A5086687563- Tuberculosis Research and Epidemiology
- Inhalation and Respiratory Drug Delivery
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Metabolomics and Mass Spectrometry Studies
- Cancer therapeutics and mechanisms
- Sulfur Compounds in Biology
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Heme Oxygenase-1 and Carbon Monoxide
- Ion channel regulation and function
- Thermal Regulation in Medicine
- Hydrological Forecasting Using AI
- Trace Elements in Health
- Amino Acid Enzymes and Metabolism
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Polyamine Metabolism and Applications
- Lipid Membrane Structure and Behavior
- Silymarin and Mushroom Poisoning
- Iron Metabolism and Disorders
- Inflammation biomarkers and pathways
- Phenothiazines and Benzothiazines Synthesis and Activities
- Entomopathogenic Microorganisms in Pest Control
- Parasites and Host Interactions
- Insect Resistance and Genetics
University of Alabama at Birmingham
2015-2024
Indian Institute of Oilseeds Research
2012
Highlights•WhiB3 regulates EGT production and maintains bioenergetic homeostasis in Mtb•EGT modulates drug sensitivity protects Mtb from diverse oxidative stressors•EGT is essential for survival macrophages miceSummaryThe mechanisms by which Mycobacterium tuberculosis (Mtb) metabolic equilibrium to survive during infection upon exposure antimycobacterial drugs are poorly characterized. Ergothioneine (EGT) mycothiol (MSH) the major redox buffers present Mtb, but contribution of virulence...
Abstract Hydrogen sulfide (H 2 S) is involved in numerous pathophysiological processes and shares overlapping functions with CO • NO. However, the importance of host-derived H S microbial pathogenesis unknown. Here we show that Mtb -infected mice deficient S-producing enzyme cystathionine β-synthase (CBS) survive longer reduced organ burden, pharmacological inhibition CBS reduces bacillary load mice. High-resolution respirometry, transcriptomics mass spectrometry establish stimulates...
Iron is an essential factor for the growth and virulence of Mycobacterium tuberculosis (Mtb). However, little known about mechanisms by which host controls iron availability during infection. Since ferritin heavy chain (FtH) a major intracellular source reserve in host, we hypothesized that lack FtH would cause dysregulated homeostasis to exacerbate TB disease. Therefore, used knockout mice lacking myeloid-derived cell populations study Mtb disease progression. We found plays critical role...
Abstract Accurate lipid annotation is crucial for understanding the role of lipids in health and disease identifying therapeutic targets. However, annotating wide variety species biological samples remains challenging untargeted lipidomic studies. In this work, we present a workflow based on LC-MS MS/MS strategies, combination four bioinformatic tools, decision tree to support accurate semi-quantification lung tissue from control mice. The proposed allowed us generate lung-based ATLAS...
Heme oxygenase-1 (HO-1) is a cytoprotective enzyme that controls inflammatory responses and redox homeostasis; however, its role during pulmonary tuberculosis (TB) remains unclear. Using freshly resected human TB lung tissue, we examined the of HO-1 within cellular pathological spectrum TB. Flow cytometry histopathological analysis tissues showed expressed primarily in myeloid cells levels these were directly proportional to cytoprotection. mitigates pathophysiology by diminishing...
The mechanisms whereby
Abstract Mycobacterium tuberculosis ( Mtb ) disrupts glycolytic flux in infected myeloid cells through an unclear mechanism. Flux the pathway is inextricably linked to availability of NAD + , which maintained by salvage and lactate metabolism. Using lung tissue from (TB) patients deficient LDHA Ldha LysM−/− mice, we demonstrate that glycolysis essential for protective immunity TB. Glycolytic are early recruitment multiple classes immune IFNγ-mediated protection. We identify depletion as...
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, encounters variable and hostile environments within host. A major component these conditions is reductive oxidative stresses induced by factors modified host immune response, such as oxygen tension, NO or CO gases, reactive nitrogen intermediates, availability different carbon sources changes in pH. It therefore essential for Mtb to continuously monitor appropriately respond microenvironment. To this end, has developed...
Mycobacterium tuberculosis (Mtb) senses and responds to host-derived gasotransmitters NO CO via heme-containing sensor kinases DosS DosT the response regulator DosR. Hydrogen sulfide (H2S) is an important signaling molecule in mammals, but its role Mtb physiology unclear. We have previously shown that exogenous H2S can modulate expression of genes Dos dormancy regulon unknown mechanism(s). Here, we test hypothesis DosS/T/R system. Using UV–Vis EPR spectroscopy, show binds directly ferric...
Abstract Mycobacterium tuberculosis ( Mtb ) disrupts glycolytic flux in infected myeloid cells through an unclear mechanism. Flux the pathway is inextricably linked to availability of NAD + , which maintained by salvage and lactate metabolism. Using lung tissue from (TB) patients deficient LDHA Ldha LysM−/− mice, we demonstrate that glycolysis essential for protective immunity TB. Glycolytic are early recruitment multiple classes immune effects IFNγ. We identified depletion as central...
Abstract Accurate lipid annotation is crucial for understanding the role of lipids in health and disease identifying therapeutic targets. However, annotating wide variety species biological samples remains challenging untargeted lipidomic studies. In this work, we present an optimized workflow based on combination LC-MS MS/MS strategies, four bioinformatic tools, a decision-tree-based approach to support accurate semi-quantification lung tissue from control mice. The developed allowed us...
Abstract Mycobacterium tuberculosis ( Mtb ) senses and responds to host-derived gasotransmitters NO CO via heme-containing sensor kinases DosS DosT the response regulator DosR. Hydrogen sulfide (H 2 S) is an important signaling molecule in mammals, but its role physiology unclear. We have previously shown that exogenous H S can modulate expression of genes Dos dormancy regulon unknown mechanism(s). Here, we tested hypothesis DosS/T/R system. Using UV-Vis EPR spectroscopy, show binds directly...