Abstract Bacteriophages are ubiquitous viral predators that have primarily been studied using fast-growing laboratory cultures of their bacterial hosts. However, microbial life in nature is mostly a slow- or non-growing, dormant state. Here, we show diverse phages can infect deep-dormant bacteria and suspend replication until the host resuscitates (“hibernation”). newly isolated Pseudomonas aeruginosa phage, named Paride, directly replicate induce lysis While non-growing notoriously tolerant...

The genetic adaptation of pathogens in host tissue plays a key role the establishment chronic infections. While whole genome sequencing has opened up analysis changes occurring during long-term infections, identification and characterization adaptive traits is often obscured by lack knowledge underlying molecular processes. Our research addresses Pseudomonas aeruginosa small colony variant (SCV) morphotypes In lungs cystic fibrosis patients, appearance SCVs correlates with prolonged...

Over the past decades, pan-resistant strains of major bacterial pathogens have emerged and rendered clinically available antibiotics ineffective, putting at risk many achievements modern medicine, including surgery, cancer therapy, organ transplantation. A thorough understanding processes leading to development antibiotic resistance in human patients is thus urgently needed.

The ability of Ralstonia solanacearum to cause disease in plants depends on its type III secretion system (T3SS). expression the T3SS and effector substrates is coordinately controlled by a regulatory cascade, at bottom which HrpB. Transcription hrpB gene activated plant-responsive regulator named HrpG, master wide array pathogenicity functions R. solanacearum. We have identified genome strain GMI1000 close paralog hrpG (83% overall similarity protein level) that we prhG. Despite this high...

The flagellar motor is a sophisticated rotary machine facilitating locomotion and signal transduction. Owing to its important role in bacterial behavior, assembly activity are tightly regulated. For example, chemotaxis relies on sensory pathway coupling chemical information rotational bias of the through phosphorylation switch protein CheY. Using proteomics approach, we identified novel family CheY-like (Cle) proteins Caulobacter crescentus, which tune response binding second messenger...

C. canimorsus 5 has the capacity to grow at expenses of glycan moieties from host cells N-glycoproteins. Here, we show that also deglycosylate human IgG and analyze deglycosylation mechanism. We is achieved by a large complex spanning outer membrane consisting Gpd proteins sialidase SiaC. GpdD, -G, -E -F are surface-exposed lipoproteins. GpdDEF could contribute binding glycoproteins bacterial surface while GpdG endo-β-N-acetylglucosaminidase cleaving N-linked oligosaccharide after first...

When Caulobacter crescentus enters S-phase the replication initiation inhibitor CtrA dynamically positions to old cell pole be degraded by polar ClpXP protease. Polar delivery of requires PopA and diguanylate cyclase PleD that same pole. Here we present evidence originated through gene duplication from its paralogue response regulator subsequent co-option as c-di-GMP effector protein. While C-terminal catalytic domain (GGDEF) is activated phosphorylation N-terminal receiver domain,...

The α-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as model for the evolution bacterial pathogenesis. Vast abundance two particular phylogenetic lineages had been linked to enhanced host adaptability enabled by lineage-specific acquisition VirB/D4 type IV secretion system (T4SS) and parallel complex effector repertoires. However, limited availability genome sequences from one those well other, remote branches has so far hampered...

Summary Capnocytophaga canimorsus are commensal Gram‐negative bacteria from dog's mouth that cause rare but dramatic septicaemia in humans. C. have the unusual property to feed on cultured mammalian cells, including phagocytes, by harvesting glycan moiety of cellular glycoproteins. To understand mechanism behind this property, genome strain Cc5 was sequenced and analysed. In addition, were cultivated onto HEK 293 cells surface proteome determined. The found encode many lipoproteins encoded...

ABSTRACT Infections with the Gram-negative coccobacillus Acinetobacter baumannii are a major threat in hospital settings. The progressing emergence of multidrug-resistant clinical strains significantly reduces treatment options for clinicians to fight A. infections. current lack robust methods genetically manipulate drug-resistant isolates impedes research on resistance and virulence mechanisms clinically relevant strains. In this study, we developed highly efficient versatile genome-editing...

Capnocytophaga canimorsus are gram-negative bacteria living as commensals in the mouth of dogs and cats. C. cause rare but life-threatening generalized infections humans that have been contact with a dog or cat. Over last years we collected 105 strains from different geographical origins severe human healthy dogs. All these were analyzed by 16S rDNA sequencing phylogenetic tree revealed two main groups instead one no relation to origin. This branching was confirmed whole-genome 10 strains,...

Capnocytophaga canimorsus is a usual member of dog's mouths flora that causes rare but dramatic human infections after dog bites. We determined the structure C. lipid A. The main features are it penta-acylated and composed "hybrid backbone" lacking 4′ phosphate having 1 phosphoethanolamine (P-Etn) at 2-amino-2-deoxy-d-glucose (GlcN). LPS was 100 fold less endotoxic than Escherichia coli LPS. Surprisingly, A 20,000 A-core. This represents first example in which core-oligosaccharide...

ABSTRACT Capnocytophaga canimorsus , a dog mouth commensal and member of the Bacteroidetes phylum, causes rare but often fatal septicemia in humans that have been contact with dog. Here, we show C. strains isolated from human infections grow readily heat-inactivated serum this property depends on typical polysaccharide utilization locus (PUL), namely, PUL3 strain Cc5. PUL are hallmark they encode various products, including surface protein complexes capture process polysaccharides or...

ABSTRACT Capnocytophaga canimorsus is known to form two kinds of cells on blood agar plates (coccoid and bacillary), evoking phase variation. When grown in coculture with animal these bacteria appeared only as bacilli, but the presence vancomycin they were round, indicating that coccoid shapes likely result from weakening peptidoglycan layer. Polysaccharide utilization locus 5 ( PUL5 ) sialidase mutant bacteria, unable retrieve glycans glycoproteins, grew less than wild-type also polymorphic...

Abstract Despite mounting evidence that in clonal bacterial populations, phenotypic variability originates from stochasticity gene expression, little is known about noise-shaping evolutionary forces and how expression noise translates to differences. Here we developed a high-throughput assay uses redox-sensitive dye couple growth of thousands colonies their respiratory activity show Escherichia coli , noisy regulation lower glycolysis citric acid cycle responsible for large variations...

Capnocytophaga canimorsus is a commensal Gram-negative bacterium, originally isolated from dog's mouth, that causes septicemia in humans. C. has the unusual ability to feed on host cells, including phagocytes. This capacity depends surface-exposed glycan-foraging systems. Here we present first complete genome sequence of strain (Cc5).

To investigate retinal involvement in chronic Chagas' disease, we performed electroretinography and fluorescein angiography studies chagasic patients. Our results demonstrated a dissociated electrophysiological response characterized by both an abnormal reduction of the electroretinographic b-wave amplitude delayed latency, under dark-adaptated condition. These alterations are compatible with selective dysfunction rods. Antibodies raised against Trypanosoma cruzi that also interact...

Capnocytophaga canimorsus is a bacterium from the normal oral flora of dogs and cats that causes rare generalized infections in humans. In an attempt to determine whether could be caused by subset strains identify pathogenicity factors, we sequenced genomes three isolated human infections.

Here, we present the draft genome sequences of three strains Capnocytophaga canimorsus, each isolated from a different dog's mouth. Genome analysis provided evidence that these organisms may belong to nonpathogenic subtype C. canimorsus.