A5005017303- Airway Management and Intubation Techniques
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Cardiac, Anesthesia and Surgical Outcomes
- Anesthesia and Sedative Agents
- Auditing, Earnings Management, Governance
- Respiratory Support and Mechanisms
- Corporate Finance and Governance
- Anesthesia and Neurotoxicity Research
- Financial Reporting and Valuation Research
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Medical History and Innovations
- Salivary Gland Disorders and Functions
- Metabolomics and Mass Spectrometry Studies
- Monoclonal and Polyclonal Antibodies Research
- Tracheal and airway disorders
- Protein purification and stability
- Congenital Diaphragmatic Hernia Studies
- Cardiac Arrest and Resuscitation
- Analytical Chemistry and Chromatography
- Anesthesia and Pain Management
- Foreign Body Medical Cases
- Cassava research and cyanide
- Diversity and Career in Medicine
University of California, San Francisco
2010-2024
Broad Center
2021-2024
Life Sciences Discovery Fund
2023-2024
Liverpool School of Tropical Medicine
2024
City College of San Francisco
2021
Reproductive Science Center
2014-2021
Stony Brook University
1996-2021
Universidade Federal de Goiás
2020
University of Nebraska at Kearney
1996-2019
Novartis (United States)
2017
Saliva is a body fluid with important functions in oral and general health. A consortium of three research groups catalogued the proteins human saliva collected as ductal secretions: 1166 identifications--914 parotid 917 submandibular/sublingual saliva--were made. The results showed that high proportion are found plasma and/or tears also present along unique components. identified involved numerous molecular processes ranging from structural to enzymatic/catalytic activities. As expected,...
There is an increasing need in biology and clinical medicine to robustly reliably measure tens hundreds of peptides proteins biological samples with high sensitivity, specificity, reproducibility, repeatability. Previously, we demonstrated that LC-MRM-MS isotope dilution has suitable performance for quantitative measurements small numbers relatively abundant human plasma the resulting assays can be transferred across laboratories while maintaining reproducibility precision. Here,...
The All of Us (AoU) initiative aims to sequence the genomes over one million Americans from diverse ethnic backgrounds improve personalized medical care. In a recent technical pilot, we compare performance traditional short-read sequencing with long-read in small cohort samples HapMap project and two AoU control representing eight datasets. Our analysis reveals substantial differences ability these technologies accurately complex medically relevant genes, particularly terms gene coverage...
APOBEC3G (A3G) and related deoxycytidine deaminases are potent intrinsic antiretroviral factors. A3G is expressed either as an enzymatically active low-molecular-mass (LMM) form or inactive high-molecular-mass (HMM) ribonucleoprotein complex. Resting CD4 T cells exclusively express LMM A3G, where it functions a powerful postentry restriction factor for HIV-1. Activation of promotes the recruitment into 5- to 15-MDa HMM complexes whose function unknown. Using tandem affinity purification...
We report a general mass spectrometric approach for the rapid identification and characterization of proteins isolated by preparative two-dimensional polyacrylamide gel electrophoresis. This method possesses inherent power to detect structurally characterize covalent modifications. Absolute sensitivities matrix-assisted laser desorption ionization high-energy collision-induced dissociation tandem spectrometry are exploited determine sequence subpicomole sample quantities tryptic peptides....
Multiple reaction monitoring (MRM) mass spectrometry coupled with stable isotope dilution (SID) and liquid chromatography (LC) is increasingly used in biological clinical studies for precise reproducible quantification of peptides proteins complex sample matrices. Robust LC-SID-MRM-MS-based assays that can be replicated across laboratories ultimately laboratory settings require standardized protocols to demonstrate the analysis platforms are performing adequately. We developed a system...
Saliva plays many biological roles, from lubrication and digestion to regulating bacterial leukocyte adhesion. To understand the functions of individual components families molecules, it is important identify as salivary proteins possible. Toward this goal, we used a proteomic approach first step in global analysis body fluid. We collected parotid saliva ductal secretion three human donors separated protein by two-dimensional SDS-polyacrylamide gel electrophoresis (2D SDS−PAGE). Proteins...
Changes in salivary composition correlate with disease susceptibility, state, or both. However, use of saliva for diagnostic purposes is complicated by the gland-specific effects circadian rhythm diurnal variation. We recently characterized a suite peptides < =10-kDa fraction human parotid that included many novel species. In this study, we used iTRAQ labeling chemistry to investigate possible on peptide generation. collected samples produced gustatory stimulation as ductal secretions at...
Abstract The All of Us (AoU) initiative aims to sequence the genomes over one million Americans from diverse ethnic backgrounds improve personalized medical care. In a recent technical pilot, we compared performance traditional short-read sequencing with long-read in small cohort samples HapMap project and two AoU control representing eight datasets. Our analysis revealed substantial differences ability these technologies accurately complex medically relevant genes, particularly terms gene...
