- Plant Surface Properties and Treatments
- Postharvest Quality and Shelf Life Management
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Potato Plant Research
- Tree Root and Stability Studies
- Plant responses to water stress
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Disease Management Techniques
- Fungal Plant Pathogen Control
- Plant-Microbe Interactions and Immunity
- Horticultural and Viticultural Research
- Educational Technology in Learning
- Epigenetics and DNA Methylation
- Plant Pathogens and Resistance
- HER2/EGFR in Cancer Research
- Plant Pathogenic Bacteria Studies
- Breast Cancer Treatment Studies
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Libraries, Manuscripts, and Books
- Genomics, phytochemicals, and oxidative stress
University of Girona
2014-2024
Italian Oncology Group of Clinical Research
2024
University of Parma
2018-2024
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2024
Institut de Biologie Moléculaire des Plantes
2008
University of Bonn
2008
Centre National de la Recherche Scientifique
2008
Abstract Cork (phellem) is a multilayered dead tissue protecting plant mature stems and roots healing tissues from water loss injuries. cells are made impervious by the deposition of suberin onto cell walls. Although cork formation essential for survival land plants, molecular studies have rarely been conducted on this tissue. Here, we address question combining suppression subtractive hybridization together with cDNA microarrays, using as model external bark tree (Quercus suber), which...
Suberin and waxes embedded in the suberin polymer are key compounds control of transpiration tuber periderm potato (Solanum tuberosum). is a cell-wall biopolymer with aliphatic aromatic domains. The consists fatty acid polyester esterified ferulic acid, which thought to play an important role cross-linking domain. In potato, esters also main components wax. How these ferulate contribute water barrier remains unknown. Here we report on gene encoding ω-hydroxyacid/fatty alcohol...
Germline variants occurring in BRCA1 and BRCA2 give rise to hereditary breast ovarian cancer (HBOC) syndrome, predisposing breast, ovarian, fallopian tube, peritoneal cancers marked by elevated incidences of genomic aberrations that correspond poor prognoses. These genes are fact involved genetic integrity, particularly the process homologous recombination (HR) DNA repair, a high-fidelity repair system for mending double-strand breaks. In addition its implication HBOC pathogenesis,...
Abstract Suberin is a cell wall lipid polyester found in the cork cells of periderm offering protection against dehydration and pathogens. Its biosynthesis assembly, as well its contribution to sealing properties periderm, are still poorly understood. Here, we report on isolation coding sequence CYP86A33 molecular physiological function this gene potato (Solanum tuberosum) tuber periderm. was down-regulated plants by RNA interference-mediated silencing. Periderm from CYP86A33-silenced...
Very long chain aliphatic compounds occur in the suberin polymer and associated wax. Up to now only few genes involved biosynthesis have been identified. This is a report on isolation of potato (Solanum tuberosum) 3-ketoacyl-CoA synthase (KCS) gene study its molecular physiological relevance by means reverse genetic approach. gene, called StKCS6, was stably silenced RNA interference (RNAi) potato. Analysis chemical composition tuber periderms indicated that StKCS6 down-regulation has...
Suberin and wax deposited in the cork (phellem) layer of periderm form lipophilic barrier that protects mature plant organs. Periderm lipids have been widely studied for their protective function with regards to dehydration how they respond environmental stresses wounding. However, despite advances biosynthetic pathways suberin associated wax, little is known about regulation deposition. Here, we report on a potato NAC transcription factor gene, StNAC103, induced tuber phellem (skin). The...
Summary Tomato varieties resistant to the bacterial wilt pathogen Ralstonia solanacearum have ability restrict movement in plant. Inducible vascular cell wall reinforcements seem play a key role confining R. into xylem vasculature of tomato. However, type compounds involved such physico‐chemical barriers remain understudied, while being component resistance. Here we use combination histological and live‐imaging techniques, together with spectroscopy gene expression analysis understand nature...
The present study provides new insights on the role of potato (Solanum tuberosum) suberin feruloyl transferase FHT in native and wound tissues, leading to conclusions about hitherto unknown properties phellogen. In agreement with enzymatic FHT, it is shown that its transcriptional activation protein accumulation are specific tissues undergo suberization such as root boundary layers exodermis endodermis, along tuber periderm. Remarkably, expression within periderm restricted phellogen...
Abstract Cork is a water-impermeable, suberin-based material harboring lignin, (hemi)cellulose, and extractable small molecules (primarily triterpenoids). Extractables strongly influence the properties of materials. Though these previous findings suggest key role for triterpenoids in cork quality, directly testing this idea hindered part because it not known which genes control triterpenoid biosynthesis. Here, we used gas chromatography mass spectrometry to determine that majority (>85%)...
The molecular processes underlying cork biosynthesis and differentiation are mostly unknown. Recently, a list of candidate genes for regulation was made available opening new possibilities studies in oak (Quercus suber L.). Based on this list, we analyzed the seasonal variation mRNA abundance tissue selected by real time reverse-transcriptase polymerase chain reaction (RT-PCR). Relative transcript evaluated principal component analysis were clustered several functional subgroups. Structural...
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding outer skin and subsequent unfavorable healing conditions. Motivated by both economic nutritional considerations, metabolite profiling study aims improve understanding closing layer wound periderm formation guide development new methods ensure faster more complete after breakage. The polar metabolites wound-healing tissues from four potato cultivars with...
Periderms present in plant barks are essential protective barriers to water diffusion, mechanical breakdown, and pathogenic invasion. They consist of densely packed layers dead cells with cell walls that embedded suberin. Understanding the interplay molecular structure, dynamics, biomechanics these wall-associated insoluble amorphous polymeric assemblies presents substantial investigative challenges. We report solid-state NMR coordinated FT-IR tensile strength measurements for periderms from...
The potato (Solanum tuberosum L.) ranks third in worldwide consumption among food crops. Whereas disposal of peels poses significant challenges for the industry, secondary metabolites these tissues are also bioactive and essential to crop development. diverse primary reported whole tubers wound-healing prompted a comprehensive profiling study native periderms from four cultivars with distinctive skin morphologies commercial uses. Polar nonpolar soluble were extracted concurrently, analyzed...
The cultivation, storage, and distribution of potato tubers are compromised by mechanical damage suboptimal healing. To investigate wound-healing progress in cultivars with contrasting russeting patterns, metabolite profiles reported previously for polar tissue extracts were complemented GC/MS measurements nonpolar quantitative (13)C NMR interfacial solid suspensions. Potential marker compounds that distinguish cultivar type time point included fatty acids, alcohols, alkanes, glyceryl...
Abstract The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem cork. Despite the vital importance of in protecting radially-growing plant organs wounded tissues, practically only suberin biosynthetic process has been studied molecularly so far. Since oak ( Quercus suber ) is seasonally activated its proliferation specification to a continuous developmental process, differentially expressed genes during seasonal growth served us identify molecular...