A5042569302- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and disorders
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Fatty Acid Research and Health
- Cardiovascular Health and Risk Factors
- Diet and metabolism studies
- Atherosclerosis and Cardiovascular Diseases
- Health Systems, Economic Evaluations, Quality of Life
- Cardiovascular Function and Risk Factors
- Peroxisome Proliferator-Activated Receptors
- Drug Transport and Resistance Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Liver Diseases and Immunity
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Edible Oils Quality and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Heart Failure Treatment and Management
- Antioxidant Activity and Oxidative Stress
National Institutes of Health Clinical Center
2015-2025
National Institutes of Health
2015-2024
National Heart Lung and Blood Institute
2015-2024
Mayo Clinic in Arizona
2022
ORCID
2021
Bennett Aerospace (United States)
2020
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2018
Sampson County Schools
2013-2017
University of Pennsylvania
2016
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016
Low-density lipoprotein cholesterol (LDL-C), a key cardiovascular disease marker, is often estimated by the Friedewald or Martin equation, but calculating LDL-C less accurate in patients with low level hypertriglyceridemia (triglyceride [TG] levels ≥400 mg/dL).To design more equation for and/or hypertriglyceridemia.Data on and other lipid measures from 8656 seen at National Institutes of Health Clinical Center between January 1, 1976, June 2, 1999, were analyzed β-quantification reference...
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest global profiling, i.e., semiquantitative monitoring all chemical constituents biological fluids. In this work, we performed profiling NIST SRM 1950, "Metabolites Human Plasma", using GC-MS, LC-MS, NMR. Metabolome coverage, difficulties, reproducibility experiments on each platform are discussed. A total 353 metabolites been identified material. GC-MS provides 65 unique...
Abstract Purpose: To determine whether mesothelin, a cell surface protein highly expressed in mesothelioma and ovarian cancer, is shed into serum if so to accurately measure it. Experimental Design: We developed sandwich ELISA using antibodies reacting with two different epitopes on human mesothelin. quantitate mesothelin levels, standard curve was generated mesothelin-Fc fusion protein. Sera from 24 healthy volunteers, 95 random hospital patients, 56 patients mesothelioma, 21 cancer were...
Hepatitis C virus (HCV) modulates intrahepatic cholesterol biosynthetic pathways to promote viral replication. Chronic HCV infection is associated with altered metabolism, including dyslipidemia and insulin resistance (IR), which contributes disease progression influences response therapy. To further understand the impact of on host we examined changes in serum lipid profiles expression lipid-related genes during interferon (IFN)-free treatment chronic HCV, genotype 1 sofosbuvir ribavirin...
Increased small dense low-density lipoprotein-cholesterol (sdLDL-C) is a risk factor for atherosclerotic cardiovascular disease (ASCVD) but typically requires advanced lipid testing. We describe two new equations, first one calculating large buoyant LDL-C (lbLDL-C), based only upon results from the standard panel, and second sdLDL-C.Equations sdLDL-C lbLDL-C were generated with least-squares regression analysis using direct Denka assay as reference (n = 20 171). was assessed risk-enhancer...
We determined whether the physiologic changes that accompany food intake or sympathetic activation by β-adrenergic stimulation result in alterations secretion of leptin, tumor necrosis factor-α (TNFα), interleukin-6 (IL-6) serially sampling sc abdominal adipose interstitial fluid open-flow microperfusion before and after a standardized meal response to isoproterenol (1 μmol/L) delivered locally. Post cibum IL-6 rose up 5-fold, whereas leptin TNFα did not change; TNFα, but IL-6, correlated...
We hypothesized that patients with high HDL-cholesterol (HDL-C) and ischemic heart disease (IHD) may have dysfunctional HDL or unrecognized nonconventional risk factors.Individuals IHD (Copenhagen University Hospital) either HDL-C (n = 53; women >or=735 mg/L; men >or=619 mg/L) low 42; <or=387 <or=341 were compared individuals without City Heart Study) matched by age, sex, concentrations 110). All participants had within reference intervals for LDL-C (<1600 triglyceride (<1500 mg/L), none...
Although lipids, apolipoproteins, and lipoprotein particles are important modulators of inflammation, varying relationships exist between these parameters asthma.To determine whether serum lipids apolipoproteins correlate with the severity airflow obstruction in subjects atopy asthma.Serum samples were obtained from 154 atopic nonatopic without asthma, 159 asthma. Serum lipid levels quantified using standard diagnostic assays nuclear magnetic resonance (NMR) spectroscopy. Airflow was...
Low high-density lipoprotein-cholesterol (HDL-C) in patients with coronary heart disease (CHD) may be caused by rate-limiting amounts of lecithin:cholesterol acyltransferase (LCAT). Raising LCAT beneficial for CHD, as well familial deficiency, a rare disorder low HDL-C.To determine safety and tolerability recombinant human infusion subjects stable CHD HDL-C its effect on plasma lipoproteins.A phase 1b, open-label, single-dose escalation study was conducted to evaluate safety, tolerability,...
