- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Polymer Surface Interaction Studies
- Silk-based biomaterials and applications
- Catalysis for Biomass Conversion
- Corrosion Behavior and Inhibition
- Viral Infectious Diseases and Gene Expression in Insects
- Electrospun Nanofibers in Biomedical Applications
- Fungal and yeast genetics research
- Surgical Sutures and Adhesives
- Advanced Cellulose Research Studies
- Enzyme-mediated dye degradation
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Fungal Biology and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Wound Healing and Treatments
- Concrete Corrosion and Durability
Norwegian University of Life Sciences
2023
National Renewable Energy Laboratory
2020-2022
Institute of Advanced Study in Science and Technology
2014-2022
Hindustan Petroleum Corporation Limited (India)
2022
Tribhuvan University
2020
Abstract Glycoside Hydrolase Family 7 cellobiohydrolases (GH7 CBHs) catalyze cellulose depolymerization in cellulolytic eukaryotes, making them key discovery and engineering targets. However, there remains a lack of robust structure–activity relationships for these industrially important cellulases. Here, we compare CBHs from Trichoderma reesei ( Tr Cel7A) Penicillium funiculosum Pf Cel7A), which exhibit multi-modular architecture consisting catalytic domain (CD), carbohydrate-binding...
Synthetic multifunctional enzymes exhibit superior performance to their non-multifunctional enzyme components and novel cellulose deconstruction mechanisms.
The corrosion inhibition properties of methanol extract Jatropha curcas (JC) were investigated for mild steel (MS) in acidic medium using weight loss and potentiodynamic methods. Corrosion MS 1MH2SO4 was studied the absence presence various concentrations methanolic barks. results showed an increase with increasing concentration extract. efficiency 1000 ppm JC from polarization methods approximately 92.0% after 24 hours immersion solution. Open circuit potential (OCP) measurements that acts...
Zymomonas mobilis has recently been shown to be capable of producing the valuable platform biochemical, 2,3-butanediol (2,3-BDO). Despite this capability, production high titers 2,3-BDO is restricted by several physiological parameters. One such bottleneck involves conversion acetoin 2,3-BDO, a step catalyzed dehydrogenase (Bdh). Several Bdh enzymes have successfully expressed in Z. mobilis, although highly active enzyme yet identified for expression host. Here, we report application...
Superior lignocellulolytic microbes are useful for efficient bioconversion of plant biomass. Eight fungal strains were isolated from compost and forest litter an Indo-Burma Biodiversity hotspot evaluated their lignolytic cellulolytic capabilities. X-ray diffraction (XRD) study was performed to test effect on cellulose crystallinity rice straw. Scanning electron spectroscopic (SEM) Fourier transform-infrared (FT-IR) analysis the surface morphology chemical changes occurred in fungi treated...
Abstract Heterologous protein production has been challenging in the hyper-cellulolytic fungus, Trichoderma reesei as species is known for poor transformation efficiency, low homologous recombination frequency, and marginal screening systems identification of successful transformants. We have applied 2A-peptide multi-gene expression system to co-express four proteins, which include three cellulases: a cellobiohydrolase (CBH1), an endoglucanase (EG1), β-D-glucosidase (BGL1), well enhanced...