A5034949708- Cholesterol and Lipid Metabolism
- Antioxidant Activity and Oxidative Stress
- Peroxisome Proliferator-Activated Receptors
- Hormonal and reproductive studies
- Hormonal Regulation and Hypertension
- Steroid Chemistry and Biochemistry
- Lipid metabolism and biosynthesis
- Aquaculture disease management and microbiota
- Atherosclerosis and Cardiovascular Diseases
- Force Microscopy Techniques and Applications
- Free Radicals and Antioxidants
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Nitrogen and Sulfur Effects on Brassica
- Electrochemical sensors and biosensors
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Molecular Research
- Receptor Mechanisms and Signaling
- Electrochemical Analysis and Applications
- Biochemical effects in animals
- Genomics, phytochemicals, and oxidative stress
- Analytical Chemistry and Sensors
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Eicosanoids and Hypertension Pharmacology
Hokkaido University
2013-2025
Sapporo University
2012-2019
Health Sciences University of Hokkaido
2005-2014
National Institutes of Health
1999-2012
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2001-2008
Health and Human Development (2HD) Research Network
2003
Hokkaido University Hospital
2002
Experimental Station
1994
In humans, the biotransformation of cholesterol and its hydroxylated metabolites (oxysterols) by sulfonation is a fundamental process great importance. Nevertheless, sulfotransferase enzyme(s) that carries out this function has never been clearly identified. Cholesterol relatively poor substrate for previously cloned hydroxysteroid (HST), i.e. dehydroepiandrosterone (DHEA) (HST1). Recently, cloning single human gene encodes two proteins related to HST1 was reported. These newly...
Oxysterols constitute a class of cholesterol derivatives that exhibit broad biological effects ranging from cytotoxicity to regulation nuclear receptors. The role oxysterols such as 7-ketocholesterol (7-KC) in the development retinal macular degeneration and atheromatous lesions is particular interest, but little known their metabolic fate. We establish steroid/sterol sulfotransferase SULT2B1b, efficiently sulfonate cholesterol, also effectively sulfonates variety oxysterols, including 7-KC....
Using an oxygen radical absorbance capacity (ORAC) assay, antioxidant activity was detected in the ethanol extract of Pacific oyster, which purified by sequential extraction with organic solvents. The ethyl acetate fraction showed strongest and further purified, yielding a single compound [as assessed thin-layer chromatography (TLC) reverse-phase high-performance liquid (HPLC)]. This identified as 3,5-dihydroxy-4-methoxybenzyl alcohol on basis 1H 13C nuclear magnetic resonance (NMR),...
The gene for human hydroxysteroid sulfotransferase (SULT2B1) encodes two peptides, SULT2B1a and SULT2B1b, that differ only at their amino termini. SULT2B1b has a predilection cholesterol but is also capable of sulfonating pregnenolone, whereas preferentially sulfonates pregnenolone minimally cholesterol. We have determined the crystal structure bound to substrate donor product 3′-phosphoadenosine 5′-phosphate 2.9 2.4 Å, respectively, as well in presence acceptor 2.3 Å. These structures...
Branched fatty acid esters of hydroxy acids (FAHFAs) are a recently discovered class biologically active lipids with anti-inflammatory and anti-diabetic properties. Despite the possible link between endogenous FAHFA levels nuclear factor erythroid 2-related 2 (Nrf2), their function as antioxidants mechanisms involved in this unknown. Here, we investigate FAHFAs' plausible antioxidant potential reference to effect on Nrf2 levels, oxidative stress, lipid droplet oxidation human hepatocytes...
Sulphonation, a fundamental process essential for normal growth and development, requires the sulphonate donor molecule 3'-phosphoadenosine 5'-phosphosulphate (PAPS), which is produced from ATP inorganic sulphate by bifunctional enzyme PAPS synthase. In humans, two genes encode isoenzymes that are 77% identical at amino acid level, alternative splicing creates subtypes of synthase 2. The question as to whether distinctions in composition reflected differences activity has been examined....
Serotonin (5-HT), the endogenous nonselective 5-HT receptor agonist, activates inositol 1,4,5-triphosphate/calcium (InsP3/Ca2+) signaling pathway and exerts both stimulatory inhibitory actions on cAMP production GnRH release in immortalized neurons. The high degree of similarity between secretory responses elicited by prompted us to target specific subtypes deconvolute complex these agonists signal transduction release. Specific mRNA transcripts for 5-HT1A, 5-HT2C, 5-HT4, 5-HT7 were...
Excessive reactive oxygen species (ROS) levels cause oxidative stress, which can lead to various diseases. Renal failure is associated with stress and mitochondrial dysfunction. Vitamin K1 (phylloquinone) K2 (menaquinone) are essential for blood coagulation bone formation. K has been shown have anti-inflammation, glucose metabolism regulation, antiferroptosis functions. We investigated the impact of menaquinone-4 (MK-4) on dysfunction in human renal proximal tubular cells. MK-4 protected...
A novel mouse hydroxysteroid sulfotransferase cDNA has been cloned, and organization of its gene structure determined. The new sulfotransferase, SULT2B1a, closely related isoform, SULT2B1b, are derived from a single SULT2B1 as result an alternative exon I differential splicing. Thus, the only structural distinction between two isoforms is at their amino-terminal ends. Importantly, in contrast to prototypical SULT2A1, have predilection for cholesterol. Real-time RT-PCR reveals that SULT2B1a...
Summary CD36 deficiency was studied with attention to the phenotypegenotype relationship. The diagnosis of made when negative on platelets (type II) or both and monocytes I). Among 827 apparently healthy Japanese volunteers, type I II deficiencies were found in 8 (1.0%) 48 (5.8%), respectively. T for C substitution at nt478 Pro90Ser insertion A nt1159 constituted major causes deficiencies. dinucleotide deletion nt539 had a minor role. In two family studies, we previously unreported...
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class endogenous lipids with interesting physiological functions in mammals. Despite their structural diversity and links nuclear factor erythroid 2-related 2 (NRF2) biosynthesis, FAHFAs less explored as NRF2 activators. Herein, we examined for the first time synthetic docosahexaenoic 12-hydroxy stearic (12-DHAHSA) or oleic (12-DHAHOA) against activation cultured human hepatoma-derived cells (C3A). The effect DHA-derived on lipid...
To find out whether CD36 plays a role in the human lipoprotein metabolism, we studied profiles subjects with deficiency. Apparently healthy Japanese volunteers (n = 790) were classified by flow cytometry into three groups of normal (platelet and monocyte CD36+, n 741, 93.8%), type-II deficiency CD36− 45, 5.7%), type-I CD36−, 4, 0.5%). At least one reported mutations gene was found all four 23 45 type II. Among 779 (731 normals, 44 II, I) serum triglyceride levels <400 mg/dL, total...