A5051856991- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Capillary Electrophoresis Applications
- Identification and Quantification in Food
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Enzyme Structure and Function
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Salivary Gland Disorders and Functions
- Bacteriophages and microbial interactions
- Analytical Chemistry and Chromatography
- Bacillus and Francisella bacterial research
- Acute Ischemic Stroke Management
- Mycotoxins in Agriculture and Food
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Antimicrobial Peptides and Activities
- Neurological Disorders and Treatments
- Microbial Community Ecology and Physiology
- Oral microbiology and periodontitis research
- S100 Proteins and Annexins
- Metabolism and Genetic Disorders
University of Illinois Hospital & Health Sciences System
2024
Centers for Disease Control and Prevention
2011-2021
National Center for Environmental Health
2020-2021
University of Notre Dame
2021
University of Alberta
2021
University of British Columbia
2021
Lund University
2021
Knoxville College
2021
University of Tennessee at Knoxville
2002-2021
Michigan State University
2021
The proteome of human salivary fluid has the potential to open new doors for disease biomarker discovery. A recent study comprehensively identify and catalog ductal led compilation 1166 proteins. protein complexity both saliva plasma is large, suggesting that a comparison these two proteomes will provide valuable insight into their physiological significance an understanding unique overlapping diagnostic each provides. To create more comprehensive proteins, we have first compiled extensive...
Abstract Recently, we have developed a high‐resolution two‐dimensional separation strategy for the analysis of complex peptide mixtures. This methodology employs isoelectric focusing peptides on immobilized pH gradient (IPG) gels in first dimension, followed by reversed‐phase chromatography second and subsequent tandem mass spectrometry analysis. The traditional approach to this mixture problem strong‐cation‐exchange (SCX) dimension. Here, present direct comparison these two...
One of the major unanswered questions in quantitative proteomics is that dynamic protein turnover cell. Here we present a new approach to measures relative proteins cellular systems. In this approach, termed synthesis/degradation ratio mass spectrometry, stable isotope labeling employed calculate reflects rate at which 13C incorporated into individual This calculation based on Poisson distribution model designed support high-throughput analysis. Protein separation and analysis accomplished...
Here we present a comprehensive method for proteome analysis that integrates both intact protein measurement ("top-down") and proteolytic fragment characterization ("bottom-up") mass spectrometric approaches, capitalizing on the unique capabilities of each method. This integrated approach was applied in preliminary proteomic Shewanella oneidensis, metal-reducing microbe potential importance to field bioremediation. Cellular lysates were examined directly by "bottom-up" as well fractionated...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of disease 2019 (COVID-19). The spike protein expressed on the surface this virus is highly glycosylated and plays an essential role during process infection. We conducted comprehensive mass spectrometric analysis N-glycosylation profiles SARS-CoV-2 proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) spectrometry. patterns within recombinant ectodomain...
Here we present the theoretical and experimental evaluation of peptide isoelectric point as a method to aid in identification peptides from complex mixtures. Predicted pI values were found match closely experimentally obtained data, resulting development unique filter that lowers effective false positive rate for identification. Due reduction rate, cross-correlation parameters Xcorr ΔCn SEQUEST program can be lowered 25% more identifications. This approach was successfully applied analysis...
ABSTRACT Unique patterns of biomarkers were reproducibly characterized by matrix-assisted laser desorption ionization (MALDI)–mass spectrometry and used to distinguish Bacillus species members from one another. Discrimination at the strain level was demonstrated for cereus spores. Lipophilic invariant in globigii spores produced three different media B. stored more than 30 years. The sensitivity less 5,000 cells deposited analysis. Protein also MALDI analysis using treated briefly with...
Immobilized lectins have now been incorporated into affinity surfaces that can be used to isolate broad classes of samples for mass spectrometric analysis. A carbohydrate and a bacterial species displays the binding motif were isolated concentrated out solutions containing salt, urea, buffers, other contaminants are deleterious MALDI spectrometry. Concanavalin was immobilized gold foil via self-assembled monolayer. Samples in phosphate buffer or urine applied capture surface allowed...
In a preliminary report (Bundy, J. L.; Fenselau, C. Anal. Chem. 1999, 71, 1460−1463), we demonstrated the use of lectin-derivatized surfaces to capture and concentrate complex carbohydrates as well microorganisms from sample matrixes unamenable direct MALDI mass spectrometry. Here, extend work include samples representative wider variety importance human health enveloped viruses. this study, lectins were immobilized directly membrane surface via primary amines. A complementary approach was...
