A5083590484- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Bacterial Genetics and Biotechnology
- Plant tissue culture and regeneration
- Environmental Chemistry and Analysis
- Trace Elements in Health
- Chromium effects and bioremediation
- Banana Cultivation and Research
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Analytical chemistry methods development
- Pharmaceutical and Antibiotic Environmental Impacts
- Toxic Organic Pollutants Impact
- Bacteriophages and microbial interactions
- Corrosion Behavior and Inhibition
- Plant Surface Properties and Treatments
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Iron Metabolism and Disorders
Center for Research in Agricultural Genomics
2016-2025
Universitat de Barcelona
2016-2025
Laboratoire des Interactions Plantes Micro-Organismes
2006-2022
Université de Toulouse
2021
Southeast University
2021
Centre National de la Recherche Scientifique
2006-2021
Universitat Autònoma de Barcelona
2012-2017
Baylor Genetics
2017
LMU Klinikum
2014
Klinikum rechts der Isar
2014
In many plant and animal bacterial pathogens, the Type III secretion system (TTSS) that directly translocates effector proteins into eukaryotic host cells is essential for development of disease. all species studied, transcription TTSS most its substrates tightly regulated by a succession consecutively activated regulators. However, whole genetic programme driven these regulatory cascades still unknown, especially in pathogens. Here, we have characterised triggered HrpG, host-responsive...
Solanum commersonii is a wild potato species that exhibits high tolerance to both biotic and abiotic stresses has been used as source of genes for introgression into cultivated potato. Among the interesting features S. resistance bacterial wilt caused by Ralstonia solanacearum, one most devastating diseases crops. In this study, we deep sequencing RNA (RNA-seq) analyze below-ground plant transcriptional responses R. solanacearum. While majority RNA-seq reads could be aligned tuberosum Group...
Xylem vascular wilt pathogens cause devastating diseases in plants. Proliferation of these the xylem causes massive disruption water and mineral transport, resulting severe wilting death infected Upon reaching tissue, multiply profusely, spreading vertically within sap, horizontally between vessels to surrounding tissues. Plant resistance is very complex. One most effective defense responses resistant plants formation physico-chemical barriers tissue. Vertical spread vessel lumen restricted...
Mitogen-activated protein kinase (MAPK) cascades have important functions in plant stress responses and development are key players reactive oxygen species (ROS) signalling innate immunity. In Arabidopsis, the transmission of ROS pathogen by MAPKs involves coordinated activation MPK6 MPK3; however, specificity their negative regulation phosphatases is not fully known. Here, we present genetic analyses showing that MAPK phosphatase 2 (MKP2) regulates oxidative defence functionally interacts...
The ability of Ralstonia solanacearum to cause disease in plants depends on its type III secretion system (T3SS). expression the T3SS and effector substrates is coordinately controlled by a regulatory cascade, at bottom which HrpB. Transcription hrpB gene activated plant-responsive regulator named HrpG, master wide array pathogenicity functions R. solanacearum. We have identified genome strain GMI1000 close paralog hrpG (83% overall similarity protein level) that we prhG. Despite this high...
This article exhaustively reviews current knowledge on the type III as main virulence determinant of R. solanacearum. Recent findings this topic are interpreted in plant‐pathogen research context and future directions proposed.
Although much is known about the signals that trigger transcription of virulence genes in plant pathogens, their prevalence and timing during infection are still unknown. In this work, we address these questions by analysing expression main pathogenicity determinants bacterial pathogen Ralstonia solanacearum. We set up a quantitative, non-invasive luminescent reporter to monitor planta from single promoters chromosome. show new provides real-time measure promoter output vivo - either after...
Ralstonia solanacearum is a bacterial vascular pathogen causing devastating wilt. In the field, resistance against this quantitative and available for breeders only in tomato eggplant. To understand basis of to R. tomato, we investigated spatio-temporal dynamics colonization using non-invasive live monitoring techniques coupled grafting susceptible resistant varieties. We found four 'bottlenecks' that limit bacterium tomato: root colonization, vertical movement from roots shoots, circular...
Twitching and swimming are two bacterial movements governed by pili flagella. The present work identifies for the first time in Gram-negative plant pathogen Ralstonia solanacearum a pilus-mediated chemotaxis pathway analogous to that governing flagellum-mediated chemotaxis. We show regulatory genes this control all of phenotypes related pili, including twitching motility, natural transformation, biofilm formation, also directly implicated virulence, mainly during steps infection. Our results...
