A5101684960- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Burkholderia infections and melioidosis
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Antimicrobial agents and applications
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Cystic Fibrosis Research Advances
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Spine and Intervertebral Disc Pathology
- Medical Imaging and Analysis
- Metabolomics and Mass Spectrometry Studies
- Scoliosis diagnosis and treatment
- Oral microbiology and periodontitis research
- Meat and Animal Product Quality
- Chemical Looping and Thermochemical Processes
- Tensor decomposition and applications
- Microbial Natural Products and Biosynthesis
University of Hawaiʻi at Mānoa
2010-2024
National University of Singapore
2022
Vietnam Academy of Science and Technology
2020
National Yang Ming Chiao Tung University
2019
University of Florida
2015
University of Calgary
1995-2006
Colorado State University
1997-2002
ABSTRACT Triclosan is an antiseptic frequently added to items as diverse soaps, lotions, toothpaste, and many commonly used household fabrics plastics. Although wild-type Pseudomonas aeruginosa expresses the triclosan target enoyl-acyl carrier protein reductase, it resistant due expression of MexAB-OprM efflux system. Exposure a susceptible Δ( mexAB-oprM ) strain selected multidrug-resistant bacteria at high frequencies. These hyperexpressed MexCD-OprJ system mutations in its regulatory...
One of the hallmarks Pseudomonas aeruginosa infection in cystic fibrosis (CF) patients is very-high-cell-density (HCD) replication lung, allowing this bacterium to induce virulence controlled by quorum-sensing systems. However, nutrient sources sustaining HCD chronic are largely unknown. Here, we performed microarray studies P. directly isolated from lungs CF demonstrate its metabolic capability and vivo. In vivo data, confirmed real-time reverse transcription-PCR, indicated that population...
ABSTRACT The Pseudomonas aeruginosa fabI structural gene, encoding enoyl-acyl carrier protein (ACP) reductase, was cloned and sequenced. Nucleotide sequence analysis revealed that is probably the last gene in a transcriptional unit includes an ATP-binding of ABC transporter unknown function. FabI similar size primary to other bacterial enoyl-ACP reductases, it contained signature motifs for FAD-dependent pyridine nucleotide reductase glucose/ribitol dehydrogenase families, respectively....
Total transcript amplification (TTA) from single eukaryotic cells for transcriptome analysis is established, but TTA a prokaryotic cell presents additional challenges with much less starting material, the lack of poly(A)-tails, and fact that messages can be polycistronic. Here, we describe novel method single-bacterium using model organism, Burkholderia thailandensis , exposed to subinhibitory concentration antibacterial agent, glyphosate. Utilizing B. microarray assess showed low...
ABSTRACT Allelic replacement in the Burkholderia genus has been problematic due to lack of appropriate counter-selectable and selectable markers. The marker sacB , commonly used gram-negative bacteria, is nonselective on sucrose many species. In addition, use antibiotic resistance markers clinical importance for selection desirable genetic traits prohibited United States two potential bioterrorism agents, mallei pseudomallei . Here, we engineered a mutated based B. PheS (the α-subunit...
β-Oxidative enzymes for fatty acid degradation (Fad) of long-chain acids (LCFAs) are induced in vivo during lung infection cystic fibrosis patients, and this may contribute to nutrient acquisition pathogenesis Pseudomonas aeruginosa. The promoter region one P. aeruginosa β-oxidation operon, fadBA5 (PA3014 PA3013), was mapped. Focusing on the transposon mutagenesis strain PAO1 carrying P fadBA5–lacZ fusion, a regulator operon identified be PsrA (PA3006). Transcriptome analysis ΔpsrA mutant...
A close interconnection between nutrient metabolism and virulence factor expression contributes to the pathophysiology of Pseudomonas aeruginosa as a successful pathogen. P. fatty acid (FA) degradation is complicated with multiple acyl-CoA synthetase homologs (FadDs) expressed in vivo lung tissue during cystic fibrosis infections. The promoters two genetically linked fadD genes (fadD1 fadD2) were mapped northern blot analysis indicated they could exist on different transcripts. These FadDs...
The two acyl-homoserine lactones (AHLs) N-(butyryl)-L-homoserine lactone and N-[3-oxododecanoyl]-L-homoserine (3-oxo-C12-HSL) are required for quorum sensing in Pseudomonas aeruginosa. These AHLs derive their invariant rings from S-adenosylmethionine variable acyl chains the cellular acyl-acyl carrier protein (ACP) pool. This reaction is catalysed by specific AHL synthases, which exhibit chain specificity. Culture supernatants of P. aeruginosa contain multiple 3-oxo-AHLs that differ lengths...
