The physiological functions of lysophosphatidylethanolamine (lysoPE) have not been fully elucidated. In this study, the effects lysoPE on lipogenesis and lipolysis were investigated in a cultured human liver-derived cell line. intracellular lipid profile was detail using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to better understand underlying mechanism. expression genes related metabolism catabolism analyzed real-time PCR. LysoPE supplementation induced cellular droplet...

Rabbit morulae were exposed to a vitrification solution-modified PBS [PB1] medium containing 40% ethylene glycol + 18% Ficoll 0.3 M sucrose (EFS) for 2, 5, or 10 min at 20 ° C and vitrified in liquid nitrogen. When rapidly warmed, 96% had an intact zona pellucida. embryos cultured after removal of the mucin coat, high proportions them formed blastocoel (79−100%), but percentage developed fully expanded blastocysts decreased with increased exposure time 87%, 40%, 17%). The survival rate coat...

Vapor- and gas-responsive ionic liquids (ILs) comprised of cationic metal-chelate complexes bis(trifluoromethanesulfonyl)imide (Tf(2)N) have been prepared, namely, [Cu(acac)(BuMe(3)en)][Tf(2)N] (1 a), [Cu(Bu-acac)(BuMe(3)en)][Tf(2)N] b), [Cu(C(12)-acac)(Me(4)en)][Tf(2)N] c), [Cu(acac)(Me(4)en)][Tf(2)N] d), [Ni(acac)(BuMe(3)en)][Tf(2)N] (2 a) (acac = acetylacetonate, Bu-acac 3-butyl-2,4-pentanedionate, C(12)-acac 3-dodecyl-2,4-pentanedionate, BuMe(3)en...

In the course of experiments to identify and characterize factors that function in bovine conceptuses during peri-attachment periods, various transcripts related epithelial-mesenchymal transition (EMT) were found. this study, RNA was extracted from different sets days 17, 20, 22 (day 0=day estrous) subjected real-time PCR analysis as well Western blotting, which abundances N-cadherin (CDH2), vimentin, matrix metalloproteinase 2 (gelatinase A, 72 kDa gelatinase, type IV collagenase) (MMP2),...

RNA-binding proteins provide a new layer of posttranscriptional regulation RNA during cancer progression. We identified motif protein 47 (RBM47) as target gene transforming growth factor (TGF)-β in mammary gland epithelial cells (NMuMG cells) that have undergone the epithelial-to-mesenchymal transition. TGF-β repressed RBM47 expression NMuMG and lung cell lines. Expression correlated with good prognosis patients lung, breast gastric cancer. suppressed metabolism-related genes, which were...

The optimal dietary fat content to induce clinical remission in active Crohn's disease has been the subject of controversy. We therefore performed a prospective, randomized, controlled study compare effects nutrient formulas differing amount medium-chain triglycerides (MCT).Thirty-six patients with whose activity index (CDAI) was > or =150 were included study. A formula 3.4 g per 2000-kcal dose used as low-fat (ED group), and 55.6 high MCT (TL group).The rate short-term induction at 6 weeks...

A high number of lZ6I-activin-A binding sites (an apparent Kd 260 p~ and 6,600 sites/cell) were observed on MC3T3-El cells, a well characterized osteoblastic cell line.Activin-A has mitogenic effect these with the greatest influence being cells in an undifferentiated state, as suppressive alkaline phosphatase activity.Northern ligand blotting analyses revealed that produce follistatin, which was down-regulated by retinoic acid treatment.Because follistatin is activin-A-binding protein, we...

Using an oxygen radical absorbance capacity (ORAC) assay, antioxidant activity was detected in the ethanol extract of Pacific oyster, which purified by sequential extraction with organic solvents. The ethyl acetate fraction showed strongest and further purified, yielding a single compound [as assessed thin-layer chromatography (TLC) reverse-phase high-performance liquid (HPLC)]. This identified as 3,5-dihydroxy-4-methoxybenzyl alcohol on basis 1H 13C nuclear magnetic resonance (NMR),...