Abstract Background Ralstonia solanacearum is the causal agent of bacterial wilt, a devastating plant disease responsible for serious economic losses especially on potato, tomato, and other solanaceous species in temperate countries. In R. , gene expression analysis has been key to unravel many virulence determinants as well their regulatory networks. However, most these assays have performed using either bacteria grown minimal medium or planta after symptom onset, which occurs at late...
Summary Tomato varieties resistant to the bacterial wilt pathogen Ralstonia solanacearum have ability restrict movement in plant. Inducible vascular cell wall reinforcements seem play a key role confining R. into xylem vasculature of tomato. However, type compounds involved such physico‐chemical barriers remain understudied, while being component resistance. Here we use combination histological and live‐imaging techniques, together with spectroscopy gene expression analysis understand nature...
Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute maintaining protein homeostasis. These membraneless organelles dynamic, disassembling once the is no longer present. Persistence of SGs due mutations or chronic has been often related age-dependent protein-misfolding diseases animals. Here, we find metacaspase MC1 dynamically recruited into upon proteotoxic Arabidopsis (Arabidopsis thaliana). Two predicted disordered regions,...
We describe here the construction of a delivery system for stable and directed insertion gene constructs in permissive chromosomal site bacterial wilt pathogen Ralstonia solanacearum. The consists collection suicide vectors-the chromosome (pRC) series-that carry an integration element flanked by transcription terminators two sequences homology to strain GMI1000, where is inserted through double recombination event. Unique restriction enzyme sites GATEWAY cassette enable cloning any...
Ralstonia solanacearum is a soil and water-borne pathogen that can infect wide range of plants cause the devastating bacterial wilt disease. To successfully colonize host, R. requires type III secretion system (T3SS), which delivers effector proteins inside plant cells. HrpG central transcriptional regulator drives expression T3SS other virulence determinants. hrpG transcription highly induced upon cell contact its product also post-transcriptionally activated by metabolic signals present...
We present here the characterization of a new gene family, awr, found in all sequenced Ralstonia solanacearum strains and other bacterial pathogens. demonstrate that five paralogues strain GMI1000 encode type III-secreted effectors deletion awr genes severely impairs its capacity to multiply natural host plants. Complementation studies show AWR (alanine-tryptophan-arginine tryad) display some functional redundancy, although AWR2 is major contributor virulence. In contrast, devoid (Δawr1-5)...
Abstract Bacterial pathogens possess complex type III effector (T3E) repertoires that are translocated inside the host cells to cause disease. However, only a minor proportion of these effectors have been assigned function. Here, we show T3E AWR5 from phytopathogen Ralstonia solanacearum is an inhibitor TOR, central regulator in eukaryotes controls switch between cell growth and stress responses response nutrient availability. Heterologous expression yeast caused inhibition autophagy...
Summary The hypersensitive response ( HR ) is a localized programmed cell death phenomenon that occurs in to pathogen recognition at the site of attempted invasion. Despite more than century research on , little known about how it so tightly regulated and can be contained spatially few cells. At MC 1 an Arabidopsis thaliana plant metacaspase positively regulates . Here, we used unbiased approach identify new regulators. Immunoaffinity purification 1‐containing complexes led us identification...
The plant pathogenic bacterium Ralstonia solanacearum injects more than 70 effector proteins (virulence factors) into the host cells via needle-like structure of a type III secretion system. system manipulate regulatory networks to suppress defense responses with diverse molecular activities. Uncovering function these effectors is essential for mechanistic understanding R. pathogenicity. However, few from have been functionally characterized, and their targets remain largely unknown. Here,...
ABSTRACT Plants utilize cell surface-localized pattern recognition receptors (PRRs) and intracellular nucleotide-binding leucine-rich repeat (NLR) to detect non-self elicit robust immune responses. Fine-tuning the homeostasis of these is critical prevent their hyperactivation. Here, we show that Arabidopsis plants lacking metacaspase 1 ( At MC1) display autoimmunity dependent on signalling components downstream NLR PRR activation. Overexpression a catalytically inactive MC1 in an atmc1...