The Pseudomonas aeruginosa fabA and fabB genes, encoding beta-hydroxyacyl-acyl carrier protein dehydratase beta-ketoacyl-acyl synthase I, respectively, were cloned, sequenced, expressed in Escherichia coli. Northern analysis demonstrated that are cotranscribed most probably form a fabAB operon. FabA FabB proteins similar size amino acid composition to their counterparts from coli the putative homologs Haemophilus influenzae. Chromosomal mutants isolated; auxotrophic for unsaturated fatty...
Calcium- and integrin-binding protein (CIB) is a novel member of the helix−loop−helix family regulatory calcium-binding proteins which likely has specific function in hemostasis through its interaction with platelet integrin αIIbβ3. The significant amino acid sequence homology between CIB other such as calmodulin, calcineurin B, recoverin suggests that may undergo calcium-induced conformational change; however, mechanism calcium binding details structural change have not yet been...
Pseudomonas aeruginosa can grow to very high-cell-density (HCD) during infection of the cystic fibrosis (CF) lung. Phosphatidylcholine (PC), major component lung surfactant, has been hypothesized support HCD growth P. in vivo. The phosphorylcholine headgroup, a glycerol molecule, and two long-chain fatty acids (FAs) are released by enzymatic cleavage PC bacterial phospholipase C lipases. Three different pathways, choline, glycerol, acid degradation then involved these components. Here, we...
Bacterial cooperative associations and dynamics in biofilm microenvironments are of special interest recent years. Knowledge localized gene-expression corresponding bacterial behaviors within the architecture at a global scale has been limited, due to lack robust technology study limited number cells stratified layers biofilms. With our pioneering developments single cell transcriptomic analysis technology, we generated herein an unprecedented spatial transcriptome map mature vitro...
The Pseudomonas aeruginosa PsrA autorepressor has dual roles as a repressor of the fadBA5beta-oxidation operon and an activator stationary-phase sigma factor rpoS exsCEBA type III secretion system (TTSS). Previously, we demonstrated that repression fadBA5 by is relieved long-chain fatty acids (LCFAs). However, signal affecting activation exsC via unknown. In this study, microarray gene fusion data suggested LCFA (e.g. oleate) affected expression exsC. DNA binding studies confirmed binds to...
ABSTRACT Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and thailandensis were constructed. These combined the effectiveness mini-Tn 7 site-specific transposition system with proteins optimized for spp., enabling bacterial tracking during cellular infection.
The fatty acid (FA) degradation pathway of Pseudomonas aeruginosa, an opportunistic pathogen, was recently shown to be involved in nutrient acquisition during BALB/c mouse lung infection model. source FA the is believed phosphatidylcholine, major component surfactant. Previous research indicated that P. aeruginosa has more than two acyl-CoA synthetase genes (fadD; PA3299 and PA3300), which are responsible for activation FAs using ATP coenzyme A. Through a bioinformatics approach, 11...
Burkholderia pseudomallei, the cause of serious and life-threatening diseases in humans, is national biodefense concern because its potential use as a bioterrorism agent. This microbe listed select agent by CDC; therefore, development vaccines significant importance. Here, we further investigated growth characteristics recently created B. pseudomallei 1026b Δasd mutant vitro, cell model, an animal model infection. The was typified inability to grow absence exogenous diaminopimelate (DAP);...
ABSTRACT A DNA fragment containing the Pseudomonas aeruginosa fabD (encoding malonyl-coenzyme [CoA]:acyl carrier protein [ACP] transacylase), fabG β-ketoacyl-ACP reductase), acpP ACP), and fabF synthase II) genes was cloned sequenced. This fab gene cluster is delimited by plsX a poorly understood enzyme of phospholipid metabolism) pabC 4-amino-4-deoxychorismate lyase) genes; seem to be translationally coupled. The fabH III), which in most gram-negative bacteria located between , absent from...
ABSTRACT Genetic manipulation of the category B select agents Burkholderia pseudomallei and mallei has been stifled due to lack compliant selectable markers. Hence, there is a need for additional select-agent-compliant We engineered marker based on gat gene (encoding glyphosate acetyltransferase), which confers resistance common herbicide (GS). To show ability GS inhibit bacterial growth, we determined effective concentrations against Escherichia coli several species. Plasmids , flanked by...