Apolipoprotein C-II (apoC-II) is a cofactor for lipoprotein lipase, plasma enzyme that hydrolyzes triglycerides (TGs). ApoC-II deficiency in humans results hypertriglyceridemia. We used zinc finger nucleases to create <i>Apoc2</i> mutant mice investigate the use of C-II-a, short apoC-II mimetic peptide, as therapy deficiency. Mutant produced form with an uncleaved signal peptide preferentially binds high-density lipoproteins (HDLs) due 3-amino acid deletion at cleavage site. Homozygous had...

Abundant secretory immunoglobulin A (SIgA) in the mucus, breast milk, and saliva provides immunity against infection of mucosal surfaces. Pre-pandemic milk samples containing SIgA have been reported to cross-react with SARS-CoV-2; however, it remains unknown whether showing cross-reaction SARS-CoV-2 exists saliva. We aimed clarify cross-reacts spike 1 subunit individuals who not infected this virus. The study involved 137 (men, n = 101; women, 36; mean age, 38.7; age range, 24-65 years)...

Cytomegalovirus infection has been known to complicate ulcerative colitis. This study was designed elucidate the prevalence and clinical features of colitis that might point efficiently diagnosis complicating cytomegalovirus in cases colitis.The included 47 consecutive patients diagnosed have moderate-to-severe who were treated on an inpatient basis at our department during a two-year period. A prospective examination for antigenemia conducted all with determine among these patients. Then,...

Three cases sharing the following radiologic features are reported: (a) abdominal conventional radiography—vascular calcifications at right hemicolon, (b) computed tomography—colonic wall thickening and venous calcifications, (c) barium enema examination—luminal narrowing of hemicolon thumbprinting. There were no clinical or laboratory findings suggestive portal hypertension. The disease entity, "phlebosclerotic colitis," should be differentiated from ordinary ischemic colitis.

Background and Study Aims: Although balloon dilation is widely used in nonmalignant pyloric stenosis, little information available on either the short-term or long-term results of this type therapy patients with obstructive gastroduodenal Crohn's disease.

Interferon tau gene (IFNT) is expressed only by mononuclear trophectoderm cells in ruminant ungulates. To our knowledge, its epigenetic regulation and interaction with lineage-specific caudal-related homeobox 2 transcription factor (CDX2) have not been characterized. Herein, we studied differences chromatin structures of endogenous bovine IFNT trophoblast BT-1 CT-1 nontrophoblast MDBK cells. Transcripts from CDX2 genes were found but Chromatin immunoprecipitation study revealed that binding...

Expression of interferon-tau (IFNT), necessary for pregnancy establishment in ruminant ungulates, is regulated a temporal and spatial manner. However, molecular mechanisms by which IFNT gene transcription this manner have not been firmly established. In study, DNA microarray/RT-PCR analysis between bovine trophoblast CT-1 Mardin-Darby kidney (MDBK) cells was initially performed, finding that factors GATA2, GATA3, GATA6 mRNAs were specific to cells. These also found Days 17, 20, 22 (Day 0 =...

Apolipoprotein A-I (apoA-I) mimetic peptides are currently being developed as possible new agents for the treatment of cardiovascular disease based on their ability to promote cholesterol efflux and other beneficial antiatherogenic properties. Many these peptides, however, have been reported cause transient hypertriglyceridemia due inhibition lipolysis by lipoprotein lipase (LPL). We describe a novel bihelical amphipathic peptide (C-II-a) that contains an helix (18A) binding lipoproteins...

Alkyloctamethylferrocenium salts with the Tf2N anion ([Fe(C5Me4C(n)H(2n+1))(C5Me4H)][Tf2N]; = bis(trifluoromethanesulfonyl)amide) were prepared, and their ionic liquid properties, thermal crystal structures, magnetic properties investigated. The melting points of near room temperature, decreased increasing alkyl chain length up to n 8 then increased. PF6 NO3 anions also prepared. higher than 100 °C. Most these exhibited phase transitions in solid state. sum entropies was nearly independent...