A5051468623- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Neuroscience and Neuropharmacology Research
- Biochemical and Molecular Research
- Neuropeptides and Animal Physiology
- Plant and Fungal Interactions Research
- Bacterial Genetics and Biotechnology
- Receptor Mechanisms and Signaling
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Antibiotic Resistance in Bacteria
- Herpesvirus Infections and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Escherichia coli research studies
- Anesthesia and Neurotoxicity Research
- Computational Drug Discovery Methods
- Metabolomics and Mass Spectrometry Studies
- Anesthesia and Sedative Agents
Albert Einstein College of Medicine
2020-2024
Maurice Wilkins Centre
2017-2022
University of Auckland
2017-2022
University of Waikato
2013-2022
Mycobacterium tuberculosis (Mtb) is a globally distributed bacterial pathogen whose population structure has largely been shaped by the activities of its obligate human host. Oceania was last major global region to be reached Europeans and for which dispersal evolution Mtb remains unexplored. Here, we investigated evolutionary history Euro-American L4.4 sublineage South Pacific. Using phylodynamics approach dataset 236 genomes have traced origins strains New Zealand. These are predominantly...
Phenotypic testing for drug susceptibility of Mycobacterium tuberculosis is critical to basic research and managing the evolving problem antimicrobial resistance in management, but it remains a specialized technique which access severely limited. Here, we report on development validation an improved phage-mediated detection system M. We incorporated nanoluciferase (Nluc) reporter gene cassette into TM4 mycobacteriophage genome create phage TM4-nluc. assessed performance this context cellular...
Genome sequencing and assembly of viral genomes within the
Drug-resistant tuberculosis is a growing public health threat, and early characterization of the resistance phenotype essential for guiding treatment mitigating high mortality associated with disease. However, slow growth rate Mycobacterium tuberculosis, causative agent necessitates several weeks conventional culture-dependent drug susceptibility testing (DST). In addition, there are no widely available molecular diagnostic assays evaluating to newer drugs or complex mechanisms.
Abstract Phthiocerol dimycocerosate (PDIM) is an essential virulence lipid of Mycobacterium tuberculosis . In vitro culturing rapidly selects for spontaneous mutations that cause PDIM loss leading to attenuation and increased cell wall permeability. We discovered due a metabolic deficiency methylmalonyl-CoA impedes the growth PDIM-producing bacilli. This can be remedied by supplementation with odd-chain fatty acids, cholesterol, or vitamin B 12 developed much-needed facile scalable routine...
Abstract The Mycobacterium tuberculosis complex lineage 4 (L4), also known as the “Euro-American” lineage, is most widely dispersed of seven human adapted lineages. L4 comprised ten sublineages including L4.4, which has a moderate global distribution and common sublineage in New Zealand. We have used phylodynamics approach dataset 236 M. genomes to trace origins dispersal L4.4 strains Zealand that are predominantly found Māori Pacific people. identify an L4.4.1.1 clade European origin,...
We report here the complete genome sequence of Mycobacterium tuberculosis strain Colonial S-type 1 (CS1), which has been responsible for ongoing outbreaks in New Zealand over past 30 years. CS1 appears to be highly transmissible, with greater rates progression active disease, compared other circulating M. strains; therefore, comparison its genomic content is interest.