A5043003130- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Sulfur Compounds in Biology
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Microplastics and Plastic Pollution
- Effects and risks of endocrine disrupting chemicals
- Infectious Diseases and Tuberculosis
- Polyamine Metabolism and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- interferon and immune responses
- Inhalation and Respiratory Drug Delivery
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacteriophages and microbial interactions
Africa Health Research Institute
2020-2024
University of KwaZulu-Natal
2021-2024
Stellenbosch University
2018
Abstract Hydrogen sulfide (H 2 S) is involved in numerous pathophysiological processes and shares overlapping functions with CO • NO. However, the importance of host-derived H S microbial pathogenesis unknown. Here we show that Mtb -infected mice deficient S-producing enzyme cystathionine β-synthase (CBS) survive longer reduced organ burden, pharmacological inhibition CBS reduces bacillary load mice. High-resolution respirometry, transcriptomics mass spectrometry establish stimulates...
H2S is a potent gasotransmitter in eukaryotes and bacteria. Host-derived has been shown to profoundly alter M. tuberculosis (Mtb) energy metabolism growth. However, compelling evidence for endogenous production of its role Mtb physiology lacking. We show that multidrug-resistant drug-susceptible clinical strains produce H2S, whereas non-pathogenic smegmatis barely detectable. identified Rv3684 (Cds1) as an H2S-producing enzyme cds1 disruption reduces, but does not eliminate, production,...
Abstract Potential endocrine disrupting chemicals (EDCs) are present in bottled water from various countries. In South Africa (SA), increased consumption and concomitant increases plastic packaging create important consequences for public health. This study aimed to screen SA estrogenic activity, selected target assessing potential health risks. Ten brands were exposed 20 °C 40 over 10 days. Estrogenic activity was assessed using the recombinant yeast estrogen (YES) T47D-KBluc reporter gene...
Abstract Mycobacterium tuberculosis (Mtb) is one of the most successful human pathogens. Several cytokines are known to increase virulence bacterial pathogens, leading us investigate whether Interferon-γ (IFN-γ), a central regulator immune defense against Mtb , has direct effect on bacteria. We found that recombinant and T-cell derived IFN-γ rapidly induced dose-dependent in oxygen consumption rate (OCR) consistent with increased respiration. This was not observed attenuated Bacillus...
Abstract H 2 S is a potent gasotransmitter in eukaryotes and bacteria. Host-derived has been shown to profoundly alter M. tuberculosis ( Mtb ) energy metabolism growth. However, compelling evidence for endogenous production of its role physiology lacking. We show that multidrug-resistant drug-susceptible clinical strains produce S, whereas non-pathogenic smegmatis barely detectable. identified Rv3684 (Cds1) as an S-producing enzyme cds1 disruption reduces, but does not eliminate, production,...
Drug-resistant tuberculosis is a growing public health threat, and early characterization of the resistance phenotype essential for guiding treatment mitigating high mortality associated with disease. However, slow growth rate Mycobacterium tuberculosis, causative agent necessitates several weeks conventional culture-dependent drug susceptibility testing (DST). In addition, there are no widely available molecular diagnostic assays evaluating to newer drugs or complex mechanisms.
Abstract Mycobacterium tuberculosis (Mtb) is one of the most successful human pathogens and remains a leading cause death from infectious disease. Interferon-γ (IFN-γ) central regulator immune defense against Mtb . Several cytokines have been shown to increase virulence other bacterial pathogens, us investigate whether IFN-γ has direct effect on We found that both recombinant T-cell derived rapidly induced dose-dependent in oxygen consumption rate (OCR) , consistent with increased...