A5009167677- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Plant responses to water stress
- Phytase and its Applications
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Advanced Proteomics Techniques and Applications
- Allelopathy and phytotoxic interactions
- Crop Yield and Soil Fertility
- Enzyme-mediated dye degradation
- Protein Interaction Studies and Fluorescence Analysis
- Cassava research and cyanide
- Studies on Chitinases and Chitosanases
- Soybean genetics and cultivation
- Genomics and Phylogenetic Studies
University of Sfax
2014-2024
Centre of Biotechnology of Sfax
2014-2024
Institut Supérieur de Biotechnologie de Sfax
2003-2023
Département Biologie et Amélioration des Plantes
2017
Nagoya University
2011
Czech Academy of Sciences, Institute of Biotechnology
2011
University of Geneva
1997-2006
Friedrich Miescher Institute
2001
Transgenic Arabidopsis plants overexpressing the wheat vacuolar Na+/H+ antiporter TNHX1 and H+-PPase TVP1 are much more resistant to high concentrations of NaCl water deprivation than wild-type strains. These transgenic grow well in presence 200 mM also under a water-deprivation regime, while exhibit chlorosis growth inhibition. Leaf area decreased subjected salt or drought stress. The leaf potential was less negative for plants. This could be due an enhanced osmotic adjustment Moreover,...
Summary Precise modification by gene targeting (GT) provides an important tool for studies of function in vivo . Although routine with many organisms, only isolated examples GT events have been reported flowering plants. These were at low frequencies precluding reliable estimation efficiency and evaluation mechanisms. Here we present unambiguous straightforward system detection Arabidopsis using endogenous nuclear encoding protoporphyrinogen oxidase (PPO), involved chlorophyll heme...
The TIFY proteins constitute a plant-specific super-family and they are involved in regulating many plant processes, such as development, defences stress responses. Jasmonate-ZIM-Domain (JAZ) proteins, the best-characterized sub-group of family key regulator jasmonic acid (JA) signalling pathway. Jasmonates regulate several aspects play primary role defence mechanisms well responses to abiotic stresses. is studied dicots but poorly investigated monocots. present study reports an extensive...
We have previously reported that transgenic Arabidopsis plants overexpressing the wheat dehydrin DHN-5 show enhanced tolerance to osmotic stresses. In order understand mechanisms through which exerts this effect, we performed transcriptome profiling using Affymetrix ATH1 microarray. Our data an altered expression of 77 genes involved mainly in transcriptional regulation, cellular metabolism, stress and signaling. Among up-regulated genes, identified those are known be stress-related genes....
Group-2 late embryogenesis abundant (LEA) proteins, also known as dehydrins, are claimed to stabilize macromolecules against damage caused by freezing, dehydration, ionic or osmotic stresses. However, their precise function remains unknown. Here, we investigated the effect of wheat dehydrin (DHN-5) protein on activity and thermostability two distinct enzymes, beta-glucosidase (bglG) glucose oxidase/peroxidase (GOD/POD) in vitro. The purified DHN-5 had capacity preserve bglG subjected heat...
Phytic acid (PA) is a major source of inorganic phosphate (Pi) in the soil; however, plant lacks capacity to utilize it for Pi nutrition and growth. Microbial phytases constitute group enzymes that are able remobilize from PA. Thus, use these increase higher plants PA agronomical interest. In current study, we generate transgenic Arabidopsis lines (ePHY) overexpressing an extracellular form phytase PHY-US417 Bacillus subtilis, which characterized by high levels secreted activity. presence as...
Rhizobia secrete specific lipo‐chitooligosaccharide signals (LCOs) called Nod factors that are required for infection and nodulation of legumes. In Rhizobium sp. NGR234, the reducing N ‐acetyl‐ d ‐glucosamine LCOs is substituted at C 6 with 2‐ O ‐methyl‐ l ‐fucose which can be acetylated or sulphated. We identified a flavonoid‐inducible locus on symbiotic plasmid pNGR234 contains new gene, noeE sulphation NGR234 (NodNGR). was by conjugation into closely related fredii strain USDA257,...
Loci unique to specific rhizobia direct the adjunction of special groups core lipo-oligosaccharide Nod factors. Host-specificity ofnodulation (Hsn) genes are thus essential for interaction with certain legumes. <i>Rhizobium</i> sp. NGR234, which can nodulate >110 genera legumes, possesses three <i>hsn</i> loci and secretes a large family factors carrying substituents. Among them 3-<i>O</i> (or 4-<i>O</i>)- 6-<i>O</i>-carbamoyl groups, an <i>N</i>-methyl group, 2-<i>O</i>-methylfucose residue...
Transcriptional regulators of the lysR family largely control expression bacterial symbiotic genes. Rhizobium sp. NGR234 contains at least four members this family: two resemble nodD, while others are more closely related to syrM. Part extremely broad host range can be attributed nodD1, although second gene shares a high degree DNA sequence homology with nodD2 R. fredii USDA191. A mutant was constructed by insertional mutagenesis. This (NGR omega nodD2) deficient in nitrogen fixation on...
Rhizobium sp. strain NGR234 produces a flavonoid-inducible rhamnose-rich lipopolysaccharide (LPS) that is important for the nodulation of legumes. Many genes encoding rhamnan part molecule lie between 87 degrees and 110 pNGR234a, symbiotic plasmid NGR234. Computational methods suggest 5 12 open reading frames (ORFs) within this arc are involved in synthesis (and subsequent polymerization) L-rhamnose. Two others probably play roles transport carbohydrates. To evaluate function these ORFs, we...
Summary The Arabidopsis MIM gene encodes a protein belonging to the SMC family (structure maintenance of chromosomes) which is required for intrachromosomal homologous recombination (ICR). Both ICR and expression are enhanced by DNA‐damaging treatments, suggesting that factor limiting DNA repair (HR) under genotoxic stress. We tested this hypothesis measuring levels in mim mutant stress, using methyl methanesulfonate. Although clearly showed diminished basal induced ICR, enhancement...