Elevated low-density lipoprotein cholesterol (LDL-C) is an important risk factor in the development of atherosclerotic cardiovascular disease (ASCVD). Inhibitors proprotein convertase subtilisin/kexin type 9 (PCSK9), a negative regulator LDL-C metabolism, have emerged as promising approaches for reducing elevated levels. Here, we evaluated cholesterol-lowering efficacy virus-like particle (VLP) based vaccines that target epitopes found within LDL receptor (LDL-R) binding domain PCSK9. In...
Statins lower plasma cholesterol by as much 50%, thus reducing future cardiovascular events. However, the physiological effects of statins are diverse and not all related to low density lipoprotein (LDL-C) lowering. We performed a small clinical pilot study assess impact on lipoprotein-associated proteins in healthy individuals (n = 10) with normal LDL-C (<130 mg/dL), who were treated rosuvastatin (20 mg/day) for 28 days. Proteomic analysis size-exclusion chromatography isolated LDL, large...
Lipoprotein particle measures performed by nuclear magnetic resonance (NMR), and associated ratios, may be better markers for atherosclerosis risk than conventional lipid measures. The effect of two functional olive oils, one enriched with its polyphenols (FVOO, 500 ppm), the other (FVOOT) them (250 ppm) those thyme versus a standard virgin oil (VOO), on lipoprotein atherogenic ratios subclasses profiles was assessed.In randomized, double-blind, crossover, controlled trial, 33...
Apolipoprotein C-II (apoC-II) is a cofactor for lipoprotein lipase, plasma enzyme that hydrolyzes triglycerides (TGs). ApoC-II deficiency in humans results hypertriglyceridemia. We used zinc finger nucleases to create <i>Apoc2</i> mutant mice investigate the use of C-II-a, short apoC-II mimetic peptide, as therapy deficiency. Mutant produced form with an uncleaved signal peptide preferentially binds high-density lipoproteins (HDLs) due 3-amino acid deletion at cleavage site. Homozygous had...
Scope Palmitoleic acid (palmitoleate; C16:1 n‐7), an omega‐7 monounsaturated fatty (MUFA) found in plants and marine sources, has been shown to favorably modulate lipid glucose metabolism. However, its impact on atherosclerosis not examined detail. Methods Results LDL receptor knock out (LDLR‐KO) mice are fed a Western diet supplemented with 5% (w/w) palmitoleate concentrate, oleic‐rich olive oil, or none (control) for 12 weeks. Dietary increases hepatic levels, improves plasma...
A complex interplay exists between plasma lipoproteins and inflammation, as evidenced from studies on atherosclerosis. Alterations in lipoprotein levels the context of infectious diseases, particularly respiratory viral infections, such SARS-CoV-2, have become great interest recent years, due to their potential utility prognostic markers. Patients with severe COVID-19 been reported low total cholesterol, HDL-cholesterol, LDL-cholesterol, but elevated triglycerides. However, a detailed...
Abstract Background The accurate measurement of Low-density lipoprotein cholesterol (LDL-C) is critical in the decision to utilize new lipid-lowering therapies like PCSK9-inhibitors (PCSK9i) for high-risk cardiovascular disease patients that do not achieve sufficiently low LDL-C on statin therapy. Objective To improve estimation by developing a equation includes apolipoprotein B (apoB) as an independent variable, along with standard lipid panel test results. Methods Using β-quantification (...
Anthocyanin-rich products (RFA and AI) decreased LDLc; ApoB; total, large, small LDL-P; LDL size; TG/HDL ratio, large TRL, GlycA alanine versus WFA. Thus, anthocyanin-rich provided greater protection against CVD risk than
This single center, double-blinded, cross-over, placebo controlled clinical trial investigated the effect of oral α-cyclodextrin (α-CD), a soluble dietary fiber, on blood lipid and lipoprotein levels in healthy human subjects. α-CD, cyclical polymer containing 6 glucose subunits, is currently sold as an over counter food supplement also common additive many foods. α-CD forms hydrophobic central cavity that binds lipids has been shown animal studies previous trials to alter plasma levels. We...
Background: Eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) have both shared different cardiovascular effects, commonly used fish oil supplements considerably varied EPA/DHA ratios. Aims: We compared the effects of with ratios on lipoprotein metabolism. Methods: In a double-blind, randomized cross-over study, normolipidemic adults (n = 30) consumed 12 g/day EPA-rich (EPA/DHA: 2.3) or DHA-rich 0.3) for 8-weeks, separated by an 8-week washout period. Results: Both similarly lowered...
Abstract Background Standard lipid panel assays employing chemical/enzymatic methods measure total cholesterol (TC), triglycerides (TG), and high-density lipoprotein (HDL-C), from which are calculated estimates of low-density (LDL-C). These measures used universally to guide management atherosclerotic cardiovascular disease risk. Apolipoprotein B (apoB) is generally acknowledged be superior LDL-C for lipid-lowering therapeutic decision-making, but apoB immunoassays performed relatively...