A5049317360- Legionella and Acanthamoeba research
- Vibrio bacteria research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacterial biofilms and quorum sensing
- Autophagy in Disease and Therapy
- Inhalation and Respiratory Drug Delivery
- Enterobacteriaceae and Cronobacter Research
- Pediatric health and respiratory diseases
- Lipid Membrane Structure and Behavior
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Neonatal Health and Biochemistry
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cellular transport and secretion
- Biomedical Research and Pathophysiology
- Mitochondrial Function and Pathology
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Heme Oxygenase-1 and Carbon Monoxide
- Parasitic Infections and Diagnostics
- Viral gastroenteritis research and epidemiology
- Endoplasmic Reticulum Stress and Disease
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- RNA Interference and Gene Delivery
University of Basel
2023-2024
Bioengineering Center
2024
University of Zurich
2017-2023
Osnabrück University
2012-2017
Abstract Legionella pneumophila can cause Legionnaires’ disease and replicates intracellularly in a distinct -containing vacuole (LCV). LCV formation is complex process that involves plethora of type IV-secreted effector proteins. The RidL binds the Vps29 retromer subunit, blocks retrograde vesicle trafficking, promotes intracellular bacterial replication. Here, we reveal 29-kDa N-terminal domain (RidL 2–281 ) adopts “foot-like” fold comprising protruding β-hairpin at its “heel”. deletion...
The amoeba-resistant bacterium Legionella pneumophila causes Legionnaires’ disease and employs a type IV secretion system (T4SS) to replicate in the unique, ER-associated -containing vacuole (LCV). large fusion GTPase Sey1/atlastin is implicated ER dynamics, ER-derived lipid droplet (LD) formation, LCV maturation. Here, we employ cryo-electron tomography, confocal microscopy, proteomics, isotopologue profiling analyze LCV-LD interactions genetically tractable amoeba Dictyostelium discoideum...
Legionella pneumophila is a ubiquitous environmental bacterium which, upon inhalation, causes life-threatening pneumonia termed Legionnaires’ disease. The opportunistic pathogen grows in amoebae and macrophages by employing “type IV” secretion system, which secretes more than 300 different “effector” proteins into the host cell, where they subvert pivotal processes. function of many these effector unknown, their evolution has not been studied. L. RCC1 repeat effectors target small GTPase...
Dictyostelium discoideum Sey1 is the single ortholog of mammalian atlastin 1-3 (ATL1-3), which are large homodimeric GTPases mediating homotypic fusion endoplasmic reticulum (ER) tubules. In this study, we generated a D. mutant strain lacking sey1 gene and found that amoebae deleted for enlarged, but grow develop similarly to parental strain. The ∆sey1 showed an altered ER architecture, tubular network was partially disrupted without any major consequences other organelles or architecture...
Abstract Transwell‐based airway models have become increasingly important in studying the effects of respiratory diseases and drug treatment at air–liquid interface lung epithelial barrier. However, underlying mechanisms tissue cell level often remain unclear, as transwell inserts feature limited live‐cell imaging compatibility. Here, a novel microfluidic platform is reported for cultivation transwell‐based tissues providing possibility to alternate between liquid–liquid interfaces. While...
Summary While commensal bacteria generally respect natural barriers of the human body, pathogens are able to breach epithelia, invade deeper tissue layers and cause life-threatening infections. Pseudomonas aeruginosa , an opportunistic pathogen, is a leading severe hospital-acquired pneumonia, with mortality rates as high 50% in mechanically ventilated patients 1–3 . Effective colonization breaching lung mucosa hallmarks P. pathogenesis 4 Although virulence factors behavioral strategies have...
Abstract Transwell-based airway models have become increasingly important to study the effects of respiratory diseases and drug treatment at air-liquid interface lung epithelial barrier. However, underlying mechanisms tissue cell level often remain unclear, as transwell inserts feature limited live-cell imaging compatibility. Here, we report on a novel microphysiological platform for cultivation transwell-based tissues providing possibility alternate between liquid-liquid interfaces. While...
Abstract The facultative intracellular bacterium Legionella pneumophila employs the Icm/Dot type IV secretion system (T4SS) to replicate in a unique membrane-bound compartment, -containing vacuole (LCV). endoplasmic reticulum (ER)-resident large fusion GTPase Sey1/atlastin promotes remodeling and expansion of LCVs, is also implicated formation ER-derived lipid droplets (LDs). Here we show that LCVs intimately interact with palmitate-induced LDs Dictyostelium discoideum amoeba. Comparative...