Electron-transfer dissociation (ETD) has recently been introduced as a fragmentation method for peptide and protein analysis. Unlike collisionally induced (CID), by ETD occurs randomly along the backbone. With use of sequences determined from termini parent mass, intact proteins can be unambiguously identified. Because fast kinetics these reactions, top-down proteomics performed using in linear ion trap mass spectrometer on chromatographic time scale. Here we demonstrate utility...
N-glycosylation plays an important role in the structure and function of membrane secreted proteins. The spike protein on surface severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), virus that causes COVID-19, is heavily glycosylated major target for developing vaccines, therapeutic drugs diagnostic tests. first SARS-CoV-2 variant carries a D614G substitution (S-D614G) has been associated with altered conformation, enhanced ACE2 binding, increased infectivity transmission. In this...
Electrospray ionization combined with ion/ion reactions in a quadrupole ion trap can be used for the direct analysis of oligonucleotide mixtures. Elements to success this approach include factors related ionization, reactions, and mass analysis. This paper deals issues regarding polarity combination, viz., positive oligonucleotides/negative charge-transfer agent versus negative oligonucleotides/positive agent. Anions derived from perfluorocarbons appear directly applicable mixtures ions...
Differential labeling of peptides via the use 18O-water proteolytic method has been widely adopted for quantitative shotgun proteomics studies due to its simplicity and low reagent costs. In this report, immobilized trypsin in initial digestion step, addition is explored as a means minimize postlabeling back exchange 18O-labeled into 16O form when multidimensional peptide separation methods (here, isoelectric focusing peptides) are incorporated sample workflow. Examples shown with mixture...
Defensins are highly basic cationic peptides that important components of the innate and adaptive immune response pathways. In addition, these involved in CD8+ T cell to HIV-1, increased pulmonary infection risk among cystic fibrosis patients, upregulated levels HNP-5 for patients with ulcerative colitis Crohn's disease, monitoring HNP-3 as a tumor classification scheme cutaneous lymphomas, have promise pharmaceutical field new class antibiotics. Here we present parallel assay α (HNP1−3) β...
Viruses frequently infect the heart but clinical myocarditis is rare, suggesting that cardiac antiviral response uniquely effective. Indeed, Type I interferon (IFN) cell-type specific and provides one integrated network of protection for heart. Here, a proteomic approach was used to identify additional proteins may be involved in response. Reovirus-induced murine reflects direct viral damage cells offers an excellent system study. Primary cultures myocytes were infected with myocarditic or...
Pyrococcus furiosus is one of the most extensively studied hyperthermophilic archaea. Proteins from this hyperthemophile organism are extremely thermostable and highly resistant to chemical denaturants, organic solvents proteolytic digestion. This thermostability makes it difficult apply traditional methods enzymatically digesting a complex mixture proteins, commonly first step in peptide generation shotgun proteomics methods. Here, we have developed simple approach for global identification...
To report the first known case of intraosseous (IO) administration tenecteplase in stroke.
The performance of infrared (2.94 µm) and ultraviolet (337 nm) lasers were compared for analysis purified spores B. subtilis, cereus globigii on a four-inch end-cap reflectron time-of-flight instrument. Infrared matrix-assisted laser desorption/ionization (IR-MALDI) mass spectra these microorganisms displayed larger number biomarker peaks above m/z 4000, with UV-MALDI. Biomarker observed at higher values the IR laser. Copyright © 2000 John Wiley & Sons, Ltd.
Current guidelines for acute ischemic stroke recommend timely administration of intravascular thrombolytic therapy to promote functional and neurologic outcomes. Tenecteplase is an emerging off-label this indication being utilized by various institutions due its simpler strategy. In emergent situations in which intravenous access cannot be obtained, intraosseous a viable option medication administration. However, there has been minimal published cases support the efficacy safety tenecteplase stroke.
Abstract N-glycosylation plays an important role in the structure and function of membrane secreted proteins. The spike protein on surface severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), virus that causes COVID-19, is heavily glycosylated major target for developing vaccines, therapeutic drugs diagnostic tests. first SARS-CoV-2 variant carries a D614G substitution (S-D614G) has been associated with altered conformation, enhanced ACE2 binding, increased infectivity transmission....