Background Recent studies have determined that an initial rectal acetaminophen dose of approximately 40 mg/kg is needed in children to achieve target antipyretic serum concentrations. The timing and amount subsequent doses after a 40-mg/kg has not been clarified for this route administration. Based on the authors' previous pharmacokinetic data, they examined whether loading followed by 20-mg/kg at 6-h intervals maintain concentrations within range 10-20 microg/ml, without evidence...
Przybylo, H. J. MD; Stevenson, G. W. Backer, Carl Luck, Susan R. Webb, Catherine L. Morgan, Elaine Hall, Steven C. MD Author Information
We have integrated preparative two-dimensional polyacrylamide gel electrophoresis with high-performance tandem mass spectrometry and Edman degradation. By using this approach, we isolated identified, by partial sequencing, a human melanoma protein (34 kDa, pI 6.4) as lipocortin I. To our knowledge, was not previously known to be associated cells. The identity of the confirmed immunoblot analysis. High-energy collision-induced dissociation analysis revealed sequence acetylation N-terminal...
We used a lectin chromatography/MS-based approach to screen conditioned medium from panel of luminal (less aggressive) and triple negative (more breast cancer cell lines (n = 5/subtype). The samples were fractionated using the lectins Aleuria aurantia (AAL) Sambucus nigra agglutinin (SNA), which recognize fucose sialic acid, respectively. bound fractions enzymatically N-deglycosylated analyzed by LC–MS/MS. In total, we identified 533 glycoproteins, ∼90% components surface or extracellular...
Desorption/ionization on silicon (DIOS) tandem time-of-flight (TOF/TOF) mass spectrometry (MS) provides high accuracy and significant fragmentation information, particularly in the characterization of biomolecules. DIOS TOF/TOF offers a high-throughput surface-based ionization platform as well complete through collision energies. The absence matrix interference allows for MS MS/MS analysis small molecules below m/z 300. In addition, sample preparation is minimal, chips can be stored...
A strategy for the identification of site occupancy and glycoform heterogeneity, including sialylation occurring at specific sites N-linked giycoproteins is presented using asparagine-linked glycosylation on bovine fetuin illustration. This achieved by microbore high-performance liquid chromatography/electrospray ionization mass analysis (LC/ESIMS) tryptic glycopeptide mixtures with an acetonitrile-based mobile phase followed sequential steps residue (and linkage) degradation LC/ESIMS each...
Apolipoprotein E (apoE) is a major protein component of cholesterol-transporting lipoprotein particles in the central nervous system and plasma. Polymorphisms apoE are associated with cardiovascular disease predisposition to Alzheimer's other forms neurodegeneration. For full biological activity, must be bound particle. Complexes phospholipid mimic many these activities. In contrast widely accepted discoidal model apoA-I dimyristoylphosphatidylcholine, which based on solution studies, an...
ABSTRACT Bartonella quintana is a fastidious, gram-negative, rod-shaped bacterium that causes prolonged bacteremia in immunocompetent humans and severe infections immunocompromised individuals. We sought to define the outer membrane subproteome of B. order obtain insight into biology pathogenesis this emerging pathogen identify predominant antigens targeted by human immune system during infection. isolated total proteins identified 60 two-dimensional sodium dodecyl sulfate-polyacrylamide gel...
In enzymatic 18O-labeling strategies for quantitative proteomics, the exchange of carboxyl oxygens at low pH is a common, undesired side reaction. We asked if acid-catalyzed back could interfere with quantitation and whether reaction itself be used as method introducing 18O label into peptides. Several synthetic peptides were dissolved in dilute acid containing 50% (v/v) H218O incubated room temperature. Aliquots removed over period 3 weeks analyzed by tandem mass spectrometry (MS/MS)....
Tandem affinity purification is the principal method for purifying and identifying stable protein complexes system-wide in whole cells. Although highly effective, this approach laborious impractical organisms where genetic manipulation not possible. Here, we propose a novel "tagless" strategy that combines multidimensional separation of endogenous with mass spectrometric monitoring their composition. In procedure, putative are identified based on comigration collections polypeptides through...
Extensive clinical experience and many studies support the use of IV patient-controlled analgesia (IV PCA) regional anesthesia techniques for treatment postoperative pain in children. In contrast, little has been reported about ability children to epidural (PCEA) or efficacy this technique. We report a descriptive analysis prospectively recorded data 128 (132 procedures) whom PCEA was used acute control. Satisfactory obtained 119 patients (90.1%) up 103 h with no episodes desaturation...