A5020356066- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Vibrio bacteria research studies
- Amino Acid Enzymes and Metabolism
- Antibiotic Resistance in Bacteria
- Immune Cell Function and Interaction
- Microbial Metabolic Engineering and Bioproduction
- T-cell and B-cell Immunology
- Gut microbiota and health
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- DNA Repair Mechanisms
- Plant and fungal interactions
- Renal cell carcinoma treatment
- Melanoma and MAPK Pathways
- Allergic Rhinitis and Sensitization
- Metabolomics and Mass Spectrometry Studies
- Mental Health Treatment and Access
- Biochemical and Structural Characterization
University of Basel
2008-2023
University Hospital of Basel
2022
Sigmund Freud University Vienna
2022
ETH Zurich
2017
University of Konstanz
2004-2008
During long-term cystic fibrosis lung infections, Pseudomonas aeruginosa undergoes genetic adaptation resulting in progressively increased persistence and the generation of adaptive colony morphotypes. This includes small variants (SCVs), auto-aggregative, hyper-adherent cells whose appearance correlates with poor function infection. The SCV morphotype is strongly linked to elevated levels cyclic-di-GMP, a ubiquitous bacterial second messenger that regulates transition between motile...
The genetic adaptation of pathogens in host tissue plays a key role the establishment chronic infections. While whole genome sequencing has opened up analysis changes occurring during long-term infections, identification and characterization adaptive traits is often obscured by lack knowledge underlying molecular processes. Our research addresses Pseudomonas aeruginosa small colony variant (SCV) morphotypes In lungs cystic fibrosis patients, appearance SCVs correlates with prolonged...
ABSTRACT Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen and threat for immunocompromised cystic fibrosis patients. It responsible acute chronic infections can switch between these lifestyles upon taking an informed decision involving complex regulatory networks. The RetS/LadS/Gac/Rsm network the cyclic-di-GMP (c-di-GMP) signaling pathways are both central to this phenomenon redirecting P. population toward biofilm mode of growth, which associated with infections....
Mucosal-associated invariant T (MAIT) cells are the most abundant in human liver. They respond to bacterial metabolites presented by major histocompatibility complex-like molecule MR1. MAIT exert regulatory and antimicrobial functions implicated liver fibrogenesis. It is not well understood which function as antigen (Ag)-presenting for cells, under conditions stimulatory Ags reach circulation.We used different types of primary Ag-presentation assays blood-derived liver-derived cells. We...
ABSTRACT MurQ is an N -acetylmuramic acid-phosphate (MurNAc-P) etherase that converts MurNAc-P to -acetylglucosamine-phosphate and essential for growth on MurNAc as the sole source of carbon (T. Jaegar, M. Arsic, C. Mayer, J. Biol. Chem. 280: 30100-30106, 2005). Here we show only in Escherichia coli AnmK kinase are required utilization anhydro-MurNAc derived either from cell wall murein or imported medium.
The ubiquitous bacterial cell wall sugar N-acetylmuramic acid (MurNAc) carries a unique d-lactyl ether substituent at the C3 position. Recently, we proposed an etherase capable of cleaving this lactyl to be part novel MurNAc dissimilation pathway (Dahl, U., Jaeger, T., Nguyen, B. Sattler, J. M., Mayer, C. (2004) Bacteriol. 186, 2385–2392). Here, report identification first known etherase. encoding gene murQ is located 55 min on Escherichia coli chromosome adjacent murP, MurNAc-specific...
We report here that wild-type Escherichia coli grows on N-acetylmuramic acid (MurNAc) as the sole source of carbon and energy. Analysis mutants defective in N-acetylglucosamine (GlcNAc) catabolism revealed catabolic pathway for MurNAc merges into GlcNAc level 6-phosphate. Furthermore, analysis components phosphotransferase system (PTS) a PTS is essential growth MurNAc. However, neither glucose-, mannose/glucosamine-, nor GlcNAc-specific (PtsG, ManXYZ, NagE, respectively) was found to be...
ABSTRACT The MurNAc etherase MurQ of Escherichia coli is essential for the catabolism bacterial cell wall sugar N -acetylmuramic acid (MurNAc) obtained either from environment or endogenous (i.e., recycling). High-level expression murQ required growth on as sole source carbon and energy, whereas constitutive low-level sufficient recycling peptidoglycan fragments continuously released during bacteria. Here we characterize first time its regulation by MurR, a member poorly characterized...
Current antibiotics gradually lose their efficacy against chronic Pseudomonas aeruginosa infections due to development of increased resistance mediated by biofilm formation, as well the large arsenal microbial virulence factors that are coordinated cell density-dependent phenomenon quorum sensing. Here, we address this issue using synthetic biology principles rationally engineer quorum-quencher cells with closed-loop control autonomously dampen and interfere integrity. Pathogen-derived...
Background: The media are an important source of information on mental health. They often implicit in reinforcing negative stereotypes people with health problems. There no studies German-speaking countries or Russia attitudes to and professionals’ (MHP) the media. Aims: This study explored journalists MHPs reporting German speaking Switzerland, Germany, Austria Russia. Methods: A cross-sectional online internet survey, ten Likert scale statements ascertain perceptions stigma, role, training...