment, increased 76 8.7% 0.0001). at 25 50 lipid accumulation in maturing...
Emerging outbreak of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is a major threat to public health. The morbidity increasing due lack SARS-CoV-2 specific drugs. Herein, we have identified potential drugs that target the 3-chymotrypsin like protease (3CLpro), main pivotal for replication SARS-CoV-2. Computational molecular modeling was used screen 3987 FDA approved drugs, and 47 were selected study their inhibitory effects on 3CLpro enzyme in vitro. Our results...
The natural compounds genistein (G), quercetin (Q), and resveratrol (R) have been reported to each exhibit anti-adipogenic activities in adipocytes antiproliferative pro-apoptotic several cell types. We studied the combined effects of G, Q, R on adipogenesis apoptosis primary human (HAs) 3T3-L1 murine adipocyte (MAs). Combined treatment with 6.25 μM 12.5 during 14-day differentiation period caused an enhanced inhibition lipid accumulation maturing HAs that was greater than responses...
Abstract Both central and peripheral leptin administrations reduce body weight, food intake, adiposity in ob/ob mice. In this study we compared effects of intracerebroventricular (ICV) subcutaneous (SC) administration on bone metabolism the appendicular axial skeleton adipose tissue gene expression determined ICV marrow experiment 1, (1.5 or 0.38 µg/d) control was continuously injected for 12 days. Gene analysis femoral stromal cells showed that genes associated with osteogenesis increased...
Abstract The most common host entry point of human adapted coronaviruses (CoV) including SARS-CoV-2 is through the initial colonization in nostril and mouth region which responsible for spread infection. Most recent studies suggest that commercially available oral nasal rinse products are effective inhibiting viral replication. However, anti-viral mechanism active ingredients present rinses have not been studied. In study, we assessed vitro enzymatic inhibitory activity products: aloin A B,...
Obesity alters adipose tissue metabolic and endocrine functioning, leading to an increased adiposity release of pro-inflammatory cytokines. Various phytochemicals have been reported contribute the beiging white in order ameliorate obesity by increasing thermogenesis. Here, we show that prenylated chalcone, xanthohumol (XN), induces adipocytes, stimulates lipolysis, inhibits adipogenesis murine 3T3-L1 adipocytes primary human subcutaneous preadipocytes these effects are partly mediated...
The 3-chymotrypsin-like protease (3CLpro), papain-like (PLpro), and RNA-dependent RNA polymerase (RdRp) are key enzymes in SARS-CoV-2 replication serve as critical targets for an antiviral drug. Currently, Paxlovid® Lagevrio™ specifically target 3CLpro RdRp, respectively, COVID-19 treatment. However, no antivirals the PLpro enzyme, essential viral suppression of host immune response. This study identified 9-aminominocycline (9-AMN) a potent inhibitor PLpro. Unlike parent compound...
Beta-adrenergic activation stimulates uncoupling protein 1 (UCP1), enhancing metabolic rate. In vitro, most work has studied brown adipocytes, however, few have investigated more established adipocyte lines such as the murine 3T3-L1 line. To assess effect of beta-adrenergic activation, mature 3T3-L1s were treated for 6 or 48 hours with without isoproterenol (10 and 100 μM) following standard differentiation supplemented thyroid hormone (T3; nM). The highest dose increased lipid content...
Abstract This paper describes effects of several sulfur‐containing compounds from garlic on the cell viability, apoptosis and adipogenesis in 3T3‐L1 adipocytes. In both preadipocytes mature adipocytes, 100 200 µM ajoene significantly decreased viability increased apoptosis. The effect was further confirmed with Hoechst staining. contrast, diallyl sulfide, disulfide, trisulfide, deoxyalliin, allyl methyl sulfide had no significant or either maturing lipid accumulation a dose‐dependent manner...
Objective: To investigate the ability of 1,25(OH) 2 D 3 (D) and genistein (G), alone in combination, to inhibit adipogenesis induce apoptosis 3T3‐L1 adipocytes. Methods Procedures: preadipocytes mature adipocytes were incubated with various concentrations G, for 48 h. Viability was determined using Cell Titer 96 Aqueous One Solution Proliferation Assay. Post‐confluent G up 6 days during lipid content quantified by Nile Red dye; measurement single‐stranded DNA. Expression adipocyte‐specific...
Vitamin D and certain natural compounds have been shown to regulate both lipid metabolism bone formation. Treatments that prevent or reverse age-related increase in marrow adiposity could new formation inhibit destruction. We tested the hypothesis dietary supplementation with combinations of vitamin phytochemicals inhibits loss decreases a greater extent than control D-alone diets. Aged ovariectomized female rats (12 months old, n=50, initial body weight=240 g) were given (AIN-93M diet),...
Xanthohumol (XN) and guggulsterone (GS) have each been shown to inhibit adipogenesis induce apoptosis in adipocytes. In the present study effects of combination XN + GS on 3T3-L1 adipocyte were investigated. Mature adipocytes treated with individually combination. decreased cell viability, but caused an enhanced decrease viability potentiated induction apoptosis. Likewise, a increase caspase-3/7 activation, whereas neither compounds showed any effect individually. addition, western blot...