A5029734068- Oral microbiology and periodontitis research
- Salivary Gland Disorders and Functions
- HIV/AIDS oral health manifestations
- HIV Research and Treatment
- Biosensors and Analytical Detection
- Reproductive tract infections research
- Herpesvirus Infections and Treatments
- Streptococcal Infections and Treatments
- HIV/AIDS Research and Interventions
- Microfluidic and Capillary Electrophoresis Applications
- Mosquito-borne diseases and control
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- Cervical Cancer and HPV Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Oral Health Pathology and Treatment
- HIV/AIDS drug development and treatment
- Advanced Drug Delivery Systems
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- HIV-related health complications and treatments
- Microbial Community Ecology and Physiology
- Advanced Biosensing Techniques and Applications
- Pelvic floor disorders treatments
- Reproductive System and Pregnancy
New York University
2009-2018
Hebrew University of Jerusalem
2017
NYU Langone Health
2016
University School
2015
New York College of Health Professions
2015
Indiana University School of Medicine
2009
GTx (United States)
2009
American Association of Immunologists
2008
University of Rochester Medical Center
2008
University of Southern California
2008
AIM:To design and validate broad-range 16S rRNA primers for use in high throughput sequencing to classify bacteria isolated from the human foregut microbiome.
Saliva is a body fluid with important functions in oral and general health. A consortium of three research groups catalogued the proteins human saliva collected as ductal secretions: 1166 identifications--914 parotid 917 submandibular/sublingual saliva--were made. The results showed that high proportion are found plasma and/or tears also present along unique components. identified involved numerous molecular processes ranging from structural to enzymatic/catalytic activities. As expected,...
The proteome of human salivary fluid has the potential to open new doors for disease biomarker discovery. A recent study comprehensively identify and catalog ductal led compilation 1166 proteins. protein complexity both saliva plasma is large, suggesting that a comparison these two proteomes will provide valuable insight into their physiological significance an understanding unique overlapping diagnostic each provides. To create more comprehensive proteins, we have first compiled extensive...
A leukotoxin from Actinobacillus actinomycetemcomitans was isolated by a procedure that includes polymyxin B extraction, ion-exchange chromatography, and gel filtration chromatography. The resulted in the recovery of 48% toxin with 99-fold increase specific activity. has molecular mass 180,000 daltons 115,000 sodium dodecyl sulfate-polyacrylamide electrophoresis. It retains all major biological characteristics previously documented for crude preparations, including susceptibility to heat...
A simple, point of care, inexpensive, disposable cassette for the detection nucleic acids extracted from pathogens was designed, constructed, and tested. The utilizes a single reaction chamber isothermal amplification acids. is equipped with an integrated, flow-through, Flinders Technology Associates (Whatman FTA®) membrane isolation, concentration, purification DNA and/or RNA. captured by are used directly as templates without elution, thus simplifying cassette's flow control. FTA also...
Colonization of oral tissues by Streptococcus sanguis may be influenced a mucin-like salivary glycoprotein (SAG) through calcium-dependent interaction with specific bacterial receptor. We report the nucleotide and deduced amino acid sequence S. receptor (SSP-5) show that this protein bind sialic residues SAG. The SSP-5 contains three unique structural domains, two which consist repetitive sequences. N-terminal domain is comprised four tandem copies an 82-residue repeat exhibits homology to M...
ABSTRACT Alkyl betaines and alkyl dimethylamine oxides have been shown to pronounced antimicrobial activity when used individually or in combination. Although several studies conducted with these compounds combinations, only equimolar concentrations of the C 12 /C 16 14 chain lengths for betaine amine oxide, respectively, investigated. This study investigates a wide range (C 8 18 ) both oxide attempts correlate their micelle-forming capabilities biological activity. A broth microdilution...
We previously observed that when human immunodeficiency virus (HIV)-infected T lymphocytes are added to epithelial cells, they adhere, polarize, and secrete virions unidirectionally onto the epithelium. Epithelial cells subsequently take up become productively infected. report here colchicine treatment of T-lymphocyte suspensions induced lymphocyte polarization, redistribution F-actin into a pseudopod, secretion HIV from pseudopod. Immobilization on negatively charged plastic also caused...
There have been significant advances in techniques for the detection of biomarker signals oral cavity ( e.g., ELISAs proteins, PCR RNA and DNA) as well engineering development microfluidic approaches to make oral-based point-of-care (POC) methods diagnosis both local systemic conditions a reality. In this section, we focus on three such approaches, namely, periodontal disease management, early markers diseases, salivary useful pharmacogenomic studies. Novel using non-invasive, samples...
Limited information is available about the effects of HIV and subsequent antiretroviral treatment on host-microbe interactions. This study aimed to determine salivary microbial composition for 10 HIV-seropositive subjects, before 6 months after highly active therapy (HAART), compared with that HIV-seronegative subjects. A conventional culture two culture-independent analyses were used consistently demonstrated differences in among three sets samples. HIV-positive subjects had higher levels...
Interspecies binding is important in the colonization of oral cavity by bacteria. Streptococcus mutans can adhere to other plaque bacteria, such as sanguis and Actinomyces viscosus, this adherence enhanced saliva. The salivary bacterial molecules that mediate interaction were investigated. Salivary agglutinin, a mucinlike glycoprotein known aggregation many streptococci vitro, was found S. or A. viscosus. Adherence saliva- agglutinin-coated viscosus inhibited antibodies agglutinin receptor....
ABSTRACT Sodium dodecyl sulfate (SDS), an alkyl surfactant derived from organic alcohol, possesses properties but also denatures and unfolds both monomeric subunit proteins. In preliminary experiments, we demonstrated that SDS is a potent inactivator of herpes simplex virus type 2 human immunodeficiency 1 at concentrations comparable to those used for the nonoxynol-9. We hypothesized might be capable denaturing capsid proteins nonenveloped viruses. this report, demonstrate inactivation...
A self-actuated, flow-cycling polymerase chain reaction (PCR) reactor that takes advantage of buoyancy forces to continuously circulate reagents in a closed loop through various thermal zones has been constructed, tested, and modeled. The heating required for the PCR is advantageously used induce fluid motion without need pump. Flow velocities on order millimeters per second are readily attainable. In our preliminary prototype, we measured cross-sectionally averaged velocity 2.5 mm/s cycle...