A5067650822- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Seed Germination and Physiology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Plant Genetic and Mutation Studies
- Archaeological and Historical Studies
- Medieval Architecture and Archaeology
- RNA Research and Splicing
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Micronutrient Interactions and Effects
- Phytase and its Applications
- Protein Kinase Regulation and GTPase Signaling
- Advanced Proteomics Techniques and Applications
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Fungal and yeast genetics research
- Mass Spectrometry Techniques and Applications
- Cellular transport and secretion
- Plant responses to water stress
Center for Research in Agricultural Genomics
2009-2016
Universitat Autònoma de Barcelona
1991-2014
Consejo Superior de Investigaciones Científicas
1988-2010
Centre d’Investigació i Desenvolupament
1997-2007
National Research Council
2006
Institut de Biologia Molecular de Barcelona
2001-2005
Centro de Investigación y Desarrollo
1987-2003
University of Girona
2000
Universitat de Lleida
1997
Instituto de Biología Molecular y Celular de Plantas
1996
Summary The abscisic acid‐responsive gene rab17 of maize is expressed during late embryogenesis, and induced by ABA desiccation in embryo vegetative tissues. ABRE DRE cis ‐elements are involved regulation the drought. Using yeast one‐hybrid screening, we isolated two cDNAs encoding new DRE‐binding proteins, designated DBF1 DBF2, that members AP2/EREBP transcription factor family. Analysis mRNA accumulation profiles showed embryogenesis after desiccation, NaCl treatments plant seedlings,...
The maize abscisic acid (ABA)-responsive rab17 mRNA and Rab17 protein distribution in embryo tissues was investigated by situ hybridization immunocytochemistry. were found all cells of tissues. Synthesis occurred initially the axis. As maturation progressed, detectable scutellum accumulated axis provascular However, response to exogenous ABA differed various cell types. located nucleus cytoplasm, qualitative differences phosphorylation states between two subcellular compartments. Based on...
The plasma membrane (PM) H(+)-ATPase has been proposed to play important transport and regulatory roles in plant physiology, including its participation auxin-induced acidification coleoptile segments. This enzyme is encoded by a family of genes differing tissue distribution, regulation, expression level. A major expressed isoform the maize PM (MHA2) characterized. RNA gel blot analysis indicated that MHA2 all organs, with highest levels being roots. In situ hybridization sections from...
Late Embryogenesis Abundant (LEA) proteins, a group of hydrophilic have been linked to survival in plants and animals periods stress, putatively through safeguarding enzymatic function prevention aggregation times dehydration/heat. Yet despite decades effort, the molecular-level mechanisms defining this protective remain unknown. In paper, we summarize review research discoveries classification LEA protein groups based on their amino acid sequence similarity presence distinctive conserved...
Lignin is an essential polymer in vascular plants that plays key structural roles vessels and fibers. Lignification induced by external inputs such as wounding, but the molecular mechanisms link this stress to lignification remain largely unknown. In work, we provide evidence three maize (Zea mays) lignin repressors, MYB11, MYB31, MYB42, participate wound-induced interacting with ZML2, a protein belonging TIFY family. We determined R2R3-MYB factors ZML2 bind vivo AC-rich GAT(A/C)...
We have determined the primary structure of a phospholipid transfer protein (PLTP) isolated from maize seeds. This consists 93 amino acids and shows internal homology originating in repetition (do)decapeptides. By using antibodies against PLTP, we cDNA library one positive clone (6B6) which corresponds to incomplete nucleotide sequence. Another (9C2) was obtained by screening size-selected with 6B6. Clone 9C2 (822 base pairs) full-length phospholipid-transfer whose mRNA contains 0.8...
The rab17 gene from maize is transcribed in late embryonic development and responsive to abscisic acid water stress embryo vegetative tissues. In vivo footprinting transient transformation of were performed embryos tissues characterize the cis ‐elements involved regulation gene. By footprinting, protein binding was observed nine elements promoter, which correspond five putative ABREs (abscisic elements) four other sequences. footprints indicated that distinct proteins interact with these two...
The maize abscisic acid responsive protein Rab17 is a highly phosphorylated late embryogenesis abundant involved in plant responses to stress. In this study, we provide evidence of the importance phosphorylation by kinase CK2 growth-related processes under stress conditions. We show specific interaction with regulatory subunits CK2β-1 and CK2β-3, that these interactions do not depend on state Rab17. Live-cell fluorescence imaging both indicates intracellular dynamics are regulated...
Mitogen-activated protein kinase (MAPK) cascades have important functions in plant stress responses and development are key players reactive oxygen species (ROS) signalling innate immunity. In Arabidopsis, the transmission of ROS pathogen by MAPKs involves coordinated activation MPK6 MPK3; however, specificity their negative regulation phosphatases is not fully known. Here, we present genetic analyses showing that MAPK phosphatase 2 (MKP2) regulates oxidative defence functionally interacts...
LEA (late embryogenesis abundant) proteins participate in plant stress tolerance responses, but the mechanisms by which protection occurs are not fully understood. In present work unfolded from maize dry embryos were analyzed mass spectrometry. Twenty embryo identified, and among them 13 corresponded to LEA-type proteins. We selected three major proteins, Emb564, Rab17 Mlg3, belonging groups 1, 2 3, respectively, we undertook a comparative study order highlight differences them. The...
Summary The maize abscisic acid‐responsive Rab17 protein localizes to the nucleus and cytoplasm in cells. In‐frame fusion of reporter β‐glucuronidase (GUS) directed GUS transgenic Arabidopsis thaliana transiently transformed onion Analysis chimeric constructs identified one region between amino acid positions 66–96, which was necessary for targeting nucleus. This contains a serine cluster followed by putative consensus site kinase CK2 phosphorylation, stretch basic acids resembling simian...
The maize gene RAB-17, which is responsive to abscisic acid, encodes a basic glycine-rich protein containing, in the middle part of its sequence, cluster 8 serine residues followed by putative casein kinase 2-type substrate consensus sequence.This was found be highly phosphorylated vivo.Here, we show that RAB-17 real for 2. vitro 2 isolated from rat liver cytosol and embryos.A maximum 4 mol phosphate were incorporated per following incubation with 2.Phosphopeptide mapping experiments peptide...
Summary A novel protein phosphatase in Arabidopsis thaliana was identified by database searching. This protein, designated AtPTPKIS1, contains a tyrosine (PTP) catalytic domain and kinase interaction sequence (KIS) domain. It is predicted to interact with plant SNF1‐related kinases (SnRKs), representing central regulators of metabolic stress responses. AtPTPKIS1 has close homologues other species, both dicots monocots, but not found kingdoms. The tomato homologue expressed as recombinant...