A5081563992- Analytical Chemistry and Chromatography
- Protein purification and stability
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Metabolomics and Mass Spectrometry Studies
- Advanced Biosensing Techniques and Applications
- Chromatography in Natural Products
- Redox biology and oxidative stress
- Nanofabrication and Lithography Techniques
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Advanced Glycation End Products research
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Arachnid Ecology and Behavior
- Biochemical effects in animals
- Protein Structure and Dynamics
- Advanced Chemical Sensor Technologies
- Enzyme Structure and Function
- Photonic and Optical Devices
- Chemical Synthesis and Analysis
University of Liège
2024
Novilytic (United States)
2013-2023
Purdue University West Lafayette
2008-2019
National Institute of Standards and Technology
2009
Food and Drug Administration
2009
New York University
2009
NCCOS Hollings Marine Laboratory
2009
Vanderbilt University Medical Center
2009
University of Arizona
2009
Broad Institute
2009
The complexity of proteomic instrumentation for LC-MS/MS introduces many possible sources variability. Data-dependent sampling peptides constitutes a stochastic element at the heart discovery proteomics. Although this variation impacts identification peptides, identifications are far from completely random. In study, we analyzed interlaboratory data sets NCI Clinical Proteomic Technology Assessment Cancer to examine repeatability and reproducibility in peptide protein identifications....
Cultured tobacco (Nicotiana tabacum var Wisconsin 38) cells adapted to grow under osmotic stress synthesize and accumulate a 26 kilodalton protein (osmotin) which can constitute as much 12% of total cellular protein. In NaCl, osmotin occurs in two forms: an aqueous soluble form (osmotin-I) detergent (osmotin II) the approximate ratio 2:3. Osmotin-I has been purified electrophoretic homogeneity, osmotin-II 90% homogeneity. The N-terminal amino acid sequences osmotins I II are identical...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCapillary electrophoretic separations of proteins using nonionic surfactant coatingsJohn K. Towns and Fred E. RegnierCite this: Anal. Chem. 1991, 63, 11, 1126–1132Publication Date (Print):June 1, 1991Publication History Published online1 May 2002Published inissue 1 June 1991https://pubs.acs.org/doi/10.1021/ac00011a013https://doi.org/10.1021/ac00011a013research-articleACS PublicationsRequest reuse permissionsArticle...
Glycerolpropylsilane bonded phases have been found to control the adsorption and/or denaturation of proteins and nucleic acids on controlled porosity glass supports. The bonded-phase thickness is 18–19Å while amount glycerol moiety varies from 80 150µmoles/g depending support pore diameter. It has demonstrated that carbohydrate supports may be used in chromatography proteins, acids, polysaccharides.
There is an increasing need in biology and clinical medicine to robustly reliably measure tens hundreds of peptides proteins biological samples with high sensitivity, specificity, reproducibility, repeatability. Previously, we demonstrated that LC-MRM-MS isotope dilution has suitable performance for quantitative measurements small numbers relatively abundant human plasma the resulting assays can be transferred across laboratories while maintaining reproducibility precision. Here,...
This paper shows that in situ micromachining can be used to simultaneously position and define (i) support particles, (ii) convective transport channels, (iii) an inlet distribution network of (iv) outlet channels multiple chromatography columns on a single quartz wafer the level few tenths micrometer. Stationary phases were bonded 5 x 10 microns collocated monolith structures separated by rectangular 1.5 wide deep with low degree deviation channel width between top bottom channels. High...
This paper focuses on identifying structural features responsible for resolution of heavy isotope coded peptides during reversed-phase chromatography. was achieved by using labeled coding agents that varied in structure, number deuterium atoms, placement the agent, and functional group targeted reagent. Six were examined. Deuterated versions studied included succinic anhydride-2H4, acetic acid 2,5-dioxopyrrolidin-1-yl ester-2H3, propionic ester-2H5, pentanoic ester-2H9,...
The goal of quantitative proteomics is to examine the expression levels all proteins in a biological system and recognize those that change as function some stimulus. Quantification now frequently based on derivatization peptides with isotopically distinguishable labeling agents. This study examines extent which isotopic forms having same amino acid sequence are resolved by reversed-phase chromatography assesses degree resolution these different peptide impact quantification. Three...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImpact of polycation adsorption on efficiency and electroosmotically driven transport in capillary electrophoresisJohn K. Towns Fred E. RegnierCite this: Anal. Chem. 1992, 64, 21, 2473–2478Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November 1992https://pubs.acs.org/doi/10.1021/ac00045a003https://doi.org/10.1021/ac00045a003research-articleACS PublicationsRequest reuse permissionsArticle...