A5077933669- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Gastric Cancer Management and Outcomes
- Marine and coastal ecosystems
- Arsenic contamination and mitigation
- Lipid Membrane Structure and Behavior
- Environmental Toxicology and Ecotoxicology
- Cellular transport and secretion
- Microbial Community Ecology and Physiology
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Food composition and properties
- Biocrusts and Microbial Ecology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Proteoglycans and glycosaminoglycans research
- Metastasis and carcinoma case studies
- Enzyme function and inhibition
- Microbial Metabolites in Food Biotechnology
- Paleontology and Stratigraphy of Fossils
- Ion channel regulation and function
- Gastrointestinal Tumor Research and Treatment
- Marine and coastal plant biology
- Marine Sponges and Natural Products
- Coral and Marine Ecosystems Studies
Tokyo University of Pharmacy and Life Sciences
2015-2024
The University of Tokyo
1990-2023
Kagawa University
2020-2022
Kihoku hospital
2018
Wakayama Medical University
2018
Fukuoka University
2012-2018
American Society for Gastrointestinal Endoscopy
2013
Tokyo University of Agriculture and Technology
2013
Japan Science and Technology Agency
2005-2012
Kobe University
1992-2011
Abstract We have established a high-efficiency method for transforming the unicellular, green alga Chlamydomonas reinhardtii by electroporation. Electroporation of strains CC3395 and CC425, cell wall-less mutants devoid argininosuccinate lyase (encoded ARG7), in presence plasmid pJD67 (which contains ARG7) was used to optimize conditions introduction exogenous DNA. The that were varied included osmolarity, temperature, concentration DNA, voltage capacitance. Following optimization, maximum...
cDNA clones for the periplasmic carbonic anhydrase (CA; carbonate hydro-lyase, EC 4.2.1.1) of Chlamydomonas reinhardtii cells were isolated and characterized. The fact that cloned fragments encoded a 377-amino acid polypeptide (41,626 Da) consisting an NH2-terminal hydrophobic signal peptide 20 amino acids, large (35,603 subunit polypeptide, small (4144 indicates two subunits are cotranslated as precursor polypeptide. sequence mature deduced from nucleotide showed 20-22% homology with human...
Two copies of structurally related genes (CAH1 and CAH2) for carbonic anhydrase (EC 4.2.1.1) were found to be tandemly clustered on the Chlamydomonas reinhardtii genome. The previously isolated cDNA clones polypeptides derived from upstream gene, CAH1, which has 10 introns in its coding region. downstream CAH2, also introns, at positions identical those CAH1. Although amino acid sequences deduced two showed 91.8% identity, partial authentic enzyme air-induced cells only CAH1 product....
ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of synthesis in cyanobacteria. Mutants defective each these enzymes Synechococcus elongatus PCC 7942 were constructed characterized. Activities corresponding selected mutants virtually undetectable, their ability to synthesize was entirely abolished. The maximal activities photosynthetic O(2) evolution rates respiration dark significantly decreased compared those wild-type cells. Addition 0.2 M...
Abstract Membrane lipid remodeling contributes to the environmental acclimation of plants. In green lineage, a betaine lipid, diacylglyceryl- N , -trimethylhomoserine (DGTS), is included exclusively among algae and nonflowering Here, we show that alga Chlorella kessleri synthesizes DGTS under phosphorus-deficient conditions through eukaryotic pathway via ER. Simultaneously, phosphatidylcholine phosphatidylethanolamine, which are similar in their zwitterionic properties, almost completely...
It is widely accepted that green plants evolved the capacity to synthesize highly organized branched alpha-polyglucan amylopectin with tandem-cluster structure, whereas animals and bacteria continued produce random glycogen. Although most previous studies documented cyanobacteria accumulate glycogen, present study shows explicitly some such as Cyanobacterium sp. MBIC10216, Myxosarcina burmensis Synechococcus BG043511 had distinct alpha-polyglucans, which were designated semi-amylopectin. The...
Abstract Background and aims Intestinal metaplasia (IM) of the stomach is associated with an increased risk differentiated gastric cancer. While it important to diagnose IM endoscopically, can be difficult observe by white-light endoscopy. In magnifying endoscopy narrow-band imaging (M-NBI) stomach, a light-blue crest (LBC) widely known useful marker in endoscopic diagnosis IM. However, that exhibits only white opaque substance (WOS) without LBC also occur. The aim this study was elucidate...
The plant pathogenic bacterium Ralstonia solanacearum injects more than 70 effector proteins (virulence factors) into the host cells via needle-like structure of a type III secretion system. system manipulate regulatory networks to suppress defense responses with diverse molecular activities. Uncovering function these effectors is essential for mechanistic understanding R. pathogenicity. However, few from have been functionally characterized, and their targets remain largely unknown. Here,...
Plastoquinone plays a crucial role in the photosynthetic electron transport system as an carrier, transferring electrons from photosystem II to cytochrome b 6 f complexes. Certain cyanobacteria acylate plastoquinone derivatives, plastoquinol, reduced form of plastoquinone, and/or plastoquinone-C, hydroxylated synthesize newly found cyanobacterial lipids, acylplastoquinol and acylplastoquinone-C, latter which is known plastoquinone-B seed plants. The genes, slr2103 Synechocystis sp. PCC 6803...
We examined the short-term metabolic processes of arsenate for 24 h in a freshwater unicellular green alga, Chlamydomonas reinhardtii wild-type strain CC-125. The arsenic species algal extracts were identified by high-performance liquid chromatography/inductively coupled plasma mass spectrometry after water extraction using sonicator. Speciation analyses showed that levels arsenite, arsenate, and methylarsonic acid cells rapidly increased 30 min to 1 h, those dimethylarsinic...
Storage glucans were analyzed in the Porphyridiales which include most primitive and phylogenetically diverged species Rhodophyta, to understand early evolution of glucan structure Rhodophyta. The storage both Galdieria sulphuraria Cyanidium caldarium consisted glycogen, while those Rhodosorus marinus, Porphyridium purpureum, P. sordidum Rhodella violacea could be defined as semi-amylopectin. X-ray diffraction analysis demonstrated variation crystalline structure: patterns purpureum R. A-...
A phylogeny of 21 haptophyte algae was inferred by maximum parsimony, neighbor‐joining, and likelihood analyses sequences the plastid‐encoded gene, rbc L. Sequence variation in spacer region RUBISCO operon also investigated. In all L trees constructed, haptophytes form two distinct clades: one includes Pavlovales other Prymnesiales, Coccosphaerales, Isochrysidales (all sensu Parke Green 1976 . This relationship coincides with recent taxonomic treatment splitting division into subclasses,...
Although patients with gynecological malignancies now survive longer due to advances in early diagnosis and therapy, major issues still remain regarding the quality of life for survivors. Surgical menopause increases risk atherosclerosis; however, few studies have investigated influence platinum-based adjuvant chemotherapy. This study was conducted evaluate effects chemotherapy on atherosclerosis.This enrolled 47 women (26 ovarian cancers 21 endometrial cancers) who underwent surgical...
Environmental contextAlthough arsenic is known to accumulate in both marine and freshwater ecosystems, the pathways by which accumulated transferred systems are reasonably unknown. This study revealed that cyanobacteria have ability produce arsenosugars from inorganic compounds. The findings suggest not only algae, but cyanobacteria, play an important role cycle of aquatic ecosystems. AbstractMetabolic processes incorporated arsenate axenic cultures Synechocystis sp. PCC 6803 Nostoc...