A5080250443- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Meat and Animal Product Quality
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Horticultural and Viticultural Research
- Advanced Proteomics Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Identification and Quantification in Food
- Diet and metabolism studies
- Genetic Mapping and Diversity in Plants and Animals
- Mitochondrial Function and Pathology
- Pesticide Residue Analysis and Safety
- Legume Nitrogen Fixing Symbiosis
- Plant Physiology and Cultivation Studies
- Fermentation and Sensory Analysis
- Mycobacterium research and diagnosis
- Pharmacological Effects and Assays
- Tuberculosis Research and Epidemiology
- Atmospheric chemistry and aerosols
- Hops Chemistry and Applications
- Genomics and Chromatin Dynamics
- Gut microbiota and health
- Postharvest Quality and Shelf Life Management
- Soil Carbon and Nitrogen Dynamics
Colorado State University
2016-2025
Proteogenomics Research Institute for Systems Medicine
2010
University of Wyoming
2010
Scripps Research Institute
2003
Analyses of circulating metabolites in large prospective epidemiological studies could lead to improved prediction and better biological understanding coronary heart disease (CHD). We performed a mass spectrometry-based non-targeted metabolomics study for association with incident CHD events 1,028 individuals (131 events; 10 y. median follow-up) validation 1,670 (282 3.9 follow-up). Four were replicated independent main cardiovascular risk factors [lysophosphatidylcholine 18∶1 (hazard ratio...
Metabolomic data are frequently acquired using chromatographically coupled mass spectrometry (MS) platforms. For such datasets, the first step in analysis relies on feature detection, where a is defined by and retention time. While typically derived from single compound, spectrum of signals more more-accurate representation spectrometric signal for given metabolite. Here, we report novel grouping method that operates an unsupervised manner to group MS into spectra without relying...
Background Mycobacterium tuberculosis (Mtb), the causative agent of (TB), is a facultative intracellular pathogen that can persist within host. The bacteria are thought to be in state reduced replication and metabolism as part chronic lung infection. Many vitro studies have dissected hypothesized environment infected lung, defining bacterial response pH, starvation hypoxia. While these experiments afforded great insight, picture remains incomplete. only way study combined effects...
Abstract It has long been known that precipitation can impact atmospheric aerosol, altering number concentrations and size‐dependent composition. Such effects result from competing mechanisms: remove particles through wet deposition, or lead to the emission of mechanical ejection, biological processes, re‐suspension associated wind gusts. These feed back into hydrologic cycle by serving as cloud nuclei. In this study, we investigated how at a forested site impacted concentration composition...
Emerging evidence supporting chronic disease fighting properties of rice bran has advanced the development stabilized for human use as a functional food and dietary supplement. A global targeted metabolomic investigation fermented with Saccharomyces boulardii was performed in three varieties. Metabolites from S. boulardii-fermented were detected by gas chromatography−mass spectrometry (GC−MS) assessed bioactivity compared to nonfermented normal malignant lymphocytes. Global metabolite...
Metabolomics is an emerging method to improve our understanding of how genetic diversity affects phenotypic variation in plants. Recent studies have demonstrated that genotype has a major influence on biochemical several types plant tissues, however, the association between metabolic and morphological physiological traits largely unknown. Sorghum bicolor (L.) important food fuel crop with extensive variation. lines been bred for differing phenotypes beneficial production grain (food), stem...
Liquid chromatography coupled to electrospray ionization-mass spectrometry (LC-ESI-MS) is a versatile and robust platform for metabolomic analysis. However, while ESI soft ionization technique, in-source phenomena including multimerization, nonproton cation adduction, fragmentation complicate interpretation of MS data. Here, we report chromatographic mass spectrometric behavior 904 authentic standards collected under conditions identical typical nontargeted profiling experiment. The data...
Abstract Ambient mass spectrometry is an analytical approach that enables ionization of molecules under open-air conditions with no sample preparation and very fast sampling times. Rapid evaporative (REIMS) a relatively new type ambient has demonstrated applications in both human health food science. Here, we present evaluation REIMS as tool to generate molecular scale information objective measure for the assessment beef quality attributes. Eight different machine learning algorithms were...
Decrypting the chemical interactions between plant roots and soil microbiome is a gateway for future manipulation management of rhizosphere, compartment critical to promoting fitness yields. Our experimental results demonstrate how microbial community genomic diversity influenced by root exudates differing compositions changes in this result altered production plant-relevant metabolites.
ABSTRACT Multidrug-resistant tuberculosis has emerged as a major threat to control. Phylogenetically related rifampin-resistant actinomycetes with mutations mapping clinically dominant Mycobacterium in the rpoB gene show upregulation of networks encoding secondary metabolites. We compared expressed proteomes and metabolomes two fully drug-susceptible clinical strains M. (wild type) those their respective rifampin-resistant, mutant progeny confirmed rifampin monoresistance following...
The H1 linker histones are abundant chromatin-associated DNA-binding proteins. Recent evidence suggests that also may function through protein–protein interactions. To gain a better understanding of the scope histone involvement in interactions, we used proteomics approach to identify H1-binding proteins human nuclear extracts. Full-length H1.0 and lacking its C-terminal domain (CTD) were for protein pull-downs. A total 107 candidate binding identified by LC-MS/MS. About one-third...
Interrelationship between mitochondria and myoglobin function influence beef color. NADH level in postmortem muscle is an important determinant of mitochondrial activity metmyoglobin reduction. Increased aging time promotes discoloration steaks; however, the mechanism this effect not clear. The objective was to characterize role wet-aging longissimus lumborum global metabolome determine that can regenerate NADH. Beef muscles were randomly assigned 3, 7, 14, 21, 28 days periods. Surface...
Microgreens are an emerging functional food crop with promise for sustainably diversifying global systems, facilitating adaptations to urbanization and climate change, promoting human health. Previous work suggests microgreens have high nutritional quality, low environmental impacts, broad consumer acceptance. For better reception into the system increased per capita consumption, research is needed elucidate acceptance of various species, including factors contributing their or lack thereof....
Globally, we are failing to meet numerous nutritional, health, and environmental targets linked food. Defining food composition in its full chemical quantitative diversity is central data-driven decision making for supporting nutrition sustainable diets. "Foodomics"-the application of omics-technology characterize quantify biomolecules improve wellbeing-has the potential comprehensively elucidate what food, how this varies across system, diet as an ensemble foods guides outcomes nutrition,...
<title>Abstract</title> Background Cover cropping is an agricultural practice that uses secondary crops to support the growth of primary through various mechanisms including erosion control, weed suppression, nutrient management, and enhanced biodiversity. may elicit some these ecosystem services chemical interactions with soil microbiome via root exudation, or release plant metabolites from roots. Phytohormones are one metabolite type exuded by plants activate rhizosphere microbiome, yet...
The complex cell wall of Mycobacterium tuberculosis is the hallmark acid fast bacteria and responsible for much its physiological characteristics. Hence, effort has been made to determine primary structure. Such studies have hampered by extreme complexity. Also, insolubility leads difficulties determining presence or absence base labile groups. We used an endogenous arabinase solubilize arabinan region shown using mass spectrometry NMR that succinyl esters are present on O2 inner-branched...