A5007159635- Chronic Myeloid Leukemia Treatments
- Quinazolinone synthesis and applications
- Protein Structure and Dynamics
- Eosinophilic Disorders and Syndromes
- 14-3-3 protein interactions
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Protein Kinase Regulation and GTPase Signaling
- Chronic Lymphocytic Leukemia Research
- Bacterial Genetics and Biotechnology
- Molecular spectroscopy and chirality
- Synthesis and Characterization of Heterocyclic Compounds
- Advanced NMR Techniques and Applications
- Chemical Synthesis and Analysis
- Toxin Mechanisms and Immunotoxins
- Heat shock proteins research
- Protein Tyrosine Phosphatases
- Phagocytosis and Immune Regulation
- Electron Spin Resonance Studies
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- PI3K/AKT/mTOR signaling in cancer
- Glycosylation and Glycoproteins Research
- Spectroscopy and Quantum Chemical Studies
University of Basel
2009-2024
National Institute of Pharmaceutical Education and Research
2023-2024
ETH Zurich
2023-2024
Max Planck Research Unit for Enzymology of Protein Folding
2002
Harvard University
1996
Max Planck Institute of Biochemistry
1991-1992
Max Planck Society
1991-1992
Cyclic diguanosine monophosphate (c-di-GMP) is a ubiquitous bacterial second messenger that controls the switch from single-cell lifestyle to surface-attached, multicellular communities called biofilms. PilZ domain proteins are family of c-di-GMP receptors, which control various cellular processes. We have solved solution structure Pseudomonas aeruginosa single-domain protein PA4608 in complex with by NMR spectroscopy. Isotope labeling 13C and 15N both ligand made it possible define at high...
The flagellar motor is a sophisticated rotary machine facilitating locomotion and signal transduction. Owing to its important role in bacterial behavior, assembly activity are tightly regulated. For example, chemotaxis relies on sensory pathway coupling chemical information rotational bias of the through phosphorylation switch protein CheY. Using proteomics approach, we identified novel family CheY-like (Cle) proteins Caulobacter crescentus, which tune response binding second messenger...
Significance Cyclic diguanylate (c-di-GMP) regulates a wide range of bacterial cellular functions from biofilm formation to growth and survival. Based on the structural analysis complex c-di-GMP with effector protein followed by amino acid sequence optimization, we have developed short peptide that binds nanomolar affinity high specificity. This provides many opportunities for biotechnological biomedical applications. In particular, show such an endogenously expressed effectively reduces...
It was recently reported (Xie et al., 2022) that the Abelson tyrosine kinase (Abl) ATP-site inhibitor imatinib also binds to Abl's myristoyl binding pocket, which is target of allosteric Abl inhibitors. This based on a crystal structure truncated domain construct in complex with bound site as well further isothermal titration calorimetry (ITC), NMR, and activity data. Although imatinib's affinity for significantly weaker (10
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructures of proteins in solution derived from homonuclear three-dimensional NOE-NOE nuclear magnetic resonance spectroscopy. High-resolution structure squash trypsin inhibitorTad A. Holak, J. Habazettl, H. Oschkinat, and OtlewskiCite this: Am. Chem. Soc. 1991, 113, 8, 3196–3198Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April...
Significance Multidrug recognition is an important phenomenon that not well understood. TipA, a bacterial transcriptional regulator, constitutes minimal multidrug resistance system against numerous thiopeptide antibiotics. We show motions in the millisecond to microsecond time range form basis of TipA mechanism. This may be common many systems. The discovery structural antibiotic motifs essential for binding and ribosome are identical makes mechanism useful model ribosomal current drug development.
The 10 kDa amino-terminal fragment of Escherichia coli Ada protein (N-Ada10) repairs methyl phosphotriesters in DNA and possesses a tightly bound zinc ion. complete resonance assignments this domain have been obtained using multidimensional homonuclear heteronuclear NMR experiments. served to study the internal mobility via 15N relaxation This involved measurement longitudinal transverse rates, as well amide proton solvent exchange rates. Relaxation rates rotating frame, R1ρ, nuclei were...
Abelson tyrosine kinase (Abl) is regulated by the arrangement of its regulatory core, consisting sequentially SH3, SH2, and (KD) domains, where an assembled or disassembled core corresponds to low high activity, respectively. It was recently established that binding type II ATP site inhibitors, such as imatinib, generates a force from KD N-lobe onto SH3 domain in consequence disassembles core. Here, we demonstrate C-terminal αI-helix exerts additional toward SH2 domain, which correlates both...
* Corresponding author Two functionally redundant enzymes, trigger and the hsp70 chaperone DnaK, have been assist de novo protein folding in E. coli. Trigger is a peripheral peptidyl prolyl cis/trans isomerase (PPIase) of large subunit ribosome. contrast, DnaK displays two catalytic features: secondary amide peptide bond (APIase) function supplemented by ATPase APIases accelerate isomerization nonprolyl bonds. Both enzymes for an unfolded polypeptide chain. The diminished temperature cell...
Abelson tyrosine kinase (Abl) is regulated by the arrangement of its regulatory core, consisting sequentially SH3, SH2 and (KD) domains, where an assembled or disassembled core corresponds to low high activity, respectively. It was recently established that binding type II ATP site inhibitors, such as imatinib, generates a force from KD N-lobe onto SH3 domain in consequence disassembles core. Here, we demonstrate C-terminal αI-helix exerts additional towards domain, which correlates both...
Abelson tyrosine kinase (Abl) is regulated by the arrangement of its regulatory core, consisting sequentially SH3, SH2, and (KD) domains, where an assembled or disassembled core corresponds to low high activity, respectively. It was recently established that binding type II ATP site inhibitors, such as imatinib, generates a force from KD N-lobe onto SH3 domain in consequence disassembles core. Here, we demonstrate C-terminal αI-helix exerts additional toward SH2 domain, which correlates both...